1887

Abstract

To assess the prevalence of enterotoxigenic among adults suffering from antibiotic-associated diarrhoea in a Costa Rican hospital, faecal samples were analysed from 104 patients by a cultivation approach. The 29 strains obtained, which accounted for an isolation frequency of 28 %, were genotyped and investigated with regard to their susceptibility to penicillin, imipenem, cefotaxime, chloramphenicol and metronidazole using an agar-dilution method. A multiplex PCR for detection of the toxins , and ϵ predictably classified all faecal isolates as biotype A. An agglutination assay revealed that only one isolate synthesized detectable amounts of enterotoxin (detection rate 3 %). This result was confirmed by a PCR targeting the gene. The spores of the only CPE isolate did not germinate after incubation for 30 min at temperatures above 80 °C. Most isolates were susceptible to first-choice antimicrobials. However, unusual MICs for penicillin (16 μg ml) and metronidazole (512 μg ml) were detected in one and three isolates, respectively. The low incidence of enterotoxigenic strains suggests that was not a major primary cause of antibiotic-associated diarrhoea in this hospital during the sampling period.

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2008-03-01
2019-09-17
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