1887

Abstract

This pilot study examined the change in the seroprevalence of the enteroaggregative (EAEC) virulence factor dispersin in USA students during a short stay in Cuernavaca, Mexico, between June and August 2004. One hundred and ninety-five students provided paired serum samples – one on arrival to Mexico (pre-serum) and a second on departure from Mexico (post-serum) after a mean stay of 19 days. Serum samples were tested for IgG antibody to a recombinant purified dispersin protein by ELISA. For all travellers, with and without diarrhoea, the mean± pre-serum absorbance value (read at 450 and 570 nm) was 0.340±0.212 and the mean post-serum value was 0.513±0.316 (<0.00001). Both travellers who developed diarrhoea and those who did not develop diarrhoea had an increase in IgG antibody to dispersin from the time of arrival to the time of departure from Cuernavaca (diarrhoea group 0.323±0.197 to 0.501±0.311, <0.00001, and the asymptomatic group 0.354±0.224 to 0.525±0.321, <0.00001). The pre-serum absorbance value (read at 450 and 570 nm) for IgG antibody to dispersin was not associated with protection against the development of diarrhoeal illness. These results indicate that USA travellers to Mexico show seroconversion for the EAEC virulence factor dispersin. Further studies are needed to characterize in more detail the host clinical and immunological responses to the dispersin protein.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47495-0
2008-04-01
2019-11-12
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/4/476.html?itemId=/content/journal/jmm/10.1099/jmm.0.47495-0&mimeType=html&fmt=ahah

References

  1. Bendtsen, J. D., Nielsen, H., von Heijne, G. & Brunak, S. ( 2004; ). Improved prediction of signal peptides: Signal P 3.0. J Mol Biol 340, 783–795.[CrossRef]
    [Google Scholar]
  2. Brown, E. L., Reisenbichler, E. S., Kim, J. H. & Höök, M. ( 2005; ). Multicomponent Lyme vaccine: three is not a crowd. Vaccine 23, 3687–3696.[CrossRef]
    [Google Scholar]
  3. Donnenberg, M. S. & Nataro, J. P. ( 1995; ). Methods for studying adhesion of diarrheagenic Escherichia coli. Methods Enzymol 253, 324–336.
    [Google Scholar]
  4. Nataro, J. P. ( 2005; ). Enteroaggregative Escherichia coli pathogenesis. Curr Opin Gastroenterol 21, 4–8.
    [Google Scholar]
  5. Nataro, J. P., Deng, Y., Cookson, S., Cravioto, A., Savarino, S. J., Guers, L. D., Levine, M. M. & Tacket, C. O. ( 1995; ). Heterogeneity of enteroaggregative Escherichia coli virulence demonstrated in volunteers. J Infect Dis 171, 465–468.[CrossRef]
    [Google Scholar]
  6. Nishi, J., Sheikh, J., Mizuguchi, K., Luisi, B., Burland, V., Boutin, A., Rose, D. J., Blattner, F. R. & Nataro, J. P. ( 2003; ). The export of coat protein from enteroaggregative Escherichia coli by a specific ATP-binding cassette transporter system. J Biol Chem 278, 45680–45689.[CrossRef]
    [Google Scholar]
  7. Sheikh, J., Czeczulin, J. R., Harrington, S., Hicks, S., Henderson, I. R., Le Bouguenec, C., Gounon, P., Phillips, A. & Nataro, J. P. ( 2002; ). A novel dispersin protein in enteroaggregative Escherichia coli. J Clin Invest 110, 1329–1337.[CrossRef]
    [Google Scholar]
  8. Steffen, R. ( 2005; ). Epidemiology of traveler's diarrhea. Clin Infect Dis 41, S536–S540.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47495-0
Loading
/content/journal/jmm/10.1099/jmm.0.47495-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error