1887

Abstract

, the aetiological agent of anthrax, has been taxonomically classified with the group, which comprises , , , and . Although the pathogenesis and ecological manifestations may be different, shares a high degree of DNA sequence similarity with its group member species. As a result, the discrimination of from its close relatives in the group is still quite difficult. Suppression subtractive hybridization (SSH) was performed to search for genomic differences between a Korean isolate CR and the most closely related type strain KCTC 3624. Two-hundred and five CR clones obtained by SSH underwent Southern hybridization, and comparative sequences were analysed using the program from the National Center for Biotechnology Information (NCBI). Subsequently, primer sets based on the glycosyltransferase group 1 family protein gene specific to were designed from the sequences of subtracted clones, and their specificities were evaluated using eight , 33 , 10 , six , one , one and 19 strains from 11 other representative species. PCR primers specific for the glycosyltransferase group 1 family protein gene did not amplify the desired products from any of the strains under examination, except alone. These findings may be useful in the future development of efficient diagnostic tools for the rapid identification of from other members of the group.

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2008-03-01
2019-10-16
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