1887

Abstract

is one of the leading causes of food-borne gastroenteritis. Because of the high prevalence of in poultry, poultry meat is considered a major source of infections for humans. However, it is not known whether all poultry-associated strains are capable of causing disease in humans. Four different virulence properties of strains were compared between 20 poultry isolates and 24 human isolates. Strains were chosen based on their PFGE pattern to represent a heterogeneous population. The isolates were compared for their ability to invade and induce interleukin-8 (IL-8) production in T84 cells, their production of functional cytolethal distending toxin (CDT) using HEp-2 cells, and their sodium deoxycholate resistance. All four virulence factors were present among strains of human and poultry origin, with strong differences observed among strains. For invasion and IL-8 induction, no difference was observed between the two populations. However, on average, human isolates arrested more HEp-2 cells in their cell cycle than did the poultry isolates (=0.041), suggesting higher CDT production by the former. The ability to survive 16 000 μg sodium deoxycholate ml was significantly more pronounced (=0.006) among human isolates than poultry isolates, although all strains possessed the operon. These data suggest that all four virulence properties are widespread among isolates, but that a higher degree of bile-salt resistance and more pronounced CDT production are associated with strains causing enteritis in humans.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47342-0
2007-10-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/10/1284.html?itemId=/content/journal/jmm/10.1099/jmm.0.47342-0&mimeType=html&fmt=ahah

References

  1. Abuoun M., Manning G., Cawthraw S. A., Ridley A., Ahmed I. H., Wassenaar T. M., Newell D. G. 2005; Cytolethal distending toxin (CDT)-negative Campylobacter jejuni strains and anti-CDT neutralizing antibodies are induced during human infection but not during colonization in chickens. Infect Immun 73:3053–3062 [CrossRef]
    [Google Scholar]
  2. Bacon D. J., Alm R. A., Burr D. H., Hu L., Kopecko D. J., Ewing C. P., Trust T. J., Guerry P. 2000; Involvement of a plasmid in virulence of Campylobacter jejuni 81-176. Infect Immun 68:4384–4390 [CrossRef]
    [Google Scholar]
  3. Bang D. D., Scheutz F., Ahrens P., Pedersen K., Blom J., Madsen M. 2001; Prevalence of cytolethal distending toxin ( cdt ) genes and CDT production in Campylobacter spp. isolated from Danish broilers. J Med Microbiol 50:1087–1094
    [Google Scholar]
  4. Bang D. D., Nielsen E. M., Scheutz F., Pedersen K., Handberg K., Madsen M. 2003; PCR detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from Danish pigs and cattle and cytolethal distending toxin production of the isolates. J Appl Microbiol 94:1003–1014 [CrossRef]
    [Google Scholar]
  5. Chien C. C., Taylor N. S., Ge Z., Schauer D. B., Young V. B., Fox J. G. 2000; Identification of cdtB homologues and cytolethal distending toxin activity in enterohepatic Helicobacter spp. J Med Microbiol 49:525–534
    [Google Scholar]
  6. Corry J. E., Atabay H. I. 2001; Poultry as a source of Campylobacter and related organisms. Symp Ser Soc Appl Microbiol 30:96S–114S
    [Google Scholar]
  7. Eyigor A., Dawson K. A., Langlois B. E., Pickett C. L. 1999; Detection of cytolethal distending toxin activity and cdt genes in Campylobacter spp. isolated from chicken carcasses. Appl Environ Microbiol 65:1501–1505
    [Google Scholar]
  8. Fauchere J. L., Rosenau A., Veron M., Moyen E. N., Richard S., Pfister A. 1986; Association with HeLa cells of Campylobacter jejuni and Campylobacter coli isolated from human feces. Infect Immun 54:283–287
    [Google Scholar]
  9. Gilbert C., Slavik M. 2004; Determination of toxicity of Campylobacter jejuni isolated from humans and from poultry carcasses acquired at various stages of production. J Appl Microbiol 97:347–353 [CrossRef]
    [Google Scholar]
  10. Hänninen M. L., Hakkinen M., Rautelin H. 1999; Stability of related human and chicken Campylobacter jejuni genotypes after passage through chick intestine studied by pulsed-field gel electrophoresis. Appl Environ Microbiol 65:2272–2275
    [Google Scholar]
  11. Hänninen M. L., Perko-Makela P., Pitkala A., Rautelin H. 2000; A three-year study of Campylobacter jejuni genotypes in humans with domestically acquired infections and in chicken samples from the Helsinki area. J Clin Microbiol 38:1998–2000
    [Google Scholar]
  12. Herman L., Heyndrickx M., Grijspeerdt K., Vandekerchove D., Rollier I., De Zutter L. 2003; Routes for Campylobacter contamination of poultry meat: epidemiological study from hatchery to slaughterhouse. Epidemiol Infect 131:1169–1180 [CrossRef]
    [Google Scholar]
  13. Hickey T. E., Baqar S., Bourgeois A. L., Ewing C. P., Guerry P. 1999; Campylobacter jejuni -stimulated secretion of interleukin-8 by INT407 cells. Infect Immun 67:88–93
    [Google Scholar]
  14. Hickey T. E., McVeigh A. L., Scott D. A., Michielutti R. E., Bixby A., Carroll S. A., Bourgeois A. L., Guerry P. 2000; Campylobacter jejuni cytolethal distending toxin mediates release of interleukin-8 from intestinal epithelial cells. Infect Immun 68:6535–6541 [CrossRef]
    [Google Scholar]
  15. Konkel M. E., Joens L. A. 1989; Adhesion to and invasion of HEp-2 cells by Campylobacter spp. Infect Immun 57:2984–2990
    [Google Scholar]
  16. Konkel M. E., Monteville M. R., Rivera-Amill V., Joens L. A. 2001; The pathogenesis of Campylobacter jejuni -mediated enteritis. Curr Issues Intest Microbiol 2:55–71
    [Google Scholar]
  17. Lin J., Michel L. O., Zhang Q. 2002; CmeABC functions as a multidrug efflux system in Campylobacter jejuni . Antimicrob Agents Chemother 46:2124–2131 [CrossRef]
    [Google Scholar]
  18. Lin J., Sahin O., Michel L. O., Zhang Q. 2003; Critical role of multidrug efflux pump CmeABC in bile resistance and in vivo colonization of Campylobacter jejuni . Infect Immun 71:4250–4259 [CrossRef]
    [Google Scholar]
  19. Lindblom G. B., Kaijser B. 1995; In vitro studies of Campylobacter jejuni / coli strains from hens and humans regarding adherence, invasiveness, and toxigenicity. Avian Dis 39:718–722 [CrossRef]
    [Google Scholar]
  20. Pearson A. D., Greenwood M. H., Donaldson J., Healing T. D., Jones D. M., Shahamat M., Feltham R. K., Colwell R. R. 2000; Continuous source outbreak of campylobacteriosis traced to chicken. J Food Prot 63:309–314
    [Google Scholar]
  21. Pickett C. L., Pesci E. C., Cottle D. L., Russell G., Erdem A. N., Zeytin H. 1996; Prevalence of cytolethal distending toxin production in Campylobacter jejuni and relatedness of Campylobacter sp. cdtB gene. Infect Immun 64:2070–2078
    [Google Scholar]
  22. Prasad K. N., Dhole T. N., Ayyagari A. 1996; Adherence, invasion and cytotoxin assay of Campylobacter jejuni in HeLa and HEp-2 cells. J Diarrhoeal Dis Res 14:255–259
    [Google Scholar]
  23. Purdy D., Buswell C. M., Hodgson A. E., McAlpine K., Henderson I., Leach S. A. 2000; Characterisation of cytolethal distending toxin (CDT) mutants of Campylobacter jejuni . J Med Microbiol 49:473–479
    [Google Scholar]
  24. Rozynek E., Dzierzanowska-Fangrat K., Jozwiak P., Popowski J., Korsak D., Dzierzanowska D. 2005; Prevalence of potential virulence markers in Polish Campylobacter jejuni and Campylobacter coli isolates obtained from hospitalized children and from chicken carcasses. J Med Microbiol 54:615–619 [CrossRef]
    [Google Scholar]
  25. Russell R. G., O'Donnoghue M., Blake D. C., Zulty J. Jr, DeTolla L. J. 1993; Early colonic damage and invasion of Campylobacter jejuni in experimentally challenged infant Macaca mulatta . J Infect Dis 168:210–215 [CrossRef]
    [Google Scholar]
  26. Tay S. T., Devi S., Puthucheary S., Kautner I. 1996; In vitro demonstration of the invasive ability of campylobacters . Zentralbl Bakteriol 283:306–313 [CrossRef]
    [Google Scholar]
  27. Wingstrand A., Neimann J., Engberg J., Nielsen E. M., Gerner-Smidt P., Wegener H. C., Molbak K. 2006; Fresh chicken as main risk factor for campylobacteriosis, Denmark. Emerg Infect Dis 12:280–285 [CrossRef]
    [Google Scholar]
  28. Young V. B., Knox K. A., Schauer D. B. 2000; Cytolethal distending toxin sequence and activity in the enterohepatic pathogen Helicobacter hepaticus . Infect Immun 68:184–191 [CrossRef]
    [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.47342-0
Loading
/content/journal/jmm/10.1099/jmm.0.47342-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error