1887

Abstract

In this study, two free-living amoebae strains, genotype T4 and sp., which were isolated from keratitis cases are presented. While the strain was isolated as a single agent, the strain was found together with herpes simplex virus. Neither of the patients were contact lens wearers, but they did have a history of minor corneal trauma. Amoebae were detected on non-nutrient agar covered with . Based on PCR-amplified 18S rRNA-gene analysis the first isolate was identified as genotype T4 and the second as sp. In thermotolerance tests, the maximum temperature at which trophozoites continued to divide was determined as 37 °C for this strain and 35 °C for the strain. To the best of our knowledge, the A strain described herein is the second molecularly identified strain in an keratitis patient in Turkey. However, the isolate is believed to be the first strain that has been isolated from a keratitis patient and has been molecularly differentiated from .

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2008-03-01
2019-11-13
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