1887

Abstract

is the main cause of bacterial acute gastroenteritis worldwide. In its colonization of the host intestinal tract, it encounters secreted mucins in the mucus layer and surface mucins in the epithelial cells. Mucins are complex glycoproteins that comprise the major component of mucus and give mucus its viscous consistency. MUC2 is the most abundant secreted mucin in the human intestine; it is a major chemoattractant for , and the bacterium binds to it. There are no studies on the transcriptional response of the bacterium to this mucin. Here, cell-culture techniques and quantitative RT-PCR were used to characterize the effects of MUC2 on growth and the changes in expression of 20 genes related to various functions. The genes encoding cytolethal distending toxin protein (), vacuolating cytotoxin (), lipoprotein (), invasion antigen (), the multidrug efflux system (), putative mucin-degrading enzymes (, , and ), flagellin A () and putative rod-shape-determining proteins ( and ) were upregulated, whereas those encoding adhesion fibronectin-binding protein () and sialic acid synthase () were downregulated. These results showed that utilizes MUC2 as an environmental cue for the modulation of expression of genes with various functions including colonization and pathogenicity.

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2008-07-01
2019-11-12
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