1887

Abstract

A total of 313 faecal samples from three pig farms and two cattle farms was collected, and was isolated from 133/257 piglet samples (51.8 %) and from 1/56 calf samples (1.8 %). Toxins were tested only in calf samples and were positive in 44.6 % (25/56). The only bovine isolate belonged to toxinotype XIa (ABCDT). Porcine isolates belonged to toxinotype 0 (ABCDT) and toxinotype V (ABCDT). Of the two ribotypes usually found in toxinotype V, the strains isolated in this study showed a greater similarity to ribotype 066 than to ribotype 078.

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2008-06-01
2021-02-28
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References

  1. Arroyo L. G., Kruth S. A., Willey B. M., Staempfli H. R., Low D. E., Weese J. S. 2005; PCR ribotyping of Clostridium difficile isolates originating from human and animal sources. J Med Microbiol 54:163–166 [CrossRef]
    [Google Scholar]
  2. Bartlett J. G. 1992; Antibiotic-associated diarrhea. Clin Infect Dis 15:573–581 [CrossRef]
    [Google Scholar]
  3. Baverud V. 2002; Clostridium difficile infections in animals with special reference to the horse. A review. Vet Q 24:203–219 [CrossRef]
    [Google Scholar]
  4. Bidet P., Barbut F., Lalande V., Burghoffer B., Petit J. C. 1999; Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing. FEMS Microbiol Lett 175:261–266 [CrossRef]
    [Google Scholar]
  5. Borriello S. P., Honour P., Turner T., Barclay F. 1983; Household pets as a potential reservoir for Clostridium difficile infection. J Clin Pathol 36:84–87 [CrossRef]
    [Google Scholar]
  6. Braun V., Hundesberger T., Leukel P., Sauerborn M., von Eichel–Streiber C. 1996; Definition of the single integration site of the pathogenicity locus in Clostridium difficile . Gene 181:29–38 [CrossRef]
    [Google Scholar]
  7. Keel K., Brazier J. S., Post K. W., Weese S., Songer J. G. 2007; Prevalence of PCR ribotypes among Clostridium difficile isolates from pigs, calves, and other species. J Clin Microbiol 45:1963–1964 [CrossRef]
    [Google Scholar]
  8. Kiss D., Bilkei G. 2005; A new periparturient disease in Eastern Europe, Clostridium difficile causes postparturient sow losses. Theriogenology 63:17–23 [CrossRef]
    [Google Scholar]
  9. Lefebvre S. L., Arroyo L. G., Weese J. S. 2006a; Epidemic Clostridium difficile strain in hospital visitation dog. Emerg Infect Dis 12:1036–1037 [CrossRef]
    [Google Scholar]
  10. Lefebvre S. L., Waltner-Toews D., Peregrine A. S., Reid-Smith R., Hodge L., Arroyo L. G., Weese J. S. 2006b; Prevalence of zoonotic agents in dogs visiting hospitalized people in Ontario: implications for infection control. J Hosp Infect 62:458–466 [CrossRef]
    [Google Scholar]
  11. Marks S. L., Kather E. J. 2003; Antimicrobial susceptibilities of canine Clostridium difficile and Clostridium perfringens isolates to commonly utilized antimicrobial drugs. Vet Microbiol 94:39–45 [CrossRef]
    [Google Scholar]
  12. McDonald L. C., Owings M., Jernigan D. B. 2006; Clostridium difficile infection in patients discharged from US short-stay hospitals, 1996–2003. Emerg Infect Dis 12:409–415 [CrossRef]
    [Google Scholar]
  13. O'Neill G., Adams J. E., Bowman R. A., Riley T. V. 1993; A molecular characterization of Clostridium difficile isolates from humans, animals and their environments. Epidemiol Infect 111:257–264 [CrossRef]
    [Google Scholar]
  14. Rodriguez-Palacios A., Staempfli H. R., Duffield T., Peregrine A. S., Trotz-Williams L. A., Arroyo L. G., Brazier J. S., Weese J. S. 2006; Clostridium difficile PCR ribotypes in calves, Canada. Emerg Infect Dis 12:1730–1736 [CrossRef]
    [Google Scholar]
  15. Rodriguez-Palacios A., Staempfli H. R., Duffield T., Weese J. S. 2007; Clostridium difficile in retail ground meat, Canada. Emerg Infect Dis 13:485–487 [CrossRef]
    [Google Scholar]
  16. Rupnik M. 2007; Is Clostridium difficile -associated infection a potentially zoonotic and foodborne disease?. Clin Microbiol Infect 13:457–459 [CrossRef]
    [Google Scholar]
  17. Rupnik M., Avesani V., Janc M., von Eichel–Streiber C., Delmée M. 1998; A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J Clin Microbiol 36:2240–2247
    [Google Scholar]
  18. Rupnik M., Brazier J., Duerden B., Grabnar M., Stubbs S. 2001; Comparison of toxinotyping and PCR ribotyping of Clostridium difficile strains and description of novel toxinotypes. Microbiology 147:439–447
    [Google Scholar]
  19. Songer J. G., Anderson M. A. 2006; Clostridium difficile : an important pathogen of food animals. Anaerobe 12:1–4 [CrossRef]
    [Google Scholar]
  20. Songer J. G., Post K. W., Larson D. J., Jost B. H., Glock R. D. 2000; Infection of neonatal swine with Clostridium difficile . Swine Health Prod 8:185–189
    [Google Scholar]
  21. Stubbs S. L., Brazier J. S., O'Neill G. L., Duerden B. I. 1999; PCR targeted to the 16S–23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes. J Clin Microbiol 37:461–463
    [Google Scholar]
  22. Stubbs S., Rupnik M., Gibert M., Brazier J., Duerden B., Popoff M. 2000; Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of Clostridium difficile . FEMS Microbiol Lett 186:307–312 [CrossRef]
    [Google Scholar]
  23. Weese J. S., Staempfli H. R., Prescott J. F. 2001a; A prospective study of the roles of Clostridium difficile and enterotoxigenic Clostridium perfringens in equine diarrhoea. Equine Vet J 33:403–409
    [Google Scholar]
  24. Weese J. S., Staempfli H. R., Prescott J. F., Kruth S. A., Greenwood S. J., Weese H. E. 2001b; The roles of Clostridium difficile and enterotoxigenic Clostridium perfringens in diarrhea in dogs. J Vet Intern Med 15:374–378 [CrossRef]
    [Google Scholar]
  25. Weese J. S., Rousseau J., Arroyo L. 2005; Bacteriological evaluation of commercial canine and feline raw diets. Can Vet J 46:513–516
    [Google Scholar]
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