1887

Abstract

The sequence diversity of genes among group isolates was analysed, including five reference strains and 36 clinical isolates. Phylogenetic analysis of the and sequences showed that the isolates that belonged to the same species or subspecies usually clustered together. The intergenic spacer region between and was variable in size (67–342 bp) and sequence and appeared to be a unique marker for species or subspecies determination. Sequence similarities of the genes among species and subspecies ranged from 84.2 to 99.0 % in , and from 88.0 to 99.0 % in . Based on the sequences determined, a group-specific PCR assay was developed, which may provide an alternative means of distinguishing the bovis group from other viridans streptococci. Restriction digestion of the amplicon with I further differentiated the species and subspecies.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47628-0
2008-04-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/4/432.html?itemId=/content/journal/jmm/10.1099/jmm.0.47628-0&mimeType=html&fmt=ahah

References

  1. Bochud, P. Y., Calandra, T. & Francioli, P. ( 1994; ). Bacteremia due to viridans streptococci in neutropenic patients: a review. Am J Med 97, 256–264.[CrossRef]
    [Google Scholar]
  2. Chadfield, M. S., Christensen, J. P., Decostere, A., Christensen, H. & Bisgaard, M. ( 2007; ). Geno- and phenotypic diversity of avian isolates of Streptococcus gallolyticus subsp. gallolyticus (Streptococcus bovis) and associated diagnostic problems. J Clin Microbiol 45, 822–827.[CrossRef]
    [Google Scholar]
  3. Clarridge, J. E., III, Attorri, S. M., Zhang, Q. & Bartell, J. ( 2001; ). 16S ribosomal DNA sequence analysis distinguishes biotypes of Streptococcus bovis: Streptococcus bovis Biotype II/2 is a separate genospecies and the predominant clinical isolate in adult males. J Clin Microbiol 39, 1549–1552.[CrossRef]
    [Google Scholar]
  4. Coykendall, A. L. ( 1989; ). Classification and identification of the viridans streptococci. Clin Microbiol Rev 2, 315–328.
    [Google Scholar]
  5. Ellmerich, S., Scholler, M., Duranton, B., Gosse, F., Galluser, M., Klein, J. P. & Raul, F. ( 2000; ). Promotion of intestinal carcinogenesis by Streptococcus bovis. Carcinogenesis 21, 753–756.[CrossRef]
    [Google Scholar]
  6. Facklam, R. ( 2002; ). What happened to the streptococci: overview of taxonomic and nomenclature changes. Clin Microbiol Rev 15, 613–630.[CrossRef]
    [Google Scholar]
  7. Farrow, J. A. E., Kruze, J., Phillips, B. A., Bramley, A. J. & Collins, M. D. ( 1984; ). Taxonomic studies on Streptococcus bovis and Streptococcus equinus: description of Streptococcus alactolyticus sp. nov. and Streptococcus saccharolyticus sp. nov. Syst Appl Microbiol 5, 467–482.[CrossRef]
    [Google Scholar]
  8. Gavin, P. J., Warren, J. R., Obias, A. A., Collins, S. M. & Peterson, L. R. ( 2002; ). Evaluation of the Vitek 2 system for rapid identification of clinical isolates of gram-negative bacilli and members of the family Streptococcaceae. Eur J Clin Microbiol Infect Dis 21, 869–874.
    [Google Scholar]
  9. Gerber, J. S., Glas, M., Frank, G. & Shah, S. S. ( 2006; ). Streptococcus bovis infection in young infants. Pediatr Infect Dis J 25, 1069–1073.[CrossRef]
    [Google Scholar]
  10. Goh, S. H., Potter, S., Wood, J. O., Hemmingsen, S. M., Reynolds, R. P. & Chow, A. W. ( 1996; ). HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci. J Clin Microbiol 34, 818–823.
    [Google Scholar]
  11. Goh, S. H., Driedger, D., Gillett, S., Low, D. E., Hemmingsen, S. M., Amos, M., Chan, D., Lovgren, M., Willey, B. M. & other authors ( 1998; ). Streptococcus iniae, a human and animal pathogen: specific identification by the chaperonin 60 gene identification method. J Clin Microbiol 36, 2164–2166.
    [Google Scholar]
  12. Hemmingsen, S. M., Woolford, C., van der Vies, S. M., Tilly, K., Dennis, D. T., Georgopoulos, C. P., Hendrix, R. W. & Ellis, R. J. ( 1988; ). Homologous plant and bacterial proteins chaperone oligomeric protein assembly. Nature 333, 330–334.[CrossRef]
    [Google Scholar]
  13. Hung, W.-C., Tsai, J.-C., Hsueh, P.-R., Chia, J.-S. & Teng, L.-J. ( 2005; ). Species identification of mutans streptococci by groESL gene sequence. J Med Microbiol 54, 857–862.[CrossRef]
    [Google Scholar]
  14. Jean, S. S., Teng, L. J., Hsueh, P. R., Ho, S. W. & Luh, K. T. ( 2004; ). Bacteremic Streptococcus bovis infections at a university hospital, 1992–2001. J Formos Med Assoc 103, 118–123.
    [Google Scholar]
  15. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  16. Kupferwasser, I., Dariu, H., Muller, A. M., Mohr-Kahaly, S., Westermeier, T., Oelert, H., Erbel, R. & Meyer, J. ( 1998; ). Clinical and morphological characteristics in Streptococcus bovis endocarditis: a comparison with other causative microorganisms in 177 cases. Heart 80, 276–280.[CrossRef]
    [Google Scholar]
  17. Lee, J. H., Park, H. S., Jang, W. J., Koh, S. E., Kim, J. M., Shim, S. K., Park, M. Y., Kim, Y. W., Kim, B. J. & other authors ( 2003; ). Differentiation of rickettsiae by groEL gene analysis. J Clin Microbiol 41, 2952–2960.[CrossRef]
    [Google Scholar]
  18. Marston, E. L., Sumner, J. W. & Regnery, R. L. ( 1999; ). Evaluation of intraspecies genetic variation within the 60 kDa heat-shock protein gene (groEL) of Bartonella species. Int J Syst Bacteriol 49, 1015–1023.[CrossRef]
    [Google Scholar]
  19. Osawa, R., Fujisawa, T. & Sly, L. L. ( 1995; ). Streptococcus gallolyticus sp. nov.; gallate degrading organisms formerly assigned to Streptococcus bovis. Syst Appl Microbiol 18, 74–78.[CrossRef]
    [Google Scholar]
  20. Poyart, C., Quesne, G., Coulon, S., Berche, P. & Trieu-Cuot, P. ( 1998; ). Identification of streptococci to species level by sequencing the gene encoding the manganese-dependent superoxide dismutase. J Clin Microbiol 36, 41–47.
    [Google Scholar]
  21. Poyart, C., Quesne, G. & Trieu-Cuot, P. ( 2002; ). Taxonomic dissection of the Streptococcus bovis group by analysis of manganese-dependent superoxide dismutase gene (sodA) sequences: reclassification of ‘Streptococcus infantarius subsp. coli’ as Streptococcus lutetiensis sp. nov. and of Streptococcus bovis biotype 11.2 as Streptococcus pasteurianus sp. nov. Int J Syst Evol Microbiol 52, 1247–1255.[CrossRef]
    [Google Scholar]
  22. Rastogi, N., Goh, K. S. & Berchel, M. ( 1999; ). Species-specific identification of Mycobacterium leprae by PCR-restriction fragment length polymorphism analysis of the hsp65 gene. J Clin Microbiol 37, 2016–2019.
    [Google Scholar]
  23. Ruoff, K. L., Miller, S. I., Garner, C. V., Ferraro, M. J. & Calderwood, S. B. ( 1989; ). Bacteremia with Streptococcus bovis and Streptococcus salivarius: clinical correlates of more accurate identification of isolates. J Clin Microbiol 27, 305–308.
    [Google Scholar]
  24. Sasaki, E., Osawa, R., Nishitani, Y. & Whiley, R. A. ( 2004; ). Development of a diagnostic PCR assay targeting the Mn-dependent superoxide dismutase gene (sodA) for identification of Streptococcus gallolyticus. J Clin Microbiol 42, 1360–1362.[CrossRef]
    [Google Scholar]
  25. Schlegel, L., Grimont, F., Collins, M. D., Regnault, B., Grimont, P. A. & Bouvet, A. ( 2000; ). Streptococcus infantarius sp. nov., Streptococcus infantarius subsp. infantarius subsp. nov. and Streptococcus infantarius subsp. coli subsp. nov., isolated from humans and food. Int J Syst Evol Microbiol 50, 1425–1434.[CrossRef]
    [Google Scholar]
  26. Schlegel, L., Grimont, F., Ageron, E., Grimont, P. A. & Bouvet, A. ( 2003; ). Reappraisal of the taxonomy of the Streptococcus bovis/Streptococcus equinus complex and related species: description of Streptococcus gallolyticus subsp. gallolyticus subsp. nov., S. gallolyticus subsp. macedonicus subsp. nov. and S. gallolyticus subsp. pasteurianus subsp. nov. Int J Syst Evol Microbiol 53, 631–645.[CrossRef]
    [Google Scholar]
  27. Sumner, J. W., Storch, G. A., Buller, R. S., Liddell, A. M., Stockham, S. L., Rikihisa, Y., Messenger, S. & Paddock, C. D. ( 2000; ). PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris. J Clin Microbiol 38, 2746–2749.
    [Google Scholar]
  28. Teng, L. J., Hsueh, P. R., Ho, S. W. & Luh, K. T. ( 2001a; ). High prevalence of inducible erythromycin resistance among Streptococcus bovis isolates in Taiwan. Antimicrob Agents Chemother 45, 3362–3365.[CrossRef]
    [Google Scholar]
  29. Teng, L. J., Hsueh, P. R., Wang, Y. H., Lin, H. M., Luh, K. T. & Ho, S. W. ( 2001b; ). Determination of Enterococcus faecalis groESL full-length sequence and application for species identification. J Clin Microbiol 39, 3326–3331.[CrossRef]
    [Google Scholar]
  30. Teng, L. J., Hsueh, P. R., Tsai, J. C., Chen, P. W., Hsu, J. C., Lai, H. C., Lee, C. N. & Ho, S. W. ( 2002; ). groESL sequence determination, phylogenetic analysis, and species differentiation for viridans group streptococci. J Clin Microbiol 40, 3172–3178.[CrossRef]
    [Google Scholar]
  31. Tripodi, M. F., Adinolfi, L. E., Ragone, E., Durante Mangoni, E., Fortunato, R., Iarussi, D., Ruggiero, G. & Utili, R. ( 2004; ). Streptococcus bovis endocarditis and its association with chronic liver disease: an underestimated risk factor. Clin Infect Dis 38, 1394–1400.[CrossRef]
    [Google Scholar]
  32. Tsai, J. C., Hsueh, P. R., Lin, H. M., Chang, H. J., Ho, S. W. & Teng, L. J. ( 2005; ). Identification of clinically relevant Enterococcus species by direct sequencing of groES and spacer region. J Clin Microbiol 43, 235–241.[CrossRef]
    [Google Scholar]
  33. Tung, S. K., Teng, L. J., Vaneechoutte, M., Chen, H. M. & Chang, T. C. ( 2007; ). Identification of species of Abiotrophia, Enterococcus, Granulicatella and Streptococcus by sequence analysis of the ribosomal 16S–23S intergenic spacer region. J Med Microbiol 56, 504–513.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47628-0
Loading
/content/journal/jmm/10.1099/jmm.0.47628-0
Loading

Data & Media loading...

Supplements

GenBank/EMBL/DDBJ accession numbers for the and gene sequences of strains used in this study. [PDF](19 KB)

PDF

Nucleotide lengths and sequences of - spacer regions. [PDF](41 KB)

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error