1887

Abstract

Previous experimental investigations of the crystalline biofilms that colonize and block urinary catheters have focussed on their formation by pure cultures of In the urine of patients undergoing long-term catheterization, is commonly found in mixed communities with other urinary tract pathogens. Little is known about the effect that the other species have on the rate at which encrusts catheters. In the present study, a set of data on the nature of the bacterial communities on 106 catheter biofilms has been analysed and it was found that while species such as and were commonly associated with , when , or were present, was rarely or never found. The hypothesis that the absence of from some biofilm communities could be due to its active exclusion by other species has also been examined. Experiments in laboratory models showed that co-infection of with or had no effect on the ability of to encrust and block catheters. Co-infection with or , however, significantly increased the time that catheters took to block ( <0.05). The growth of , , or in the model for 72 h prior to superinfection with significantly delayed catheter blockage. In the case of , for example, the mean time to blockage was extended from 28.7 h to 60.7 h ( ≤0.01). In all cases, however, was able to generate alkaline urine, colonize the biofilms, induce crystal formation and block the catheters. The results suggest that although there is a degree of antagonism between and some of the other urinary tract organisms, the effects are temporary and whatever the pre-existing urinary microbiota, infection with is thus likely to lead to catheter encrustation and blockage.

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2007-11-01
2024-12-06
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References

  1. Anderson J. D., Eftekhar F., Aird M. Y., Hammond J. 1979; The role of bacterial growth rates in the epidemiology and pathogenesis of urinary infections in women. J Clin Microbiol 10:766–771
    [Google Scholar]
  2. Clayton C. L., Chawla J. C., Stickler D. J. 1982; Some observations on urinary tract infections in patients undergoing long-term bladder catheterization. J Hosp Infect 3:39–47 [CrossRef]
    [Google Scholar]
  3. Ganderton L., Chawla J., Winters C., Wimpenny J., Stickler D. 1992; Scanning electron microscopy of bacterial biofilms on indwelling bladder catheters. Eur J Clin Microbiol Infect Dis 11:789–796 [CrossRef]
    [Google Scholar]
  4. Griffith D. P., Musher D. M., Itin C. 1976; Urease. The primary cause of infection induced urinary stones. Invest Urol 13:346–350
    [Google Scholar]
  5. Kohler-Ockmore J., Feneley R. C. L. 1996; Long-term catheterization of the bladder: prevalence and morbidity. Br J Urol 77:347–351 [CrossRef]
    [Google Scholar]
  6. Kunin C. M. 1989; Blockage of urinary catheters: role of microorganisms and constituents of the urine on formation of encrustations. J Clin Epidemiol 42:835–842 [CrossRef]
    [Google Scholar]
  7. Kunin C. M. 1997; Care of the urinary catheter. In Urinary Tract Infections: Detection, Prevention and Management , 5th edn. pp 226–279 Baltimore: Williams & Wilkins;
    [Google Scholar]
  8. Matsukawa M., Kunishima Y., Takahashi S., Takeyama K., Tsukamoto T. 2005; Bacterial colonization on intraluminal surface of urethral catheter. Urology 65:440–444 [CrossRef]
    [Google Scholar]
  9. Mobley H. L. T., Warren J. W. 1987; Urease-positive bacteriuria and obstruction of long-term urinary catheters. J Clin Microbiol 25:2216–2217
    [Google Scholar]
  10. Morris N. S., Stickler D. J. 1998; Encrustation of indwelling urethral catheters by Proteus mirabilis biofilms growing in human urine. J Hosp Infect 39:227–234 [CrossRef]
    [Google Scholar]
  11. Morris N. S., Stickler D. J., Winters C. 1997; Which indwelling urethral catheters resist encrustation by Proteus mirabilis biofilms?. Br J Urol 80:58–63 [CrossRef]
    [Google Scholar]
  12. Morris N. S., Stickler D. J., McLean R. J. C. 1999; The development of bacterial biofilms on indwelling urethral catheters. World J Urol 17:345–350 [CrossRef]
    [Google Scholar]
  13. Ohkawa M., Sugata T., Sawaki M., Nakashima T., Fuse H., Hisazumi H. 1990; Bacterial and crystal adherence to the surfaces of indwelling urethral catheters. J Urol 143:717–721
    [Google Scholar]
  14. Stickler D. J., Morris N. S., Winters C. 1999; Simple physical model to study formation and physiology of biofilms on urethral catheters. Methods Enzymol 310:494–501
    [Google Scholar]
  15. Warren J. W., Damron D., Tenney J. H., Hoopes J. M., Deforge B., Muncie H. L. 1987; Fever, bacteraemia and death as complications of bacteriuria in women with long-term urethral catheters. J Infect Dis 155:1151–1158 [CrossRef]
    [Google Scholar]
  16. Winters C., Stickler D. J., Howe N. S., Williams T. G., Wilkinson N., Buckley C. J. 1995; Some observations on the structure of encrusting biofilms of Proteus mirabilis on urethral catheters. Cells Mater 5:245–253
    [Google Scholar]
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