1887

Abstract

A multiplex PCR assay has been developed for the identification of the six common taxa associated with human gastroenteritis and/or septicaemia, namely , , subsp. , , and . The assay was developed using a combination of newly designed and published primers. It provided a specific PCR product for each of the five species and the one subspecies, and each of the PCR products was sufficiently distinguished by a difference in size by agarose gel electrophoresis. On evaluation of efficacy with 142 strains, the assay correctly identified all strains as 1 of the 6 taxa. This multiplex PCR assay is a rapid, simple and practical tool for identification of the six taxa commonly associated with gastroenteritis and/or septicaemia in humans, and offers an effective alternative to conventional biochemical-based assays.

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2007-11-01
2019-10-18
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References

  1. Broczyk, A., Thompson, S., Smith, D. & Lior, H. ( 1987; ). Water-borne outbreak of Campylobacter laridis-associated gastroenteritis. Lancet 1, 164–165.
    [Google Scholar]
  2. Devane, M. L., Nicol, C., Ball, A., Klena, J. D., Scholes, P., Hudson, J. A., Baker, M. G., Gilpin, B. J., Garrett, N. & Savill, M. G. ( 2005; ). The occurrence of Campylobacter subtypes in environmental reservoirs and potential transmission routes. J Appl Microbiol 98, 980–990.[CrossRef]
    [Google Scholar]
  3. Engberg, J., On, S. L., Harrington, C. S. & Gerner-Smidt, P. ( 2000; ). Prevalence of Campylobacter, Arcobacter, Helicobacter, and Sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. J Clin Microbiol 38, 286–291.
    [Google Scholar]
  4. Goossens, H., Giesendorf, B. A., Vandamme, P., Vlaes, L., Van den Borre, C., Koeken, A., Quint, W. G., Blomme, W., Hanicq, P. & Koster, D. S. ( 1995; ). Investigation of an outbreak of Campylobacter upsaliensis in day care centers in Brussels: analysis of relationships among isolates by phenotypic and genotypic typing methods. J Infect Dis 172, 1298–1305.[CrossRef]
    [Google Scholar]
  5. Harrington, C. S. & On, S. L. W. ( 1999; ). Extensive 16S rRNA gene sequence diversity in Campylobacter hyointestinalis strains: taxonomic and applied implications. Int J Syst Bacteriol 49, 1171–1175.[CrossRef]
    [Google Scholar]
  6. Hum, S., Quinn, K., Brunner, J. & On, S. L. W. ( 1997; ). Evaluation of a PCR assay for identification and differentiation of Campylobacter fetus subspecies. Aust Vet J 75, 827–831.[CrossRef]
    [Google Scholar]
  7. Inglis, G. D. & Kalischuk, L. D. ( 2003a; ). Use of PCR for direct detection of Campylobacter species in bovine feces. Appl Environ Microbiol 69, 3435–3447.[CrossRef]
    [Google Scholar]
  8. Inglis, G. D., Kalischuk, L. D. & Busz, H. W. ( 2003b; ). A survey of Campylobacter species shed in faeces of beef cattle using polymerase chain reaction. Can J Microbiol 49, 655–661.[CrossRef]
    [Google Scholar]
  9. Klena, J. D., Parker, C. T., Knibb, K., Ibbitt, J. C., Devane, P. M., Horn, S. T., Miller, W. G. & Konkel, M. E. ( 2004; ). Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA. J Clin Microbiol 42, 5549–5557.[CrossRef]
    [Google Scholar]
  10. Lastovica, A. J. & Skirrow, M. B. ( 2000; ): Clinical significance of Campylobacter and related species other than Campylobacter jejuni and C. coli. In Campylobacter, 2nd edn, pp. 89–120. Edited by I. Nachamkin & M. J. Blaser. Washington, DC: American Society for Microbiology.
  11. Lawson, A. J., Logan, J. M., O'Neill, G. L., Desai, M. & Stanley, J. ( 1999; ). Large-scale survey of Campylobacter species in human gastroenteritis by PCR and PCR-enzyme-linked immunosorbent assay. J Clin Microbiol 37, 3860–3864.
    [Google Scholar]
  12. Linton, D., Owen, R. J. & Stanley, J. ( 1996; ). Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals. Res Microbiol 147, 707–718.[CrossRef]
    [Google Scholar]
  13. Linton, D., Lawson, A. J., Owen, R. J. & Stanley, J. ( 1997; ). PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples. J Clin Microbiol 35, 2568–2572.
    [Google Scholar]
  14. Logan, J. M., Edwards, K. J., Saunders, N. A. & Stanley, J. ( 2001; ). Rapid identification of Campylobacter spp. by melting peak analysis of biprobes in real-time PCR. J Clin Microbiol 39, 2227–2232.[CrossRef]
    [Google Scholar]
  15. Macuch, P. J. & Tanner, A. C. ( 2000; ). Campylobacter species in health, gingivitis, and periodontitis. J Dent Res 79, 785–792.[CrossRef]
    [Google Scholar]
  16. Misawa, N., Kawashima, K., Kawamoto, H. & Kondo, F. ( 2002; ). Development of a combined filtration-enrichment culture followed by a one-step duplex PCR technique for the rapid detection of Campylobacter jejuni and C. coli in human faecal samples. J Med Microbiol 51, 86–89.
    [Google Scholar]
  17. Moore, J. E., Corcoran, D., Dooley, J. S., Fanning, S., Lucey, B., Matsuda, M., McDowell, D. A., Mégraud, F., Millar, B. C., O'Mahony, R. & other authors ( 2005; ). Campylobacter. Vet Res 36, 351–382.[CrossRef]
    [Google Scholar]
  18. Morooka, T., Umeda, A., Fujita, M., Matano, H., Fujimoto, S., Yukitake, K., Amako, K. & Oda, T. ( 1996; ). Epidemiologic application of pulsed-field gel electrophoresis to an outbreak of Campylobacter fetus meningitis in a neonatal intensive care unit. Scand J Infect Dis 28, 269–270.[CrossRef]
    [Google Scholar]
  19. Müller, W., Hotzel, H. & Schulze, F. ( 2003; ). Identification and differentiation of Campylobacter fetus subspecies using PCR. Dtsch Tierarztl Wochenschr 110, 55–59.
    [Google Scholar]
  20. Nachamkin, I. ( 2003; ). Campylobacter and Arcobacter. In Manual of Clinical Microbiology, vol. 1, 8th edn, pp. 902–914. Edited by P. R. Murray, E. J. Baron J. H. Jorgensen, M. A. Pfaller & R. H. Yolken. Washington, DC: American Society for Microbiology.
  21. On, S. L. W. ( 2005; ): Taxonomy, phylogeny, and methods for the identification of Campylobacter species. In Campylobacter Molecular and Cellular Biology, pp. 13–42. Edited by J. M. Ketley & M. E. Konkel. Norfolk: Horizon Bioscience.
  22. On, S. L. W. & Holmes, B. ( 1991; ). Effect of inoculum size on the phenotypic characterization Campylobacter species. J Clin Microbiol 29, 923–926.
    [Google Scholar]
  23. On, S. L. W. & Jordan, P. J. ( 2003; ). Evaluation of 11 PCR assays for species-level identification of Campylobacter jejuni and Campylobacter coli. J Clin Microbiol 41, 330–336.[CrossRef]
    [Google Scholar]
  24. On, S. L. W. & Vandamme, P. ( 1997; ). Identification and epidemiological typing of Campylobacter hyointestinalis subspecies by phenotypic and genomic methods and description of novel subgroups. Syst Appl Microbiol 20, 238–247.[CrossRef]
    [Google Scholar]
  25. Parkhill, J., Wren, B. W., Mungall, K., Ketley, J. M., Churcher, C., Basham, D., Chillingworth, T., Davies, R. M., Feltwell, T. & other authors ( 2000; ). The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature 403, 665–668.[CrossRef]
    [Google Scholar]
  26. Salama, S. M., Tabor, H., Richter, M. & Taylor, D. E. ( 1992; ). Pulsed-field gel electrophoresis for epidemiologic studies of Campylobacter hyointestinalis isolates. J Clin Microbiol 30, 1982–1984.
    [Google Scholar]
  27. Savill, M. G., Hudson, J. A., Ball, A., Klena, J. D., Scholes, P., Whyte, R. J., McCormick, R. E. & Jankovic, D. ( 2001; ). Enumeration of Campylobacter in New Zealand recreational and drinking waters. J Appl Microbiol 91, 38–46.[CrossRef]
    [Google Scholar]
  28. Van Etterijck, R., Breynaert, J., Revets, H., Devreker, T., Vandenplas, Y., Vandamme, P. & Lauwers, S. ( 1996; ). Isolation of Campylobacter concisus from feces of children with and without diarrhea. J Clin Microbiol 34, 2304–2306.
    [Google Scholar]
  29. Wang, R. F., Slavic, M. F. & Cao, W. W. ( 1992; ). A rapid PCR method for direct detection of low numbers of Campylobacter jejuni. J Rapid Methods Autom Microbiol 1, 101–108.[CrossRef]
    [Google Scholar]
  30. Wang, G., Clark, C. G., Taylor, T. M., Pucknell, C., Barton, C., Price, L., Woodward, D. L. & Rodgers, F. G. ( 2002; ). Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus. J Clin Microbiol 40, 4744–4747.[CrossRef]
    [Google Scholar]
  31. Werno, A. M., Klena, J. D., Shaw, G. M. & Murdoch, D. R. ( 2002; ). Fatal case of Campylobacter lari prosthetic joint infection and bacteremia in an immunocompetent patient. J Clin Microbiol 40, 1053–1055.[CrossRef]
    [Google Scholar]
  32. Willoughby, K., Nettleton, P. F., Quirie, M., Maley, M. A., Foster, G., Toszeghy, M. & Newell, D. G. ( 2005; ). A multiplex polymerase chain reaction to detect and differentiate Campylobacter fetus subspecies fetus and Campylobacter fetus -species venerealis: use on UK isolates of C. fetus and other Campylobacter spp. J Appl Microbiol 99, 758–766.[CrossRef]
    [Google Scholar]
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