1887

Abstract

A highly sensitive immunochromatography test kit, ODK0501, was developed using specific polyclonal antibodies against the C-polysaccharide moiety of for the rapid detection of antigen in sputum samples. The clinical utility of ODK0501 for this detection was evaluated prospectively in 52 adult patients with respiratory infections and compared with that of a urinary antigen detection kit. Overall, 21 patients (40.4 %) showed positive results with ODK0501, compared with 16 patients (30.8 %) using the urinary antigen detection kit, and was cultured from 18 patients. ODK0501 and the urinary antigen detection kit exhibited a sensitivity of 94.4 and 55.6 % (<0.01), respectively, and a specificity of 88.2 and 82.4 %, respectively. Eleven of thirteen patients with conflicting results between the two test kits exhibited consistent results for sputum cultures. Moreover, eight out of nine patients positive for ODK0501 and negative for the urinary antigen detection kit were culture-positive, including five who exhibited phagocytosis, indicating as a causative agent of infection, in Gram staining of sputum samples. These results suggest that the ODK0501 direct sputum detection kit is more clinically useful than the urinary antigen detection kit in adult patients with respiratory infections.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47793-0
2008-07-01
2019-11-21
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/7/820.html?itemId=/content/journal/jmm/10.1099/jmm.0.47793-0&mimeType=html&fmt=ahah

References

  1. Abe, S., Ishihara, K. & Okuda, K. ( 2001; ). Prevalence of potential respiratory pathogens in the mouths of elderly patients and effects of professional oral care. Arch Gerontol Geriatr 32, 45–55.[CrossRef]
    [Google Scholar]
  2. Burman, L. A., Trollfer, B., Andersson, B., Henrichsen, J., Juto, P., Kallings, I., Lagergård, T., Möllby, R. & Norrby, R. ( 1991; ). Diagnosis of pneumonia by cultures, bacterial and viral antigen detection tests, and serology with special reference to antibodies against pneumococcal antigens. J Infect Dis 163, 1087–1093.[CrossRef]
    [Google Scholar]
  3. Dominguez, J., Gali, N., Blanco, S., Pedroso, P., Prat, C., Matas, L. & Ausina, V. ( 2001; ). Detection of Streptococcus pneumoniae antigen by a rapid immunochromatographic assay in urine samples. Chest 119, 243–249.[CrossRef]
    [Google Scholar]
  4. Dowell, S. F., Garman, R. L., Liu, G., Levine, O. S. & Yang, Y. H. ( 2001; ). Evaluation of Binax NOW, an assay for the detection of pneumococcal antigen in urine samples, performed among pediatric patients. Clin Infect Dis 32, 824–825.[CrossRef]
    [Google Scholar]
  5. File, T. M., Jr ( 2003; ). Community-acquired pneumonia. Lancet 362, 1991–2001.[CrossRef]
    [Google Scholar]
  6. Gutiérrez, F., Masia, M., Rodriguez, J. C., Ayelo, A., Soldan, B., Cebrian, L., Mirete, C., Royo, G. & Hidalgo, A. M. ( 2003; ). Evaluation of the immunochromatographic Binax NOW assay for detection of Streptococcus pneumoniae urinary antigen in a prospective study of community-acquired pneumonia in Spain. Clin Infect Dis 36, 286–292.[CrossRef]
    [Google Scholar]
  7. Hamer, D. H., Egas, J., Estrella, B., MacLeod, W. B., Griffiths, J. K. & Semoertegui, F. ( 2002; ). Assessment of the Binax NOW Streptococcus pneumoniae urinary antigen test in children with nasopharyngeal pneumococcal carriage. Clin Infect Dis 34, 1025–1028.[CrossRef]
    [Google Scholar]
  8. Harding, S. A., Scheld, W. M., McGowan, M. D. & Sande, M. A. ( 1979; ). Enzyme-linked immunosorbent assay for detection of Streptococcus pneumoniae antigen. J Clin Microbiol 10, 339–342.
    [Google Scholar]
  9. Henrichsen, J. ( 1995; ). Six newly recognized types of Streptococcus pneumoniae. J Clin Microbiol 33, 2759–2762.
    [Google Scholar]
  10. Ishida, T., Hashimoto, T., Arita, M., Tojo, Y., Tachibana, H. & Jinnai, M. ( 2004; ). A 3-year prospective study of a urinary antigen-detection test for Streptococcus pneumoniae in community-acquired pneumonia: utility and clinical impact on the reported etiology. J Infect Chemother 10, 359–363.[CrossRef]
    [Google Scholar]
  11. Krook, A. & Holmberg, H. ( 1987; ). Pneumococcal antigens in sputa: ELISA for the detection of pneumococcal C-polysaccharide in sputa from pneumonia patients. Diagn Microbiol Infect Dis 7, 73–75.[CrossRef]
    [Google Scholar]
  12. Leeming, J. P., Cartwright, K., Morris, R., Martin, S. A. & Smith, M. D., on behalf of the South-West Pneumococcus Study Group ( 2005; ). Diagnosis of invasive pneumococcal infection by serotype-specific urinary antigen detection. J Clin Microbiol 43, 4972–4976.[CrossRef]
    [Google Scholar]
  13. Lieberman, D., Shleyfer, E., Castle, H., Terry, A., Harman-Boehm, I., Delgado, J., Peled, N. & Lieberman, D. ( 2006; ). Nasopharyngeal versus oropharyngeal sampling for isolation of potential respiratory pathogens in adults. J Clin Microbiol 44, 525–528.[CrossRef]
    [Google Scholar]
  14. Llor, C., Cots, J. M. & Herreras, A. ( 2006; ). Bacterial etiology of chronic bronchitis exacerbations treated by primary care physicians. Arch Bronconeumol 42, 388–393.
    [Google Scholar]
  15. Marcos, M. A., Jimenez de Anta, M. T., de la Bellacasa, J. P., González, J., Martínez, E., García, E., Mensa, J., de Roux, A. & Torres, A. ( 2003; ). Rapid urinary antigen test for diagnosis of pneumococcal community-acquired pneumonia in adults. Eur Respir J 21, 209–214.[CrossRef]
    [Google Scholar]
  16. Miller, D. L. & Jones, R. ( 1963; ). A study of techniques for the examination of sputum in a field survey of chronic bronchitis. Am Rev Respir Dis 88, 473–483.
    [Google Scholar]
  17. Murdoch, D. R. & Reller, L. B. ( 2003; ). Immunochromatographic test for rapid detection of Streptococcus pneumoniae in the nasopharynx. J Clin Microbiol 41, 2271 [CrossRef]
    [Google Scholar]
  18. Murdoch, D. R., Laing, R. T., Mills, G. D., Karalus, N. C., Town, G. I., Mirrett, S. & Reller, L. B. ( 2001; ). Evaluation of a rapid immunochromatographic test for detection of Streptococcus pneumoniae antigen in urine samples from adults with community-acquired pneumonia. J Clin Microbiol 39, 3495–3498.[CrossRef]
    [Google Scholar]
  19. Murdoch, D. R., Laing, R. T. & Cook, J. M. ( 2003; ). The NOW Streptococcus pneumoniae urinary antigen test positivity rate 6 weeks after pneumonia onset and among patients with COPD. Clin Infect Dis 37, 153–154.[CrossRef]
    [Google Scholar]
  20. Murray, P. R. & Washington, J. A. ( 1975; ). Microscopic and bacteriologic analysis of expectorated sputum. Mayo Clin Proc 50, 339
    [Google Scholar]
  21. Navarro, D., Garcia, M. L., Gimeno, C., Escribano, A. & Garcia, L. ( 2004; ). Performance of the Binax NOW Streptococcus pneumoniae urinary antigen assay for diagnosis of pneumonia in children with underlying pulmonary diseases in the absence of acute pneumococcal infection. J Clin Microbiol 42, 4853–4855.[CrossRef]
    [Google Scholar]
  22. Saito, A., Kohno, S., Matsushima, T., Watanabe, A., Oizumi, K., Yamaguchi, K. & Oda, H. the Study Group ( 2006; ). Prospective multicenter study of the causative organisms of community-acquired pneumonia in adults in Japan. J Infect Chemother 12, 63–69.[CrossRef]
    [Google Scholar]
  23. Sorensen, U. B. ( 1995; ). Pneumococcal polysaccharide antigens: capsules and C-polysaccharide. Dan Med Bull 42, 47–53.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47793-0
Loading
/content/journal/jmm/10.1099/jmm.0.47793-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error