1887

Abstract

A rather fast and complicated progression of an infection caused by some strains of could be associated with the expression and co-action of virulence factor complexes in these strains. This study screened the antibiotic susceptibility and prevalence of virulence markers in isolates of meticillin-resistant (MRSA) obtained from patients hospitalized at the University Hospital in Olomouc, Czech Republic. A total of 100 isolates was screened for 13 genes encoding extracellular virulence determinants (, , , , , , , , , , , and ) and for their distribution in sample types. Eighty-nine isolates were positive for at least one of the genes. Genes for , , and were not detected in any of the MRSA isolates. No statistically significant differences in the occurrence of the determinants studied among sample types were found.

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2008-04-01
2019-10-23
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References

  1. Arbuthnott, J. P. ( 1982; ). Bacterial cytolysins (membrane-damaging toxins). In Molecular Actions of Toxins and Viruses, pp. 107–129. Edited by P. Cohen & S. van Heyningen. Amsterdam: Elsevier.
  2. Becker, K., Friedrich, A. W., Lubritz, G., Weilert, M., Peters, G. & von Tiff, C. ( 2003; ). Prevalence of genes encoding pyrogenic toxin superantigens and exfoliative toxins among strains of Staphylococcus aureus isolated from blood and nasal specimens. J Clin Microbiol 41, 1434–1439.[CrossRef]
    [Google Scholar]
  3. Bhakdi, S. & Tranum-Jensen, J. ( 1991; ). Alpha-toxin of Staphylococcus aureus. Microbiol Rev 55, 733–751.
    [Google Scholar]
  4. Bohach, G. A., Fast, D. J., Nelson, R. D. & Schlievert, P. M. ( 1990; ). Staphylococcal and streptococcal pyrogenic toxins involved in toxic shock syndrome and related illnesses. Crit Rev Microbiol 17, 251–272.[CrossRef]
    [Google Scholar]
  5. Cavassini, M., Wenger, A., Jaton, K., Blanc, D. S. & Bille, J. ( 1999; ). Evaluation of MRSA-Screen, a simple anti-PBP 2a slide latex agglutination kit, for rapid detection of methicillin resistance in Staphylococcus aureus. J Clin Microbiol 37, 1591–1594.
    [Google Scholar]
  6. Chambers, H. F. ( 1997; ). Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin Microbiol Rev 10, 781–791.
    [Google Scholar]
  7. Cheung, A. L., Wolf, C., Yeaman, M. R. & Bayer, A. S. ( 1995; ). Insertional inactivation of a chromosomal locus that modulates expression of potential virulence determinants in Staphylococcus aureus. J Bacteriol 177, 3220–3226.
    [Google Scholar]
  8. CLSI ( 2007; ). Performance Standards for Antimicrobial Susceptibility Testing, 17th informational supplement, M100–S17. Wayne, PA: Clinical and Laboratory Standards Institute.
  9. De Azavedo, J. & Arbuthnott, J. P. ( 1981; ). Prevalence of epidermolytic toxin in clinical isolates of Staphylococcus aureus. J Med Microbiol 14, 341–344.[CrossRef]
    [Google Scholar]
  10. Elsner, P. & Hartman, A. A. ( 1988; ). Epidemiology of ETA- and ETB- producing staphylococci in dermatological patients. Zentralbl Bakteriol Mikrobiol Hyg A 268, 534
    [Google Scholar]
  11. Felten, A., Grandry, B., Lagrange, P. H. & Casin, I. ( 2002; ). Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): a disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test. J Clin Microbiol 40, 2766–2771.[CrossRef]
    [Google Scholar]
  12. Foster, J. T. ( 2002; ). Staphylococcus aureus: extracellular toxins. In Molecular Medical Microbiology, pp. 843–865. Edited by G. J. Boulnois, G. Griffin, C. E. Hormaeche, G. Keusch, M. M. Levine, H. Smith, P. H. Williams & M. Sussman. San Diego: Academic Press.
  13. Holmes, A., Ganner, M., McGuane, S., Pitt, T. L., Cookson, B. D. & Kearns, A. M. ( 2005; ). Staphylococcus aureus isolates carrying Panton-Valentine leucocidin genes in England and Wales: frequency, characterization and association with clinical disease. J Clin Microbiol 43, 2384–2390.[CrossRef]
    [Google Scholar]
  14. Issartel, B., Tristan, A., Lechevallier, S., Bruyere, F., Lina, G., Garin, B., Lacassin, F., Bes, M., Vandenesch, F. & Etienne, J. ( 2005; ). Frequent carriage of Panton-Valentine leucocidin genes by Staphylococcus aureus isolates from surgically drained abscesses. J Clin Microbiol 43, 3203–3207.[CrossRef]
    [Google Scholar]
  15. Jackson, M. P. & Iandolo, I. I. ( 1986; ). Sequence of the exfoliative toxin B gene of Staphylococcus aureus. J Bacteriol 167, 726–728.
    [Google Scholar]
  16. Jarraud, S., Peyrat, M. A., Lim, A., Tristan, A., Bes, M., Mougel, C., Etienne, J., Vandenesch, F., Bonneville, M. & Lina, G. ( 2001; ). A highly prevalent operon of enterotoxin gene forms a putative nursery of superantigens in Staphylococcus aureus. J Immunol 166, 669–677.[CrossRef]
    [Google Scholar]
  17. Johansson, A., Flock, J. I. & Svensson, O. ( 2001; ). Collagen and fibronectin binding in experimental staphylococcal osteomyelitis. Clin Orthop Relat Res 382, 241–246.[CrossRef]
    [Google Scholar]
  18. Jonsson, K., Signas, Ch., Muller, H. P. & Lindberg, M. ( 1991; ). Two different genes encode fibronectin binding proteins in Staphylococcus aureus: the complete nucleotide sequence and characterization of the second gene. Eur J Biochem 202, 1041–1048.[CrossRef]
    [Google Scholar]
  19. Josefsson, E., McCrea, K. W., Ní Eidhin, D., O'Connell, D., Cox, J., Höök, M. & Foster, T. J. ( 1998; ). Three new members of the serine-aspartate repeat protein multigene family of Staphylococcus aureus. Microbiology 144, 3387–3395.[CrossRef]
    [Google Scholar]
  20. Kobayashi, N., Wu, H., Kojima, K., Taniguchi, K., Urasawa, S., Uehara, N., Omizu, Y., Kishi, Y., Yagihashi, A. & Kurokawa, I. ( 1994; ). Detection of mecA, femA and femB genes in clinical strains of staphylococci using polymerase chain reaction. Epidemiol Infect 113, 259–266.[CrossRef]
    [Google Scholar]
  21. Kondo, I., Sakurai, S., Sarai, Y. & Fukati, S. ( 1975; ). Two serotypes of exfoliatin and their distribution in staphylococcal strains isolated from patients with scalded skin syndrome. J Clin Microbiol 1, 397–400.
    [Google Scholar]
  22. Layer, F., Ghebremedhin, B., König, W. & König, B. ( 2006; ). Heterogeneity of methicillin-susceptible Staphylococcus aureus strains at a German university hospital implicates the circulating-strain pool as a potential source of emerging methicillin-resistant S. aureus clones. J Clin Microbiol 44, 2179–2185.[CrossRef]
    [Google Scholar]
  23. Lee, C. Y., Schmidt, J. J., Johnson-Winegar, A. D., Spero, L. & Iandolo, J. J. ( 1987; ). Sequence determination and comparison of the exfoliative toxin A and toxin B genes from Staphylococcus aureus. J Bacteriol 169, 3904–3909.
    [Google Scholar]
  24. Lehn, N., Schaller, E., Wagner, H. & Krönke, M. ( 1995; ). Frequency of toxic shock syndrome toxin and enterotoxin-producing clinical isolates of Staphylococcus aureus. Eur J Clin Microbiol Infect Dis 14, 43–46.[CrossRef]
    [Google Scholar]
  25. Lina, G., Piemont, Y., Godail-Gamot, F., Bes, M., Peter, M. O., Gauduchon, V., Vandenesch, F. & Etienne, J. ( 1999; ). Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis 29, 1128–1132.[CrossRef]
    [Google Scholar]
  26. Loncarevic, S., Jørgensen, H. J., Løvseth, A., Mathisen, T. & Rørvik, L. M. ( 2005; ). Diversity of Staphylococcus aureus enterotoxin types within single samples of raw milk and raw milk products. J Appl Microbiol 98, 344–350.[CrossRef]
    [Google Scholar]
  27. Løvseth, A., Loncarevic, S. & Berdal, K. G. ( 2004; ). Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates. J Clin Microbiol 42, 3869–3872.[CrossRef]
    [Google Scholar]
  28. McDevitt, D., Nanavaty, T., House-Pompeo, K., Bell, E., Turner, N., McIntire, L., Foster, T. & Höök, M. ( 1997; ). Characterization of the interaction between the Staphylococcus aureus clumping factor (ClfA) and fibrinogen. Eur J Biochem 247, 416–424.[CrossRef]
    [Google Scholar]
  29. Mehrotra, M., Wang, G. & Johnson, W. M. ( 2000; ). Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J Clin Microbiol 38, 1032–1035.
    [Google Scholar]
  30. Mempel, M., Lina, G., Hojka, M., Schnopp, C., Seidl, H. P., Schafer, T., Ring, J., Vandenesch, F. & Abeck, D. ( 2003; ). High prevalence of superantigens associated with the egc locus in Staphylococcus aureus isolates from patients with atopic eczema. Eur J Clin Microbiol Infect Dis 22, 306–309.
    [Google Scholar]
  31. Monday, S. R. & Bohach, G. A. ( 1999; ). Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in staphylococcal isolates. J Clin Microbiol 37, 3411–3414.
    [Google Scholar]
  32. Murono, K., Fujita, K. & Yoshioka, H. ( 1988; ). Microbiological characteristics of exfoliative toxin-producing Staphylococcus aureus. Pediatr Infect Dis J 7, 313–315.[CrossRef]
    [Google Scholar]
  33. Ní Eidhin, D., Perkins, S., Francois, P., Vaudaux, P., Höök, M. & Foster, T. J. ( 1998; ). Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus. Mol Microbiol 30, 245–257.[CrossRef]
    [Google Scholar]
  34. Oliveira, D. C. & De Lencastre, H. ( 2002; ). Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 46, 2155–2161.[CrossRef]
    [Google Scholar]
  35. Opal, S. M., Johnson-Winegar, A. D. & Gross, A. S. ( 1988; ). Staphylococcal scalded skin syndrome in two immunocompetent adults caused by exfoliatin B-producing Staphylococcus aureus. J Clin Microbiol 26, 1283–1286.
    [Google Scholar]
  36. Pantůček, R., Götz, F., Doškař, J. & Rosypal, S. ( 1996; ). Genomic variability of Staphylococcus aureus and the other coagulase-positive Staphylococcus species estimated by macrorestriction analysis using pulsed-field gel electrophoresis. Int J Syst Bacteriol 46, 216–222.[CrossRef]
    [Google Scholar]
  37. Park, P. W., Rosenbloom, J., Abrams, W. R., Rosenbloom, J. & Mecham, R. P. ( 1996; ). Molecular cloning and expression of the gene for elastin-binding protein (ebpS) in Staphylococcus aureus. J Biol Chem 271, 15803–15809.[CrossRef]
    [Google Scholar]
  38. Patti, J. M., Allen, B. L., McGavin, M. J. & Höök, M. ( 1994; ). MSCRAMM-mediated adherence of microorganisms to host tissues. Annu Rev Microbiol 48, 585–617.[CrossRef]
    [Google Scholar]
  39. Peacock, S. J., Moore, C. E., Justice, A., Kantzanou, M., Story, L., Mackie, K., O'Neill, G. & Day, N. P. ( 2002; ). Virulent combinations of adhesin and toxin genes in natural populations of Staphylococcus aureus. Infect Immun 70, 4987–4996.[CrossRef]
    [Google Scholar]
  40. Piemont, Y. & Monteil, H. ( 1983; ). Mise en évidence par électrosynérèse des deux sérotypes d'exfoliatine produits par Staphylococcus aureus. Ann Microbiol (Paris) 134A, 169–175.
    [Google Scholar]
  41. Prevost, G., Cribier, B., Couppie, P., Petiau, P., Supersac, G., Finck-Barbancon, V., Monteil, H. & Piemont, Y. ( 1995; ). Panton-Valentine leucocidin and gamma-hemolysin from Staphylococcus aureus ATCC 49775 are encoded by distinct genetic loci and have different biological activities. Infect Immun 63, 4121–4129.
    [Google Scholar]
  42. Rich, M., Deighton, L. & Roberts, L. ( 2005; ). Clindamycin-resistance in methicillin-resistant Staphylococcus aureus isolated from animals. Vet Microbiol 111, 237–240.[CrossRef]
    [Google Scholar]
  43. Sauer, P., Gallo, J., Kesselová, M., Kolář, M. & Koukalová, D. ( 2005; ). Universal primers for detection of common bacterial pathogens causing prosthetic joint infection. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149, 285–288.[CrossRef]
    [Google Scholar]
  44. Speziale, P., Raucci, G., Visai, L., Switalski, L. M., Timpl, R. & Höök, M. ( 1986; ). Binding of collagen to Staphylococcus aureus Cowan 1. J Bacteriol 167, 77–81.
    [Google Scholar]
  45. Tung, H., Guss, B., Hellman, U., Persson, L., Rubin, K. & Ryden, C. ( 2000; ). A bone sialoprotein-binding protein from Staphylococcus aureus: a member of the staphylococcal Sdr family. Biochem J 345, 611–619.[CrossRef]
    [Google Scholar]
  46. Uchiyama, T., Tadakuma, T., Imanishi, K., Araake, M., Saito, S., Yan, X. J., Fujikawa, H., Igarashi, H. & Yamaura, N. ( 1989; ). Activation of murine T cells by toxic shock syndrome toxin-1. The toxin-binding structures expressed on murine accessory cells are MHC class II molecules. J Immunol 143, 3175–3182.
    [Google Scholar]
  47. Wannet, W. J. B., Spalburg, E., Heck, M. E. O. C., Pluister, G. N., Tiemersma, E., Willems, R. J. L., Huijsdens, X. W., De Neeling, A. J. & Etienne, J. ( 2005; ). Emergence of virulent methicillin-resistant Staphylococcus aureus strains carrying Panton-Valentine leucocidin genes in The Netherlands. J Clin Microbiol 43, 3341–3345.[CrossRef]
    [Google Scholar]
  48. Willard, D., Monteil, H., Piemont, Y., Assi, R., Messer, J., Lavillaureix, J., Minck, R. & Gauder, R. ( 1982; ). L'exfoliatine dans les staphylococcies néonatales. Nouv Presse Med 11, 3769–3771.
    [Google Scholar]
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