1887

Abstract

This study describes the distribution and frequencies of strain types by protein A-encoding gene () typing among a total of 200 meticillin-resistant (MRSA) single-patient isolates collected between 2000 and 2005 at the University Hospital Basel, Switzerland. Nine frequent types accounted for 49.5 % of MRSA isolates, whereas type t041 (15 % of all isolates) belonged to a local epidemic strain that is also a common strain type in southern Germany. Successful control of the outbreak strain was documented by epidemiological data and confirmed by typing results. The type t044 (3.5 %), corresponding to a widely disseminated European community-acquired MRSA (CA-MRSA), was first observed in 2002. The well-known CA-MRSA USA300 clone was detected in four patients (2 %). Sporadic strains occurring less than four times (32 different types) accounted for 23 % of isolates. No predominant type was seen, indicating a great genetic diversity. Only 34.5 % of patient isolates were acquired nosocomially. The presence of one or more of ten common virulence genes was shown in 79 % of strains. It was demonstrated that the sequence-based typing method allows analysis of local MRSA epidemiology in relation to other regions and countries over time.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47757-0
2008-05-01
2020-09-30
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/5/612.html?itemId=/content/journal/jmm/10.1099/jmm.0.47757-0&mimeType=html&fmt=ahah

References

  1. Aires de Sousa M., Conceicao T., Simas C., de Lencastre H. 2005; Comparison of genetic backgrounds of methicillin-resistant and -susceptible Staphylococcus aureus isolates from Portuguese hospitals and the community. J Clin Microbiol 43:5150–5157 [CrossRef]
    [Google Scholar]
  2. Deurenberg R. H., Vink C., Kalenic S., Friedrich A. W., Bruggeman C. A., Stobberingh E. E. 2007; The molecular evolution of methicillin-resistant Staphylococcus aureus . Clin Microbiol Infect 13:222–235 [CrossRef]
    [Google Scholar]
  3. Garner J. S., Jarvis W. R., Emori T. G., Horan T. C., Hughes J. M. 1988; CDC definitions for nosocomial infections, 1988. Am J Infect Control 16:128–140 [CrossRef]
    [Google Scholar]
  4. Ghebremedhin B., Konig W., Konig B. 2005; Heterogeneity of methicillin-resistant Staphylococcus aureus strains at a German university hospital during a 1-year period. Eur J Clin Microbiol Infect Dis 24:388–398 [CrossRef]
    [Google Scholar]
  5. Hallin M., Deplano A., Denis O., De Mendonca R., De Ryck R., Struelens M. J. 2007; Validation of pulsed-field gel electrophoresis and spa typing for long-term, nationwide epidemiological surveillance studies of Staphylococcus aureus infections. J Clin Microbiol 45:127–133 [CrossRef]
    [Google Scholar]
  6. Harmsen D., Claus H., Witte W., Rothganger J., Claus H., Turnwald D., Vogel U. 2003; Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 41:5442–5448 [CrossRef]
    [Google Scholar]
  7. Lina G., Piemont Y., Godail-Gamot F., Bes M., Peter M. O., Gauduchon V., Vandenesch F., Etienne J. 1999; Involvement of Panton–Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis 29:1128–1132 [CrossRef]
    [Google Scholar]
  8. Mehrotra M., Wang G., Johnson W. M. 2000; Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J Clin Microbiol 38:1032–1035
    [Google Scholar]
  9. Mellmann A., Friedrich A. W., Kipp F., Hinder F., Keckevoet U., Harmsen D. 2005; Evidence-based infection control methods using spa genotyping for MRSA spread in hospitals. Dtsch Med Wochenschr 130:1364–1368 (in German [CrossRef]
    [Google Scholar]
  10. Mertz D., Frei R., Jaussi B., Tietz A., Stebler C., Fluckiger U., Widmer A. F. 2007; Throat swabs are necessary to reliably detect carriers of Staphylococcus aureus . Clin Infect Dis 45:475–477 [CrossRef]
    [Google Scholar]
  11. Murakami K., Minamide W., Wada K., Nakamura E., Teraoka H., Watanabe S. 1991; Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. J Clin Microbiol 29:2240–2244
    [Google Scholar]
  12. Ruppitsch W., Indra A., Stoger A., Mayer B., Stadlbauer S., Wewalka G., Allerberger F. 2006; Classifying spa types in complexes improves interpretation of typing results for methicillin-resistant Staphylococcus aureus . J Clin Microbiol 44:2442–2448 [CrossRef]
    [Google Scholar]
  13. Stranden A., Frei R., Widmer A. F. 2003; Molecular typing of methicillin-resistant Staphylococcus aureus : can PCR replace pulsed-field gel electrophoresis?. J Clin Microbiol 41:3181–3186 [CrossRef]
    [Google Scholar]
  14. Strommenger B., Kettlitz C., Weniger T., Harmsen D., Friedrich A. W., Witte W. 2006; Assignment of Staphylococcus isolates to groups by spa typing, Sma I macrorestriction analysis, and multilocus sequence typing. J Clin Microbiol 44:2533–2540 [CrossRef]
    [Google Scholar]
  15. Tietz A., Frei R., Widmer A. F. 2005; Transatlantic spread of the USA300 clone of MRSA. N Engl J Med 353:532–533 [CrossRef]
    [Google Scholar]
  16. Van Belkum A., Van Leeuwen W., Kaufmann M. E., Cookson B., Forey F., Etienne J., Goering R., Tenover F., Steward C. other authors 1998; Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of Sma I macrorestriction fragments: a multicenter study. J Clin Microbiol 36:1653–1659
    [Google Scholar]
  17. Vandenesch F., Naimi T., Enright M. C., Lina G., Nimmo G. R., Heffernan H., Liassine N., Bes M., Greenland T. other authors 2003; Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton–Valentine leukocidin genes: worldwide emergence. Emerg Infect Dis 9:978–984 [CrossRef]
    [Google Scholar]
  18. Yamasaki O., Tristan A., Yamaguchi T., Sugai M., Lina G., Bes M., Vandenesch F., Etienne J. 2006; Distribution of the exfoliative toxin D gene in clinical Staphylococcus aureus isolates in France. Clin Microbiol Infect 12:585–588 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47757-0
Loading
/content/journal/jmm/10.1099/jmm.0.47757-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error