1887

Abstract

The aim of this study was to compare conventional 16S rRNA gene PCR, real-time 16S rRNA gene PCR and real-time adhesin protein (MgPa) gene PCR as detection methods for infection. The study also determined the prevalence of in male and female patients attending a sexually transmitted infections clinic in a rural area in the west of Sweden. First void urine (FVU) and/or urethral swabs were collected from 381 men, and FVU and/or cervical swabs and/or urethral swabs were collected from 298 women. A total of 213 specimens were used in the PCR comparative study: 98 consecutively sampled specimens from patients enrolled in the prevalence study, 36 consecutively sampled specimens from patients with symptoms of urethritis and 79 specimens from patients positive for by real-time MgPa gene PCR in the prevalence study. A true-positive DNA specimen was defined as either a specimen positive in any two PCR assays or a specimen whose PCR product was verified by DNA sequencing. The prevalence of infection in men and women was 27/381 (7.1 %) and 23/298 (7.7 %), respectively. In the PCR comparative study, DNA was detected in 61/76 (80.3 %) of true-positive specimens by conventional 16S rRNA gene PCR, in 52/76 (68.4 %) by real-time 16S rRNA gene PCR and in 74/76 (97.4 %) by real-time MgPa gene PCR. Real-time MgPa gene PCR thus had higher sensitivity compared with conventional 16S rRNA gene PCR and had considerably increased sensitivity compared with real-time 16S rRNA gene PCR for detection of DNA. Real-time MgPa gene PCR is well suited for the clinical diagnosis of .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47498-0
2008-03-01
2020-07-12
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/3/304.html?itemId=/content/journal/jmm/10.1099/jmm.0.47498-0&mimeType=html&fmt=ahah

References

  1. Anagrius C., Lore B., Jensen J. S. 2005; Mycoplasma genitalium : prevalence, clinical significance, and transmission. Sex Transm Infect 81:458–462 [CrossRef]
    [Google Scholar]
  2. Björnelius E., Lidbrink P., Jensen J. S. 2000; Mycoplasma genitalium in non-gonococcal urethritis – a study in Swedish male STD patients. Int J STD AIDS 11:292–296 [CrossRef]
    [Google Scholar]
  3. Cohen C. R., Manhart L. E., Bukusi E. A., Astete S., Brunham R. C., Holmes K. K., Sinei S. K., Bwayo J. J., Totten P. A. 2002; Association between Mycoplasma genitalium and acute endometritis. Lancet 359:765–766 [CrossRef]
    [Google Scholar]
  4. Deguchi T., Maeda S. 2002; Mycoplasma genitalium : another important pathogen of nongonococcal urethritis. J Urol 167:1210–1217 [CrossRef]
    [Google Scholar]
  5. Falk L., Fredlund H., Jensen J. S. 2004; Symptomatic urethritis is more prevalent in men infected with Mycoplasma genitalium than with Chlamydia trachomatis . Sex Transm Infect 80:289–293 [CrossRef]
    [Google Scholar]
  6. Falk L., Fredlund H., Jensen J. S. 2005; Signs and symptoms of urethritis and cervicitis among women with or without Mycoplasma genitalium or Chlamydia trachomatis infection. Sex Transm Infect 81:73–78 [CrossRef]
    [Google Scholar]
  7. Gambini D., Decleva I., Lupica L., Ghislanzoni M., Cusini M., Alessi E. 2000; Mycoplasma genitalium in males with nongonococcal urethritis: prevalence and clinical efficacy of eradication. Sex Transm Dis 27:226–229 [CrossRef]
    [Google Scholar]
  8. Jensen J. S. 2004; Mycoplasma genitalium : the aetiological agent of urethritis and other sexually transmitted diseases. J Eur Acad Dermatol Venereol 18:1–11
    [Google Scholar]
  9. Jensen J. S. 2006; Mycoplasma genitalium infections. Diagnosis, clinical aspects, and pathogenesis. Dan Med Bull 53:1–27
    [Google Scholar]
  10. Jensen J. S., Uldum S. A., Søndergård-Andersen J., Vuust J., Lind K. 1991; Polymerase chain reaction for detection of Mycoplasma genitalium in clinical samples. J Clin Microbiol 29:46–50
    [Google Scholar]
  11. Jensen J. S., Borre M. B., Dohn B. 2003; Detection of Mycoplasma genitalium by PCR amplification of the 16S rRNA gene. J Clin Microbiol 41:261–266 [CrossRef]
    [Google Scholar]
  12. Jensen J. S., Björnelius E., Dohn B., Lidbrink P. 2004a; Comparison of first void urine and urogenital swab specimens for detection of Mycoplasma genitalium and Chlamydia trachomatis by polymerase chain reaction in patients attending a sexually transmitted disease clinic. Sex Transm Dis 31:499–507 [CrossRef]
    [Google Scholar]
  13. Jensen J. S., Björnelius E., Dohn B., Lidbrink P. 2004b; Use of TaqMan 5′ nuclease real-time PCR for quantitative detection of Mycoplasma genitalium DNA in males with and without urethritis who were attendees at a sexually transmitted disease clinic. J Clin Microbiol 42:683–692 [CrossRef]
    [Google Scholar]
  14. Johannisson G., Enström Y., Löwhagen G. B., Nagy V., Ryberg K., Seeberg S., Welinder-Olsson C. 2000; Occurrence and treatment of Mycoplasma genitalium in patients visiting STD clinics in Sweden. Int J STD AIDS 11:324–326 [CrossRef]
    [Google Scholar]
  15. Jurstrand M., Jensen J. S., Fredlund H., Falk L., Mölling P. 2005; Detection of Mycoplasma genitalium in urogenital specimens by real-time PCR and by conventional PCR assay. J Med Microbiol 54:23–29 [CrossRef]
    [Google Scholar]
  16. Lind K., Lindhardt B. Ø., Schütten H. J., Blom J., Christiansen C. 1984; Serological cross-reactions between Mycoplasma genitalium and Mycoplasma pneumoniae . J Clin Microbiol 20:1036–1043
    [Google Scholar]
  17. Martinelli F., Garrafa E., Turano A., Caruso A. 1999; Increased frequency of detection of Ureaplasma urealyticum and Mycoplasma genitalium in AIDS patients without urethral symptoms. J Clin Microbiol 37:2042–2044
    [Google Scholar]
  18. Mellenius H., Boman J., Lundqvist E. N., Jensen J. S. 2005; Mycoplasma genitalium should be suspected in unspecific urethritis and cervicitis. A study from Vasterbotten confirms the high prevalence of the bacteria. Lakartidningen 102: 35383540–3541
    [Google Scholar]
  19. Svenstrup H. F., Jensen J. S., Björnelius E., Lidbrink P., Birkelund S., Christiansen G. 2005; Development of a quantitative real-time PCR assay for detection of Mycoplasma genitalium . J Clin Microbiol 43:3121–3128 [CrossRef]
    [Google Scholar]
  20. Taylor-Robinson D. 2002; Mycoplasma genitalium – an up-date. Int J STD AIDS 13:145–151 [CrossRef]
    [Google Scholar]
  21. Taylor-Robinson D., Furr P. M., Tully J. G. 1983; Serological cross-reactions between Mycoplasma genitalium and M. pneumoniae . Lancet 1:527
    [Google Scholar]
  22. Tully J. G., Taylor-Robinson D., Cole R. M., Rose D. L. 1981; A newly discovered mycoplasma in the human urogenital tract. Lancet 1:1288–1291
    [Google Scholar]
  23. Uusküla A., Kohl P. K. 2002; Genital mycoplasmas, including Mycoplasma genitalium , as sexually transmitted agents. Int J STD AIDS 13:79–85 [CrossRef]
    [Google Scholar]
  24. Yoshida T., Deguchi T., Ito M., Maeda S., Tamaki M., Ishiko H. 2002; Quantitative detection of Mycoplasma genitalium from first-pass urine of men with urethritis and asymptomatic men by real-time PCR. J Clin Microbiol 40:1451–1455 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47498-0
Loading
/content/journal/jmm/10.1099/jmm.0.47498-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error