1887

Abstract

Capsular serotype surveillance of clinical isolates of is essential for evaluation of the potential impact of introducing multivalent capsular serotype-based vaccines in Latin America. Here, a previously described sequential multiplex PCR method was revised for optimal targeting of prevalent serotypes in Latin America. The revised protocol successfully serotyped 139/147 pneumococci (94.6 %) from Brazilian children, demonstrating a labour-efficient, accurate method requiring only conventional PCR capability.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47347-0
2007-09-01
2021-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/9/1185.html?itemId=/content/journal/jmm/10.1099/jmm.0.47347-0&mimeType=html&fmt=ahah

References

  1. Arbique J. C., Poyart C., Trieu-Cuot P., Quesne G., Carvalho M. G. S., Steigerwalt A. G., Morey R. E., Jackson D., Davidson R. J., Facklam R. R. 2004; Accuracy of phenotypic and genotypic testing for identification of Streptococcus pneumoniae and description of Streptococcus pseudopneumoniae sp. nov. J Clin Microbiol 42:4686–4696 [CrossRef]
    [Google Scholar]
  2. Brito D. A., Ramirez M., de Lencastre H. 2003; Serotyping Streptococcus pneumoniae by multiplex PCR. J Clin Microbiol 41:2378–2384 [CrossRef]
    [Google Scholar]
  3. Di Fabio J. L., Castañeda E., Agudelo C. I., De la Hoz F., Hortal M., Camou T., Echaniz-Avilés G., Noemi M., Barajas C. other authors 2001; Evolution of Streptococcus pneumoniae serotypes and penicillin susceptibility in Latin America, Sireva-Vigía Group, 1993 to 1999. The PAHO Sireva-Vigía Study Group. Pediatr Infect Dis J 20:959–967 [CrossRef]
    [Google Scholar]
  4. Garcia S., Levine O. S., Cherian T., Gabastou J. M., Andrus J. Working Group Members 2006; Pneumococcal disease and vaccination in the Americas: an agenda for accelerated vaccine introduction. Rev Panam Salud Publica 19:340–348 [CrossRef]
    [Google Scholar]
  5. Ghaffar F., Barton T., Lozano J., Muniz L. S., Hicks P., Gan V., Ahmad N., McCraken G. H. Jr 2004; Effect of the 7-valent pneumococcal conjugate vaccine on nasopharyngeal colonization by Streptococcus pneumoniae in the first 2 years of life. Clin Infect Dis 39:930–938 [CrossRef]
    [Google Scholar]
  6. Hausdorff W. P., Bryant J., Paradiso P. R., Siber G. R. 2000; Which pneumococcal serogroups cause the most invasive disease: implications for conjugate vaccine formulation and use, part I. Clin Infect Dis 30:100–121 [CrossRef]
    [Google Scholar]
  7. Kyaw M. H., Lynfield R., Schaffner W., Craig A. S., Hadler J., Reingold A., Thomas A. R., Harrison L. H., Bennett N. M. other authors 2006; Effect of introduction of the pneumococcal conjugate vaccine on drug-resistant Streptococcus pneumoniae . N Engl J Med 354:1455–1463 [CrossRef]
    [Google Scholar]
  8. Laval C. B., de Andrade A. L., Pimenta F. C., de Andrade J. G., de Oliveira R. M., Silva S. A., de Lima E. C., Fabio J. L., Casagrande S. T., Brandileone M. C. 2006; Serotypes of carriage and invasive isolates of Streptococcus pneumoniae in Brazilian children in the era of pneumococcal vaccines. Clin Microbiol Infect 12:50–55 [CrossRef]
    [Google Scholar]
  9. Morais L., Carvalho Mda. G., Roca A., Flannery B., Mandomando I., Soriano-Gabarró M., Sigauque B., Alonso P., Beall B. 2007; Sequential multiplex PCR for identifying pneumococcal capsular serotypes from south-Saharan African clinical isolates. J Med Microbiol 56:1181–1184 [CrossRef]
    [Google Scholar]
  10. Pai R., Gertz R. E., Beall B. 2006; Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates. J Clin Microbiol 44:124–131 [CrossRef]
    [Google Scholar]
  11. Park I. H., Pritchard D. G., Cartee R., Brandao A., Brandileone M. C., Nahm M. H. 2007; Discovery of a new capsular serotype (6C) within serogroup 6 of Streptococcus pneumoniae . J Clin Microbiol 45:1225–1233 [CrossRef]
    [Google Scholar]
  12. Ray G. T., Whitney C. G., Fireman B. H., Ciuryla V., Black S. B. 2006; Cost-effectiveness of pneumococcal conjugate vaccine: evidence from the first 5 years of use in the United States incorporating herd effects. Pediatr Infect Dis J 25:494–501 [CrossRef]
    [Google Scholar]
  13. Robinson K. A., Baughman W., Rothrock G., Barrett N. L., Pass M., Lexau C., Lamaske B., Stefonek K., Barnes B. other authors 2001; Epidemiology of invasive Streptococcus pneumoniae infection in the United States 1995–1998. Active Bacterial Core Surveillance (ABCs)/Emerging Infections Program Network. JAMA 285:1729–1735 [CrossRef]
    [Google Scholar]
  14. Whitney C. G., Farley M. M., Hadler J., Harrison L. H., Bennett N. M., Lynfield R., Reingold A., Cieslak P. R., Pilishvili T. other authors 2003; Decline in invasive pneumococcal disease after the introduction of protein-polysaccharide conjugate vaccine. Active Bacterial Core Surveillance (ABCs)/Emerging Infections Program Network. N Engl J Med 348:1737–1746 [CrossRef]
    [Google Scholar]
  15. Whitney C. G., Pilishivili T., Farley M. M., Schaffner W., Craig A. S., Lynfield R., Nyquist A. C., Gershman K. A., Vazquez M. other authors 2006; Effectiveness of seven-valent pneumococcal conjugate vaccine against invasive pneumococcal disease: a matched case-control study. Lancet 368:1495–1502 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47347-0
Loading
/content/journal/jmm/10.1099/jmm.0.47347-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error