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Volume 56, Issue 9

Artemisinin triggers induction of cell-cycle arrest and apoptosis in promastigotes Free

Abstract

A major impediment to effective anti-leishmanial chemotherapy is the emergence of drug resistance, especially to sodium antimony gluconate, the first-line treatment for leishmaniasis. Artemisinin, a sesquiterpene lactone isolated from , is an established anti-malarial compound that showed anti-leishmanial activity in both promastigotes and amastigotes, with IC values of 160 and 22 μM, respectively, and, importantly, was accompanied by a high safety index (>22-fold). The leishmanicidal activity of artemisinin was mediated via apoptosis as evidenced by externalization of phosphatidylserine, loss of mitochondrial membrane potential, labelling of DNA fragments by terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labelling (TUNEL) and cell-cycle arrest at the sub-G/G phase. Taken together, these data indicate that artemisinin has promising anti-leishmanial activity that is mediated by programmed cell death and, accordingly, merits consideration and further investigation as a therapeutic option for the treatment of leishmaniasis.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): MTS, 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethaloxyphenyl)-2-(4-sulfonyl)-2H-tetrazolium inner salt , PI, propidium iodide , TdT, terminal deoxynucleotidyl transferase and TUNEL, TdT-mediated dUTP nick end labelling
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2007-09-01
2024-04-19
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Artemisinin triggers induction of cell-cycle arrest and apoptosis in Leishmania donovani promastigotes
J. Med. Microbiol. 56, 1213 (2007); https://doi.org/10.1099/jmm.0.47364-0
/content/journal/jmm/10.1099/jmm.0.47364-0
/content/journal/jmm/10.1099/jmm.0.47364-0
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