1887

Abstract

In the present study, we examined the roles of natural killer T (NKT) cells in host defence against in a mouse model. The survival rate of NKT cell-deficient J281 knock-out (KO) mice was significantly higher than that of wild-type mice. There was no bacterial overgrowth in the lungs, but J281 KO mice showed enhanced pulmonary clearance at a later stage of infection, compared with their wild-type counterparts. The severity of lung injury in -infected J281 KO mice was less, as indicated by lung permeability measurements, such as lung weight and bronchoalveolar lavage fluid albumin concentration. Recruitment of inflammatory cells in the lungs was approximately twofold greater in J281 KO mice on day 3. Interestingly, higher values of interleukin (IL)-1 and IL-18, and increased caspase-1 activity were noted in the lungs of J281 KO mice from an early time point (6 h). Exogenous -galactosylceramide, a ligand of NKT cells, induced IL-12 and gamma interferon at 6 h, but suppressed IL-1 at later time points in wild-type, whereas no effects were evident in J281 KO mice, as expected. Systemic administration of heat-killed , but not LPS, reproduced exaggerated production of IL-1 in the lungs of J281 KO mice. These results demonstrate that NKT cells play a role in host defence against , which is characterized by enhanced lung injury and decreased accumulation of inflammatory cells in the lungs. The regulation of IL-1, IL-18 and caspase-1 may be associated with the modulating effect of host responses by NKT cells.

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2008-11-01
2019-10-22
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