1887

Abstract

The obligate intracellular pathogen is transmitted by ticks and causes human granulocytic anaplasmosis, tick-borne fever of ruminants, and equine and canine granulocytic anaplasmosis. In a previous study, the perilipin (PLIN) gene was identified as one of the genes differentially expressed in human promyelocytic HL-60 cells in response to infection with . PLIN is a major adipocyte lipid droplet-associated phosphoprotein that plays a central role in lipolysis and cholesterol synthesis. Host cholesterol and other lipids are required by for infection and multiplication in human cells. In this study, it was hypothesized that PLIN may be involved in infection of human HL-60 cells by . To test this hypothesis, a combination of real-time RT-PCR, immunofluorescence and RNA interference was used to study the expression of PLIN. The results of these studies demonstrated that modulates lipid metabolism by increasing PLIN mRNA levels and facilitates infection of HL-60 cells. The results of these studies expand our knowledge of the role of lipid metabolism in infection and multiplication in HL-60 cells and suggest a mechanism by which modulates lipid metabolism.

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2008-02-01
2024-12-07
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References

  1. Carlyon J. A., Fikrig E. 2003; Invasion and survival strategies of Anaplasma phagocytophilum . Cell Microbiol 5:743–754 [CrossRef]
    [Google Scholar]
  2. de la Fuente J., Massung R. F., Wong S. J., Chu F. K., Lutz H., Meli M., von Loewenich F. D., Grzeszczuk A., Torina A. other authors 2005a; Sequence analysis of the msp4 gene of Anaplasma phagocytophilum strains. J Clin Microbiol 43:1309–1317 [CrossRef]
    [Google Scholar]
  3. de la Fuente J., Ayoubi P., Blouin E. F., Almazán C., Naranjo V., Kocan K. M. 2005b; Gene expression profiling of human promyelocytic cells in response to infection with Anaplasma phagocytophilum . Cell Microbiol 7:549–559
    [Google Scholar]
  4. de la Fuente J., Almazán C., Blouin E. F., Naranjo V., Kocan K. M. 2006; Reduction of tick infections with Anaplasma marginale and A. phagocytophilum by targeting the tick protective antigen subolesin. Parasitol Res 100:85–91 [CrossRef]
    [Google Scholar]
  5. Dumler J. S., Barbet A. C., Bekker C. P. J., Dasch G. A., Palmer G. H., Ray S. C., Rikihisa Y., Rurangirwa F. R. 2001; Reorganization of the genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales : unification of some species of Ehrlichia with Anaplasma , Cowdria with Ehrlichia and Ehrlichia with Neorickettsia , descriptions of six new species combinations and designation of Ehrlichia equi and ‘HGE agent’ as subjective synonyms of Ehrlichia phagocytophila . Int J Syst Evol Microbiol 51:2145–2165 [CrossRef]
    [Google Scholar]
  6. Dumler J. S., Choi K. S., Garcia-Garcia J. C., Barat N. S., Scorpio D. G., Garyu J. W., Grab D. J., Bakken J. S. 2005; Human granulocytic anaplasmosis and Anaplasma phagocytophilum . Emerg Infect Dis 11:1828–1834 [CrossRef]
    [Google Scholar]
  7. Granneman J. G., Moore H. P., Granneman R. L., Greenberg A. S., Obin M. S., Zhu Z. 2007; Analysis of lipolytic protein trafficking and interactions in adipocytes. J Biol Chem 282:5726–5735 [CrossRef]
    [Google Scholar]
  8. Lin M., Rikihisa Y. 2003a; Obligatory intracellular parasitism by Ehrlichia chaffeensis and Anaplasma phagocytophilum involves caveolae and glycosylphosphatidylinositol-anchored proteins. Cell Microbiol 5:809–820 [CrossRef]
    [Google Scholar]
  9. Lin M., Rikihisa Y. 2003b; Ehrlichia chaffeensis and Anaplasma phagocytophilum lack genes for lipid A biosynthesis and incorporate cholesterol for their survival. Infect Immun 71:5324–5331 [CrossRef]
    [Google Scholar]
  10. Miyoshi H., Souza S. C., Zhang H. H., Strissel K. J., Christoffolete M. A., Kovsan J., Rudich A., Kraemer F. B., Bianco A. C. other authors 2006; Perilipin promotes hormone-sensitive lipase-mediated adipocyte lipolysis via phosphorylation-dependent and -independent mechanisms. J Biol Chem 281:15837–15844 [CrossRef]
    [Google Scholar]
  11. Moore H. P., Silver R. B., Mottillo E. P., Bernlohr D. A., Granneman J. G. 2005; Perilipin targets a novel pool of lipid droplets for lipolytic attack by hormone-sensitive lipase. J Biol Chem 280:43109–43120 [CrossRef]
    [Google Scholar]
  12. Nicklas J. A., Buel E. 2003; Development of an Alu-based, real-time PCR method for quantitation of human DNA in forensic samples. J Forensic Sci 48:936–944
    [Google Scholar]
  13. Pedra J. H., Sukumaran B., Carlyon J. A., Berliner N., Fikrig E. 2005; Modulation of NB4 promyelocytic leukemic cell machinery by Anaplasma phagocytophilum . Genomics 86:365–377 [CrossRef]
    [Google Scholar]
  14. Sukumaran B., Carlyon J. A., Cai J. L., Berliner N., Fikrig E. 2005; Early transcriptional response of human neutrophils to Anaplasma phagocytophilum infection. Infect Immun 73:8089–8099 [CrossRef]
    [Google Scholar]
  15. Thomas V., Fikrig E. 2007; Anaplasma phagocytophilum specifically induces tyrosine phosphorylation of ROCK1 during infection. Cell Microbiol 9:1730–1737 [CrossRef]
    [Google Scholar]
  16. Xiong Q., Wang X., Rikihisa Y. 2007; High-cholesterol diet facilitates Anaplasma phagocytophilum infection and up-regulates macrophage inflammatory protein-2 and CXCR2 expression in apolipoprotein E-deficient mice. J Infect Dis 195:1497–1503 [CrossRef]
    [Google Scholar]
  17. Yeaman S. J. 2004; Hormone-sensitive lipase – new roles for an old enzyme. Biochem J 379:11–22 [CrossRef]
    [Google Scholar]
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