This study investigated whether an improvement in periodontal health resulted in changes in the prevalence of periodontopathogenic bacteria in saliva and tongue coatings and a reduction in volatile sulfur compounds (VSCs: H2S and CH3SH) linked to oral malodour. The subjects were 35 patients who visited the breath odour clinic of Kyushu Dental College, Japan. Their mean age was 51.2±18.3 years (mean±sd). A clinical examination performed at baseline and 2 months after periodontal treatment assessed VSCs in mouth air using gas chromatography, periodontal probing depth and bleeding on probing (BOP) in all subjects; saliva and tongue coatings were also collected. Genomic DNA was isolated from the samples, and the proportions of five periodontopathogenic bacteria (Porphyromonas gingivalis, Tannerella forsythensis, Treponema denticola, Prevotella intermedia and Prevotella nigrescens) were investigated using quantitative real-time PCR. The subjects were classified into four groups based on the presence of a periodontal pocket of more than 4 mm (PD) and VSCs above the organoleptic threshold level (VSCT) as follows: –PD/–VSCT group, subjects without PD or VSCT; –PD/+VSCT group, those without PD but with VSCT; +PD/–VSCT group, those with PD but without VSCT; and +PD/+VSCT group, those with PD and VSCT. Although the mean PD values in the +PD/–VSCT and +PD/+VSCT groups, BOP in the +PD/+VSCT group, and H2S and CH3SH concentrations in the –PD/+VSCT and +PD/+VSCT groups were greater than in the other groups at baseline, we found no significant difference among the four groups after periodontal treatment. The proportion of periodontopathogenic bacteria in saliva was higher in the +PD/–VSCT and +PD/+VSCT groups than in the –PD/–VSCT and –PD/+VSCT groups at baseline and after treatment, but the proportions of bacteria in saliva after treatment were reduced compared to the baseline. Furthermore, the differences in the proportions of the five target bacteria in the tongue coating were not as apparent as those in saliva at baseline or after treatment. The prevalence of periodontopathogenic bacteria in saliva may reflect periodontal health status and influence VSC levels in mouth air.
AwanoS.,
GohanaK.,
KuriharaE.,
AnsaiT.,
TakeharaT.2002; The relationship between the presence of periodontopathogenic bacteria in saliva and halitosis. Int Dent J 52:212–216[CrossRef]
KazorC. E.,
MitchellP. M.,
LeeA. M.,
StokesL. N.,
LoescheW. J.,
DewhirstF. E.,
PasterB. J.2003; Diversity of bacterial populations on the tongue dorsa of patients with halitosis and healthy patients. J Clin Microbiol 41:558–563[CrossRef]
KozlovskyA.,
GordonD.,
GelernterI.,
LoescheW. J.,
RosenbergM.1994; Correlation between the BANA test and oral malodor parameters. J Dent Res 73:1036–1042
Mantilla GomezS.,
DanserM. M.,
SiposP. M.,
RowshaniB.,
van der VeldenU.,
van der WeijdenG. A.2001; Tongue coating and salivary bacterial counts in healthy/gingivitis subjects and periodontitis patients. J Clin Periodontol 28:970–978[CrossRef]
MiyazakiH.,
SakaoS.,
KatohY.,
TakeharaT.1995; Correlation between volatile sulphur compounds and certain oral health measurements in the general population. J Periodontol 66:679–684[CrossRef]
PasterB. J.,
BochesS. K.,
GalvinJ. L.,
EricsonR. E.,
LauC. N.,
LevanosV. A.,
SahasrabudheA.,
DewhirstF. E.2001; Bacterial diversity in human subgingival plaque. J Bacteriol 183:3770–3783[CrossRef]
PerssonS.,
EdlundM. B.,
ClaessonR.,
CarlssonJ.1990; The formation of hydrogen sulfide and methyl mercaptan by oral bacteria. Oral Microbiol Immunol 5:195–201[CrossRef]
SuzukiN.,
YoshidaA.,
SaitoT.,
KawadaM.,
NakanoY.2004; Quantitative microbiological study of subgingival plaque by real-time PCR shows correlation between levels of Tannerella forsythensis and Fusobacterium spp. J Clin Microbiol 42:2255–2257[CrossRef]
TannerA. C.,
PasterB. J.,
LuS. C.,
KanasiE.,
KentR.Jr,
Van DykeT.,
SonisS. T.2006; Subgingival and tongue microbiota during early periodontitis. J Dent Res 85:318–323[CrossRef]
UmedaM.,
ContrerasA.,
ChenC.,
BakkerI.,
SlotsJ.1998; The utility of whole saliva to detect the oral presence of periodontopathic bacteria. J Periodontol 69:828–833[CrossRef]
WashioJ.,
SatoT.,
KosekiT.,
TakahashiN.2005; Hydrogen sulfide-producing bacteria in tongue biofilm and their relationship with oral malodour. J Med Microbiol 54:889–895[CrossRef]
YaegakiK.,
SanadaK.1992b; Volatile sulfur compounds in mouth air from clinically healthy subjects and patients with periodontal disease. J Periodontal Res 27:233–238[CrossRef]
YangH. W.,
HuangY. F.,
ChouM. Y.2004; Occurrence of Porphyromonas gingivalis and Tannerella forsythensis in periodontally diseased and healthy subjects. J Periodontol 75:1077–1083[CrossRef]
YoshidaY.,
SuzukiN.,
NakanoY.,
ShibuyaK.,
OgawaY.,
KogaT.2003; Distribution of Actinobacillus actinomycetemcomitans serotypes and Porphyromonas gingivalis in Japanese adults. Oral Microbiol Immunol 18:135–139[CrossRef]
YoshidaA.,
KawadaM.,
SuzukiN.,
NakanoY.,
OhoT.,
SaitoT.,
YamashitaY.2004; TaqMan real-time polymerase chain reaction assay for the correlation of Treponema denticola numbers with the severity of periodontal disease. Oral Microbiol Immunol 19:196–200[CrossRef]