1887

Abstract

Previous studies have shown that the LPS of isolated from North American and European hosts predominantly expresses type 2 Lewis x (Le) and Le epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Le and Le structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of isolates were assessed by gel electrophoresis and Western blotting. Expression of Le and/or Le epitopes was confirmed in 32/38 isolates (84 %), whilst 9/38 isolates (24 %) expressed type 1 Le blood group determinants, in addition to type 2 Le and Le structures. Six strains (16 %) were non-typeable. The majority of strains examined were CagA-positive (83.3 %).

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47783-0
2008-05-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/5/585.html?itemId=/content/journal/jmm/10.1099/jmm.0.47783-0&mimeType=html&fmt=ahah

References

  1. Castillo-Rojas G., Mazari-Hiriart M., López-Vidal Y. 2004; Helicobacter pylori : focus on CagA and VacA major virulence factors. Salud Publica Mex 46:538–548 [CrossRef]
    [Google Scholar]
  2. Coelho L. G. V., León-Barúa R., Quigley E. M. M. Representatives of the Latin-American National Gastroenterological Societies affiliated with the Inter-American Association of Gastroenterology (AIGE; 2000; Latin-American consensus conference on Helicobacter pylori infection. Am J Gastroenterol 95:2688–2691
    [Google Scholar]
  3. Covacci A., Telford J. L., Del Giudice G., Parsonnet J., Rappuoli R. 1999; Helicobacter pylori virulence and genetic geography. Science 284:1328–1333 [CrossRef]
    [Google Scholar]
  4. Cover T. L., Glupczynski Y., Lage A. P., Burette A., Tummuru M. K. R., Perez-Perez G. I., Blaser M. J. 1995; Serological detection of infection with cagA+ Helicobacter pylori strains. J Clin Microbiol 33:1496–1500
    [Google Scholar]
  5. Dunn B. E., Cohen H., Blaser M. J. 1997; Helicobacter pylori . Clin Microbiol Rev 10:720–741
    [Google Scholar]
  6. Goodman K. J., Correa P., Tenganá-Aux H. J., Ramírez H., DeLany J. P., Pepinosa O. G., Quiñonez M. L., Collazos-Parra T. 1996; Helicobacter pylori infection in the Colombian Andes: a population-based study of transmission pathways. Am J Epidemiol 144:290–299 [CrossRef]
    [Google Scholar]
  7. Holgersson J., Löfling J. 2006; Glycosyltransferases involved in type 1 chain and Lewis antigen biosynthesis exhibit glycan and core chain specificity. Glycobiology 16:584–593 [CrossRef]
    [Google Scholar]
  8. Hopkins R. J., Vial P. A., Ferreccio C., Ovalle J., Prado P., Sotomayor V., Russell R. G., Wasserman S. S., Morris J. G. Jr 1993; Seroprevalence of Helicobacter pylori in Chile: vegetables may serve as one route of transmission. J Infect Dis 168:222–226 [CrossRef]
    [Google Scholar]
  9. Klein P. D., Gilman R. H., Leon-Barua R., Diaz F., O'Brien Smith E., Graham D. Y. 1994; The epidemiology of Helicobacter pylori in Peruvian children between 6 and 30 months of age. Am J Gastroenterol 89:2196–2200
    [Google Scholar]
  10. Logan S. M., Trust T. J. 1984; Structural and antigenic heterogeneity of lipopolysaccharides of Campylobacter jejuni and Campylobacter coli . Infect Immun 45:210–216
    [Google Scholar]
  11. Logan S. M., Conlan J. W., Monteiro M. A., Wakarchuk W. W., Altman E. 2000; Functional genomics of Helicobacter pylori : identification of a β -1,4 galactosyltransferase and generation of mutants with altered lipopolysaccharide. Mol Microbiol 35:1156–1167 [CrossRef]
    [Google Scholar]
  12. Monteiro M. A. 2001; Helicobacter pylori : a wolf in sheep's clothing: the glycotype families of Helicobacter pylori lipopolysaccharides expressing histo-blood groups: structure, biosynthesis, and role in pathogenesis. Adv Carbohydr Chem Biochem 57:99–158
    [Google Scholar]
  13. Monteiro M. A., Chan K. H. N., Rasko D. A., Taylor D. E., Zheng P. Y., Appelmelk B. J., Wirth H.-P., Yang M., Blaser M. J. other authors 1998; Simultaneous expression of type 1 and type 2 Lewis blood group antigens by Helicobacter pylori lipopolysaccharides. J Biol Chem 273:11533–11543 [CrossRef]
    [Google Scholar]
  14. Monteiro M. A., Zheng P., Ho B., Yokota S., Amano K., Pan Z., Berg D. E., Chan K. H., MacLean L. L., Perry M. B. 2000; Expression of histo-blood group antigens by lipopolysaccharides of Helicobacter pylori strains from Asian hosts: the propensity to express type 1 blood-group antigens. Glycobiology 10:701–713 [CrossRef]
    [Google Scholar]
  15. Oliveira A. M. R., Queiroz D. M. M., Rocha G. A., Mendes E. N. 1994; Seroprevalence of Helicobacter pylori infection in children of low socioeconomic level in Belo Horizonte. Brazil. Am J Gastroenterol 89:2201–2204
    [Google Scholar]
  16. Rasko D. A., Wilson T. J. M., Zopf D., Taylor D. E. 2000; Lewis antigen expression and stability in Helicobacter pylori isolated from serial gastric biopsies. J Infect Dis 181:1089–1095 [CrossRef]
    [Google Scholar]
  17. Sheu B.-S., Sheu S.-M., Yang H.-B., Huang A.-H., Wu J.-J. 2003; Host gastric Lewis expression determines the bacterial density of Helicobacter pylori in babA2 genopositive infection. Gut 52:927–932 [CrossRef]
    [Google Scholar]
  18. Simoons-Smit I. M., Appelmelk B. J., Verboom T., Negrini R., Penner J. L., Aspinall G. O., Moran A. P., Fei S. F., Bi-Shan S. other authors 1996; Typing of Helicobacter pylori with monoclonal antibodies against Lewis antigens in lipopolysaccharide. J Clin Microbiol 34:2196–2200
    [Google Scholar]
  19. Tsai C. M., Frasch C. E. 1982; A sensitive silver stain for detecting lipopolysaccharide in polyacrylamide gels. Anal Biochem 119:115–119 [CrossRef]
    [Google Scholar]
  20. Wirth H.-P., Yang M., Karita M., Blaser M. J. 1996; Expression of the human cell surface glycoconjugates Lewis X and Lewis Y by Helicobacter pylori isolates is related to cagA status. Infect Immun 64:4598–4605
    [Google Scholar]
  21. Wirth H.-P., Yang M., Peek R. M. Jr, Tham K. T., Blaser M. J. 1997; Helicobacter pylori Lewis expression is related to the host Lewis phenotype. Gastroenterology 113:1091–1098 [CrossRef]
    [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.47783-0
Loading
/content/journal/jmm/10.1099/jmm.0.47783-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error