1887

Abstract

A multiplex PCR assay has been developed for the identification of the six common taxa associated with human gastroenteritis and/or septicaemia, namely , , subsp. , , and . The assay was developed using a combination of newly designed and published primers. It provided a specific PCR product for each of the five species and the one subspecies, and each of the PCR products was sufficiently distinguished by a difference in size by agarose gel electrophoresis. On evaluation of efficacy with 142 strains, the assay correctly identified all strains as 1 of the 6 taxa. This multiplex PCR assay is a rapid, simple and practical tool for identification of the six taxa commonly associated with gastroenteritis and/or septicaemia in humans, and offers an effective alternative to conventional biochemical-based assays.

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2007-11-01
2020-04-05
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