1887

Abstract

The genome contains four copies of an operon called (). Previously we reported that disrupted in the operon is more virulent than wild-type in mice. We generated single deletion mutants in ) and ) operons and a double deletion mutant (Δ/). Similar doubling times and growth characteristics were observed for all mutants and the wild-type (parent) H37Rv strain in culture and in macrophages. In addition, similar bacterial burdens were detected in organs from mice infected with Δ and the parent strain. However, the bacterial burdens of mice infected with Δ and Δ/ were less than those of mice infected with the parent strain. The median survival times of mice infected with wild-type , Δ, Δ and Δ/ were 40.5, 46, 58 and 62 weeks, respectively. Histopathological examination of lungs at 15 weeks post-infection showed that the extent of the lung lesions was less prominent in mice infected with Δ and Δ/ mutants than in mice infected with the other two strains. These observations suggest that the and operons have a role distinct from that of for survival of .

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2008-02-01
2019-09-15
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