1887

Abstract

An imipenem-resistant isolate of ZJ163 (MIC 256 μg ml) isolated from a Chinese hospital was investigated. The ZJ163 isolate exhibited high-level resistance to carbapenems, penicillins, cephalosporins, cefoxitin, aztreonam, quinolones and aminoglycosides. Isoelectric focusing (IEF) demonstrated three -lactamases with pIs of 5.4 (TEM-1), 6.7 (KPC-2) and 7.9 (CTX-M-14). Two different transconjugants (types A and B) were obtained by conjugation studies. The type A transconjugant exhibited reduced susceptibility or resistance to penicillins, cephalosporins and aztreonam, but was susceptible to carbapenems, quinolones and aminoglycosides. The antimicrobial susceptibility patterns of the type B transconjugant were similar to that of type A, except for its significantly reduced carbapenem susceptibility (imipenem MIC 2 μg ml). IEF, specific PCRs and DNA sequence analysis indicated that the type A transconjugant produced CTX-M-14 -lactamase with a pI of 7.9, that the type B transconjugant produced KPC-2 -lactamase with a pI of 6.7 and that the -lactamase with a pI of 5.4 was TEM-1. PCR analysis and sequencing confirmed the presence of the gene in the chromosomal DNA from ZJ163, although no activity of AmpC -lactamase was detected by IEF. Urea/SDS-PAGE analysis of outer-membrane proteins revealed that the levels of the 41 and 38 kDa porins were decreased in ZJ163. It was concluded that production of KPC-2 combined with decreased expression of porins contributes to high-level resistance to carbapenems in ZJ163.

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2008-03-01
2020-04-06
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