1887

Abstract

Previous studies have shown that the LPS of isolated from North American and European hosts predominantly expresses type 2 Lewis x (Le) and Le epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Le and Le structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of isolates were assessed by gel electrophoresis and Western blotting. Expression of Le and/or Le epitopes was confirmed in 32/38 isolates (84 %), whilst 9/38 isolates (24 %) expressed type 1 Le blood group determinants, in addition to type 2 Le and Le structures. Six strains (16 %) were non-typeable. The majority of strains examined were CagA-positive (83.3 %).

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2008-05-01
2019-11-15
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