1887

Abstract

Forty-eight isolates collected from a young, healthy, Irish university student population from 1995 to 2004 were screened for 16 enterotoxin (SE) and enterotoxin-like (SEl) genes (, , , , ), and for the toxic shock toxin syndrome toxin-1 gene, . All of the isolates harboured at least one SE or SEl gene and 66.7 % possessed a classical SE gene (, , ), the commonest being the gene. Most of the isolates (85.4 %) had a complete locus (, , , , ). The intergenic region of the locus was typed by PCR-RFLP in 34 isolates, 15 possessing pseudogenes and and 19 having the gene. The and genes, the gene combination, and the gene were each found in <15 % of isolates. The genotype distribution was type III, 37.5 %; type I, 35.4 %; type II, 25 %; and type IV, 2.1 %. There was no association between SE–SEl genotype and type. All gene-positive isolates were of type III and harboured a classical SE gene. Multiple locus, variable number tandem repeat analysis (MLVA) produced 47 different patterns. While the locus was present in all isolates, half of them lacked one or two of the gene amplimers. Twenty isolates harboured the gene, its presence being associated with type III, but not with the SE–SEl gene profile. The types of isolates were associated with MLVA subclusters. Selective MLST analysis revealed seven novel sequence types and a new allele. Five clonal clusters (CCs), including CCs comprising major pandemic clones CC30, CC5 and CC22 and minor lineages CC6 and CC9, and three singletons were identified.

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2008-03-01
2019-10-21
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