1887

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Volume 57, Issue 4

PCR-RFLP assay for species and subspecies differentiation of the group based on sequences Free

Abstract

The sequence diversity of genes among group isolates was analysed, including five reference strains and 36 clinical isolates. Phylogenetic analysis of the and sequences showed that the isolates that belonged to the same species or subspecies usually clustered together. The intergenic spacer region between and was variable in size (67–342 bp) and sequence and appeared to be a unique marker for species or subspecies determination. Sequence similarities of the genes among species and subspecies ranged from 84.2 to 99.0 % in , and from 88.0 to 99.0 % in . Based on the sequences determined, a group-specific PCR assay was developed, which may provide an alternative means of distinguishing the bovis group from other viridans streptococci. Restriction digestion of the amplicon with I further differentiated the species and subspecies.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ITS, intergenic spacer
SGM
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2008-04-01
2024-04-19
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PCR-RFLP assay for species and subspecies differentiation of the Streptococcus bovis group based on groESL sequences
J. Med. Microbiol. 57, 432 (2008); https://doi.org/10.1099/jmm.0.47628-0
/content/journal/jmm/10.1099/jmm.0.47628-0
/content/journal/jmm/10.1099/jmm.0.47628-0
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