1887

Abstract

, and spp. are phenotypically and genetically closely related pathogens that may cause disease with similar clinical presentation. Consequently, difficulties in their identification and differentiation have been reported. In this study, a sensitive gene-based multi-primer single-target PCR (MP-ST-PCR) was developed that allowed the specific detection and differentiation of these clinically relevant pathogens. The specificity of the assay was evaluated using a representative panel of 50 and 16 strains and the type strains of all spp. Detection limits for purified DNA from , and were 100, 10 and 100 fg, respectively. DNA was also successfully detected in various clinical specimens from a human patient with culture-proven brucellosis and from a -infected sheep and its aborted fetuses. The sensitivity of the MP-ST-PCR was comparable to that of an evaluated in-house real-time PCR assay. The developed assay closes a diagnostic gap and provides a simple but robust tool for the sensitive detection and correct identification of , and spp.

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2008-01-01
2019-10-15
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