1887

Abstract

A key step in K1 meningitis is the crossing of the blood-brain barrier by the bacteria in order to gain entry into the central nervous system (CNS). In this study, a novel model to study K1 invasion of the CNS is described that uses the African migratory locust, . By injecting bacteria into isolated locust head capsules, it was demonstrated that K1 invade the locust brain within 2 h in numbers depending on the concentration of bacteria injected. Using several mutants derived from K1, it was shown that outer-membrane protein A is a critical bacterial determinant required for the K1 invasion. The isogenic gene-deletion mutants, Δ, Δ, Δ and a rough LPS mutant showed significantly reduced invasion of locust brain. This novel model for the study of K1 pathogenesis offers several advantages over existing mammalian models in relation to its relative ease of use, cost-effectiveness and ethical acceptability.

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2008-01-01
2019-11-21
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