1887

Abstract

species are emerging opportunistic pathogens capable of causing chronic lung infections in cystic fibrosis patients. This study examined the interactions of 17 isolates from the five identified species ( , , , and ) plus two genomospecies isolates with lung epithelial cells and their ability to form biofilms . Only three isolates showed an ability to invade A549 lung epithelial cells, and only one isolate was able to form biofilms. In contrast, all isolates triggered a pronounced pro-inflammatory response, with elevation of both interleukin (IL)-6 (two- to 19-fold) and IL-8 (10- to 50-fold) above that observed for a control strain of . This property is likely to be a major factor in the pathogenesis of the genus.

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2008-01-01
2024-04-19
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References

  1. Atkinson R. M., Lipuma J. J., Rosenbluth D. B., Dunne W. M. Jr 2006; Chronic colonization with Pandoraea apista in cystic fibrosis patients determined by repetitive-element-sequence PCR. J Clin Microbiol 44:833–836 [CrossRef]
    [Google Scholar]
  2. Bonfield T. L., Panuska J. R., Konstan M. W., Hilliard K. A., Hilliard J. B., Ghnaim H., Berger M. 1995; Inflammatory cytokines in cystic fibrosis lungs. Am J Respir Crit Care Med 152:2111–2118 [CrossRef]
    [Google Scholar]
  3. Burns J. L., Jonas M., Chi E. Y., Clark D. K., Berger A., Griffith A. 1996; Invasion of respiratory epithelial cells by Burkholderia ( Pseudomonas ) cepacia . Infect Immun 64:4054–4059
    [Google Scholar]
  4. Caraher E., Duff C., Mullen T., Mc Keon S., Murphy P., Callaghan M., McClean S. 2007a; Invasion and biofilm formation of Burkholderia dolosa is comparable with Burkholderia cenocepacia and Burkholderia multivorans . J Cyst Fibros 6:49–56 [CrossRef]
    [Google Scholar]
  5. Caraher E., Reynolds G., Murphy P., McClean S., Callaghan M. 2007b; Comparison of antibiotic susceptibility of Burkholderia cepacia complex organisms when grown planktonically or as biofilm in vitro. Eur J Clin Microbiol Infect Dis 26:213–216 [CrossRef]
    [Google Scholar]
  6. Chernish R. N., Aaron S. D. 2003; Approach to resistant Gram-negative bacterial pulmonary infections in patients with cystic fibrosis. Curr Opin Pulm Med 9:509–515 [CrossRef]
    [Google Scholar]
  7. Cieri M. V., Mayer-Hamblett N., Griffith A., Burns J. L. 2002; Correlation between an in vitro invasion assay and a murine model of Burkholderia cepacia lung infection. Infect Immun 70:1081–1086 [CrossRef]
    [Google Scholar]
  8. Coenye T., Falsen E., Hoste B., Ohlen M., Goris J., Govan J. R., Gillis M., Vandamme P. 2000; Description of Pandoraea gen. nov. with Pandoraea apista sp.nov., Pandoraeapulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp.nov. and Pandoraea norimbergensis comb. nov. Int J Syst Evol Microbiol 50:887–899 [CrossRef]
    [Google Scholar]
  9. Coenye T., Liu L., Vandamme P., LiPuma J. J. 2001; Identification of Pandoraea species by 16S ribosomal DNA-based PCR assays. J Clin Microbiol 39:4452–4455 [CrossRef]
    [Google Scholar]
  10. Daneshvar M. I., Hollis D. G., Steigerwalt A. G., Whitney A. M., Spangler L., Douglas M. P., Jordan J. G., MacGregor J. P., Hill B. C. other authors 2001; Assignment of CDC weak oxidizer group 2 (WO-2) to the genus Pandoraea and characterization of three new Pandoraea genomospecies. J Clin Microbiol 39:1819–1826 [CrossRef]
    [Google Scholar]
  11. De Rose V. 2002; Mechanisms and markers of airway inflammation in cystic fibrosis. Eur Respir J 19:333–340 [CrossRef]
    [Google Scholar]
  12. Desai M., Buhler T., Weller P. H., Brown M. R. 1998; Increasing resistance of planktonic and biofilm cultures of Burkholderia cepacia to ciprofloxacin and ceftazidime during exponential growth. J Antimicrob Chemother 42:153–160 [CrossRef]
    [Google Scholar]
  13. Duff C., Murphy P. G., Callaghan M., McClean S. 2006; Differences in invasion and translocation of Burkholderia cepacia complex species in polarised lung epithelial cells in vitro. Microb Pathog 41:183–192 [CrossRef]
    [Google Scholar]
  14. Gibson R. L., Burns J. L., Ramsey B. W. 2003; Pathophysiology and management of pulmonary infections in cystic fibrosis. Am J Respir Crit Care Med 168:918–951 [CrossRef]
    [Google Scholar]
  15. Hoiby N., Krogh Johansen H., Moser C., Song Z., Ciofu O., Kharazmi A. 2001; Pseudomonas aeruginosa and the in vitro and in vivo biofilm mode of growth. Microbes Infect 3:23–35 [CrossRef]
    [Google Scholar]
  16. Johnson L. N., Han J. Y., Moskowitz S. M., Burns J. L., Qin X., Englund J. A. 2004; Pandoraea bacteremia in a cystic fibrosis patient with associated systemic illness. Pediatr Infect Dis J 23:881–882 [CrossRef]
    [Google Scholar]
  17. Jorgensen I. M., Johansen H. K., Frederiksen B., Pressler T., Hansen A., Vandamme P., Hoiby N., Koch C. 2003; Epidemic spread of Pandoraea apista , a new pathogen causing severe lung disease in cystic fibrosis patients. Pediatr Pulmonol 36:439–446 [CrossRef]
    [Google Scholar]
  18. Khair O. A., Davies R. J., Devalia J. L. 1996; Bacterial-induced release of inflammatory mediators by bronchial epithelial cells. Eur Respir J 9:1913–1922 [CrossRef]
    [Google Scholar]
  19. Khan T. Z., Wagener J. S., Bost T., Martinez J., Accurso F. J., Riches D. W. 1995; Early pulmonary inflammation in infants with cystic fibrosis. Am J Respir Crit Care Med 151:1075–1082
    [Google Scholar]
  20. Martin D. W., Mohr C. D. 2000; Invasion and intracellular survival of Burkholderia cepacia . Infect Immun 68:24–29 [CrossRef]
    [Google Scholar]
  21. Moskowitz S. M., Foster J. M., Emerson J., Burns J. L. 2004; Clinically feasible biofilm susceptibility assay for isolates of Pseudomonas aeruginosa from patients with cystic fibrosis. J Clin Microbiol 42:1915–1922 [CrossRef]
    [Google Scholar]
  22. Muhlebach M. S., Noah T. L. 2002; Endotoxin activity and inflammatory markers in the airways of young patients with cystic fibrosis. Am J Respir Crit Care Med 165:911–915 [CrossRef]
    [Google Scholar]
  23. Noah T. L., Black H. R., Cheng P. W., Wood R. E., Leigh M. W. 1997; Nasal and bronchoalveolar lavage fluid cytokines in early cystic fibrosis. J Infect Dis 175:638–647 [CrossRef]
    [Google Scholar]
  24. Palfreyman R. W., Watson M. L., Eden C., Smith A. W. 1997; Induction of biologically active interleukin-8 from lung epithelial cells by Burkholderia ( Pseudomonas ) cepacia products. Infect Immun 65:617–622
    [Google Scholar]
  25. Stryjewski M. E., LiPuma J. J., Messier R. H. Jr, Reller L. B., Alexander B. D. 2003; Sepsis, multiple organ failure and death due to Pandoraea pnomenusa infection after lung transplantation. J Clin Microbiol 41:2255–2257 [CrossRef]
    [Google Scholar]
  26. Yu H., Head N. E. 2002; Persistent infections and immunity in cystic fibrosis. Front Biosci 7:d442–457
    [Google Scholar]
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