1887

Abstract

The aim of this study was to analyse the frequency and expression of biosynthesis genes in 47 isolates with different mutacin-producing phenotypes. Detection of the frequency and expression of genes encoding mutacin types I, II, III and IV were carried out by PCR and semi-quantitative RT-PCR, respectively, using primers specific for each type of biosynthesis gene. In addition, a further eight genes encoding putative bacteriocins, designated , , , , , , and , were also screened. There was a high phenotypic diversity; some isolates presented broad antimicrobial spectra against other clinical isolates, including bacteria resistant to common antibiotics, as well as , , and . The expression frequency of the gene was higher than that of the previously characterized mutacins (I–IV). There was no positive correlation between the number of indicator strains inhibited (antimicrobial spectra) and the number of biosynthesis genes expressed (Spearman correlation test, =−0.03, >0.05). In conclusion, the high diversity of mutacin-producing phenotypes, associated with high frequency of expression of the biosynthesis genes screened, reveals a broad repertoire of genetic determinants encoding antimicrobial peptides that can act in different combinations.

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2008-05-01
2019-10-16
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