1887

Abstract

A mini-Tn transposon insertion in a gene encoding an orthologue of the Lon protease conferred a hyper-swarming phenotype on . The mutation increased the accumulation of mRNA for representative class 1 (), class 2 () and class 3 () genes during swarmer cell differentiation. In addition, the stability of the FlhD protein was fourfold higher in the  : : mini-Tn background. Expression of a single-copy  : :  fusion increased during the swarming cycle and reached peak levels of expression at a point just after swarmer cell differentiation had initiated. In liquid media, a condition normally non-permissive for swarming, the  : : mini-Tn insertion resulted in motile, highly elongated cells that overexpressed flagellin. Finally, the  : : mini-Tn mutation was shown to result in increased expression of the and virulence genes during swarmer cell differentiation.

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2008-08-01
2020-07-08
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