1887

Abstract

Human granulocytic anaplasmosis (HGA) and Lyme borreliosis (LB) are tick-borne infectious diseases caused by and species, respectively. In this study, / paralogues specific to and 5S–23S rRNA gene-intergenic spacers specific to species were detected by PCR in ticks collected in two regions, Tver (Kalinin) and Konakovo, of the Tver (Kalinin) Province located 150 km north-west of Moscow. The PCR amplicons obtained were further characterized by sequencing and RFLP analysis. In the total of 199 ticks collected, 8.8 % (7/80) and 33.8 % (27/80) of , and 2.5 % (3/119) and 45.4 % (54/119) of , were found to be infected with and spp., respectively. Of those 199 ticks, 5 (2.5 %) were coinfected with and . Phylogenetic analysis revealed unique / paralogous genes in -infected Russian ticks. The sequence similarities with those of in the United States, UK and Japan ranged from 42 % to 80.4 %, and there were no sequences showing more than 90 % similarity with those sequences from the other countries. The results showed that the / sequence similarity groups may provide an index of adaptation of strains to specific vector ticks or reservoir hosts in different countries and areas. These findings suggest that there is a public health threat from HGA and LB in Tver Province surrounding Moscow.

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2008-08-01
2021-02-25
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