1887

Abstract

Immunofluorescence microscopy-based identification of presumptive isolates, using the -specific mAb QUBPa3, revealed five organisms with an atypical cellular morphology. Unlike the coryneform morphology seen with types I and II, these isolates exhibited long slender filaments (which formed large tangled aggregates) not previously described in . No reaction with mAbs that label types IA (QUBPa1) and II (QUBPa2) was observed. Nucleotide sequencing of the 16S rRNA gene (1484 bp) revealed the isolates to have between 99.8 and 99.9 % identity to the 16S rRNA gene of the type IA, IB and II strains NCTC 737, KPA171202 and NCTC 10390, respectively. Analysis of the housekeeping gene (1047 bp) did reveal, however, a greater number of conserved nucleotide polymorphisms between the sequences from these isolates and those from NCTC 737 (98.9 % identity), KPA171202 (98.9 % identity) and NCTC 10390 (99.1 % identity). Phylogenetic investigations demonstrated that the isolates belong to a novel cluster or lineage distinct from types I and II. We now propose this new grouping as type III. The prevalence and clinical importance of this novel lineage amongst isolates of remains to be determined.

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2008-02-01
2020-04-07
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References

  1. Ahn C. Y., Ko C. Y., Wagar E. A., Wong R. S., Shaw W. W. 1996; Microbial evaluation: 139 implants removed from symptomatic patients. Plast Reconstr Surg 98:1225–1229 [CrossRef]
    [Google Scholar]
  2. Aldave A. J., Stein J. D., Deramo V. A., Shah G. K., Fischer D. H., Maguire J. I. 1999; Treatment strategies for postoperative Propionibacterium acnes endophthalmitis. Ophthalmology 106:2395–2401 [CrossRef]
    [Google Scholar]
  3. Balke E., Weiss R. 1984; A simple method for rapid detection of bacterial hyaluronidase in K hyaluronate-containing gel. Zentralbl Bakteriol Mikrobiol Hyg [A] 257:317–322 (in German
    [Google Scholar]
  4. Brook I., Frazier E. H. 1991; Infections caused by Propionibacterium species. Rev Infect Dis 13:819–822 [CrossRef]
    [Google Scholar]
  5. Bruggemann H., Henne A., Hoster F., Liesegang H., Wiezer A., Strittmatter A., Hujer S., Durre P., Gottschalk G. 2004; The complete genome sequence of Propionibacterium acnes , a commensal of human skin. Science 305:671–673 [CrossRef]
    [Google Scholar]
  6. Clark W. L., Kaiser P. K., Flynn H. W., Belfort A., Miller D., Meisler D. M. 1999; Treatment strategies and visual acuity outcomes in chronic postoperative Propionibacterium acnes endophthalmitis. Ophthalmology 106:1665–1670 [CrossRef]
    [Google Scholar]
  7. Cohan F. M. 2002; What are bacterial species?. Annu Rev Microbiol 56:457–487 [CrossRef]
    [Google Scholar]
  8. Cohen R. J., Shannon B. A., McNeal J. E., Shannon T., Garrett K. L. 2005; Propionibacterium acnes associated with inflammation in radical prostatectomy specimens: a possible link to cancer evolution?. J Urol 173:1969–1974 [CrossRef]
    [Google Scholar]
  9. Debelian G. J., Olsen I., Tronstad L. 1992; Profiling of Propionibacterium acnes recovered from root canal and blood during and after endodontic treatment. Endod Dent Traumatol 8:248–254 [CrossRef]
    [Google Scholar]
  10. Dekio I., Hayashi H., Sakamoto M., Kitahara M., Nishikawa T., Suematsu M., Benno Y. 2005; Detection of potentially novel bacterial components of the human skin microbiota using culture-independent molecular profiling. J Med Microbiol 54:1231–1238 [CrossRef]
    [Google Scholar]
  11. Delahaye F., Fol S., Celard M., Vandenesch F., Beaune J., Bozio A., de Gevigney G. 2005; Propionibacterium acnes infective endocarditis. Study of 11 cases and review of literature. Arch Mal Coeur Vaiss 98:1212–1218 (in French
    [Google Scholar]
  12. Eady E. A., Ingham E. 1994; Propionibacterium acnes – friend or foe?. Rev Med Microbiol 5:163–173 [CrossRef]
    [Google Scholar]
  13. Eisen J. A. 1995; The RecA protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of RecAs and 16S rRNAs from the same species. J Mol Evol 41:1105–1123
    [Google Scholar]
  14. Eishi Y., Suga M., Ishige I., Kobayashi D., Yamada T., Takemura T., Takizawa T., Koike M., Kudoh S. other authors 2002; Quantitative analysis of mycobacterial and propionibacterial DNA in lymph nodes of Japanese and European patients with sarcoidosis. J Clin Microbiol 40:198–204 [CrossRef]
    [Google Scholar]
  15. Hall V. 2006; Anaerobic actinomycetes and related organisms. In Principles and Practice of Clinical Bacteriology pp 575–586 Edited by Gillespie S. H., Hawkey P. M. Chichester: Wiley;
    [Google Scholar]
  16. Higaki S., Kitagawa T., Kagoura M., Morohashi M., Yamagishi T. 2000; Correlation between Propionibacterium acnes biotypes, lipase activity and rash degree in acne patients. J Dermatol 27:519–522 [CrossRef]
    [Google Scholar]
  17. Holt J. G., Krieg N. R., Sneath P. H. A., Staley J. T., Williams S. T. (editors) 1994 Bergey's Manual of Determinative Bacteriology , 9th edn. Baltimore, MD: Williams and Wilkins;
    [Google Scholar]
  18. Horgan S. E., Matheson M. M., McLoughlin-Borlace L., Dart J. K. 1999; Use of a low nutrient culture medium for the identification of bacteria causing severe ocular infection. J Med Microbiol 48:701–703 [CrossRef]
    [Google Scholar]
  19. Ishii S., Meyer K. P., Sadowsky M. J. 2007; Relationship between phylogenetic groups, genotypic clusters and virulence gene profiles of Escherichia coli strains from diverse human and animal sources. Appl Environ Microbiol 73:5703–5710 [CrossRef]
    [Google Scholar]
  20. Johnson J. L., Cummins C. S. 1972; Cell wall composition and deoxyribonucleic acid similarities among anaerobic coryneforms, classical propionibacteria, and strains of Arachnia propionica . J Bacteriol 109:1047–1066
    [Google Scholar]
  21. Kishishita M., Ushijima T., Ozaki Y., Ito Y. 1979; Biotyping of Propionibacterium acnes isolated from normal human facial skin. Appl Environ Microbiol 38:585–589
    [Google Scholar]
  22. Kotilainen P., Merilahti-Palo R., Lehtonen O. P., Manner I., Helander I., Mottonen T., Rintala E. 1996; Propionibacterium acnes isolated from sternal osteitis in a patient with SAPHO syndrome. J Rheumatol 23:1302–1304
    [Google Scholar]
  23. Le Goff A., Bunetel L., Mouton C., Bonnaure-Mallet M. 1997; Evaluation of root canal bacteria and their antimicrobial susceptibility in teeth with necrotic pulp. Oral Microbiol Immunol 12:318–322 [CrossRef]
    [Google Scholar]
  24. LiPuma J. J., Dulaney B. J., McMenamin J. D., Whitby P. W., Stull T. L., Coenye T., Vandamme P. 1999; Development of rRNA-based PCR assays for identification of Burkholderia cepacia complex isolates recovered from cystic fibrosis patients. J Clin Microbiol 37:3167–3170
    [Google Scholar]
  25. Mahenthiralingam E., Bischof J., Byrne S. K., Radomski C., Davies J. E., Av-Gay Y., Vandamme P. 2000; DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis , and Burkholderia cepacia genomovars I and III. J Clin Microbiol 38:3165–3173
    [Google Scholar]
  26. McDowell A., Valanne S., Ramage G., Tunney M. M., Glenn J. V., McLorinan G. C., Bhatia A., Maisonneuve J. F., Lodes M. other authors 2005; Propionibacterium acnes types I and II represent phylogenetically distinct groups. J Clin Microbiol 43:326–334 [CrossRef]
    [Google Scholar]
  27. McGinley K. J., Webster G. F., Leyden J. J. 1978; Regional variations of cutaneous propionibacteria. Appl Environ Microbiol 35:62–66
    [Google Scholar]
  28. Palys T., Nakamura L. K., Cohan F. M. 1997; Discovery and classification of ecological diversity in the bacterial world: the role of DNA sequence data. Int J Syst Bacteriol 47:1145–1156 [CrossRef]
    [Google Scholar]
  29. Perry A. L., Worthington T., Hilton A. C., Lambert P. A., Stirling A. J., Elliott T. S. J. 2003; Analysis of clinical isolates of Propionibacterium acnes by optimised RAPD. FEMS Microbiol Lett 228:51–55 [CrossRef]
    [Google Scholar]
  30. Spare M. K., Tebbs S. E., Lang S., Lambert P. A., Worthington T., Lipkin G. W., Elliott T. S. J. 2003; Genotypic and phenotypic properties of coagulase negative staphylococci causing dialysis catheter related sepsis. J Hosp Infect 54:272–278 [CrossRef]
    [Google Scholar]
  31. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  32. Tunney M. M., Patrick S., Gorman S. P., Nixon J. R., Anderson N., Davis R. I., Hanna D., Ramage G. 1998; Improved detection of infection in hip replacements. J Bone Joint Surg Br 80:568–572 [CrossRef]
    [Google Scholar]
  33. Tunney M. M., Patrick S., Curran M. D., Ramage G., Hanna D., Nixon J. R., Gorman S. P., Davis R. I., Anderson N. 1999; Detection of prosthetic hip infection at revision arthroplasty by immunofluorescence microscopy and PCR amplification of the bacterial 16S rRNA gene. J Clin Microbiol 37:3281–3290
    [Google Scholar]
  34. Valanne S., McDowell A., Ramage G., Tunney M. M., Einarsson G. G., O'Hagan S., Wisdom G. B., Fairley D., Bhatia A. other authors 2005; CAMP factor homologues in Propionibacterium acnes : a new protein family differentially expressed by types I and II. Microbiology 151:1369–1379 [CrossRef]
    [Google Scholar]
  35. Vandamme P., Pot B., Gillis M., Devos P., Kersters K., Swings J. 1996; Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60:407–438
    [Google Scholar]
  36. Webster G. F., Cummins C. S. 1978; Use of bacteriophage typing to distinguish Propionibacterium acnes types I and II. J Clin Microbiol 7:84–90
    [Google Scholar]
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