1887

Abstract

The major cause of chemotherapy failure in patients with chronic gastritis and peptic ulcers caused by is clarithromycin (CAM) resistance due to a mutation in the 23S rRNA gene. This study describes a non-invasive and accurate method for the detection of mixed CAM-resistant and -susceptible by sequencing of the 23S rRNA gene. Faeces were crushed with beads and the 23S rRNA gene was amplified using a nested PCR on the extracted DNA. Mutation analysis of this gene using this method showed that 20.4 % of patients carried mixed CAM-susceptible (wild type) and -resistant (A2142G or A2143G mutant) . Furthermore, it was found that 66.6 % of patients who had been treated unsuccessfully carried one of these mutations in the 23S rRNA gene (including the mixed type), whilst standard culture detected CAM-resistant isolates in only 22.2 % of patients with unsuccessful treatment. These data suggest that, for successful therapy, the diagnosis method described here would more accurately detect CAM-resistant , including mixed infections.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47302-0
2007-09-01
2019-11-13
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/9/1174.html?itemId=/content/journal/jmm/10.1099/jmm.0.47302-0&mimeType=html&fmt=ahah

References

  1. Akiyama, H., Watanabe, T., Wakabayashi, K., Nakade, S., Yasui, S., Sakata, K., Chiba, R., Spiegelhalter, F., Hino, A. & Maitani, T. ( 2005; ). Quantitative detection system for maize sample containing combined-trait genetically modified maize. Anal Chem 77, 7421–7428.[CrossRef]
    [Google Scholar]
  2. Alarcon, T., Vega, A. E., Domingo, D., Martinez, M. J. & Lopez-Brea, M. ( 2003; ). Clarithromycin resistance among Helicobacter pylori strains isolated from children: prevalence and study of mechanism of resistance by PCR-restriction fragment length polymorphism analysis. J Clin Microbiol 41, 486–499.[CrossRef]
    [Google Scholar]
  3. Asaka, M., Satoh, K., Sugano, K., Sugiyama, T., Takahashi, S., Fukuda, Y., Ota, H., Murakami, K., Kimura, K. & Shimoyama, T. ( 2001; ). Guidelines in the management of Helicobacter pylori infection in Japan. Helicobacter 6, 177–186.[CrossRef]
    [Google Scholar]
  4. Booka, M., Okuda, M., Shin, K., Miyashiro, E., Hayashi, H., Yamauchi, K., Tamura, Y. & Yoshikawa, N. ( 2005; ). Polymerase chain reaction-restriction fragment length polymorphism analysis of clarithromycin-resistant Helicobacter pylori infection in children using stool sample. Helicobacter 10, 205–213.[CrossRef]
    [Google Scholar]
  5. CLSI ( 2002; ). Performance Standards for Antimicrobial Susceptibility Testing: Twelfth Informational Supplement. Wayne, PA: Clinical and Laboratory Standards Institute.
  6. Fontana, C., Favaro, M., Minelli, S., Criscuolo, A. A., Pietroiusti, A., Galante, A. & Favalli, C. ( 2002; ). New site of modification of 23S rRNA associated with clarithromycin resistance of Helicobacter pylori clinical isolates. Antimicrob Agents Chemother 46, 3765–3769.[CrossRef]
    [Google Scholar]
  7. Fontana, C., Favaro, M., Pietroiusti, A., Pistoia, E. S., Galante, A. & Favalli, C. ( 2003; ). Detection of clarithromycin-resistant Helicobacter pylori in stool samples. J Clin Microbiol 41, 3636–3640.[CrossRef]
    [Google Scholar]
  8. Furuta, T., Shirai, N., Takashima, M., Xiao, F., Hanai, H., Sugimura, H., Ohashi, K., Ishizaki, T. & Kaneko, E. ( 2001; ). Effect of genotypic differences in CYP2C19 on cure rates for Helicobacter pylori infection by triple therapy with a proton pump inhibitor, amoxicillin, and clarithromycin. Clin Pharmacol Ther 69, 158–168.[CrossRef]
    [Google Scholar]
  9. Goodwin, C. S., Blincow, E. D., Warren, J. R., Waters, T. E., Sanderson, C. R. & Easton, L. ( 1985; ). Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa. J Clin Pathol 38, 1127–1131.[CrossRef]
    [Google Scholar]
  10. Graham, D. Y., Klein, P. D., Evans, D. J., Jr, Evans, D. G., Alpert, L. C., Opekun, A. R. & Boutton, T. W. ( 1987; ). Campylobacter pylori detected noninvasively by the 13C-urea breath test. Lancet i, 1174–1177.
    [Google Scholar]
  11. Hoshiya, S., Watanabe, K., Tokunaga, K., Tanaka, A., Ninomiya, H., Shingaki, M., Itoh, T., Saito, S., Ishida, H. & Takahashi, S. ( 2000; ). Relationship between eradication therapy and clarithromycin-resistant Helicobacter pylori in Japan. J Gastroenterol 35, 10–14.
    [Google Scholar]
  12. Ito, M., Tanaka, S., Kim, S., Tahara, K., Kawamura, Y., Sumii, M., Takehara, Y., Hayashi, K., Okamoto, E. & other authors ( 2005; ). A combination of the Helicobacter pylori stool antigen test and urea breath test is useful for clinical evaluation of eradication therapy: a multicenter study. J Gastroenterol Hepatol 20, 1241–1245.[CrossRef]
    [Google Scholar]
  13. Kato, M., Yamaoka, Y., Kim, J. J., Reddy, R., Asaka, M., Kashima, K., Osato, M. S., El-Zaatari, F. A., Graham, D. Y. & Kwon, D. H. ( 2000; ). Regional differences in metronidazole resistance and increasing clarithromycin resistance among Helicobacter pylori isolates from Japan. Antimicrob Agents Chemother 44, 2214–2216.[CrossRef]
    [Google Scholar]
  14. Khan, R., Nahar, S., Sultana, J., Ahmad, M. M. & Rahman, M. ( 2004; ). T2182C mutation in 23S rRNA is associated with clarithromycin resistance in Helicobacter pylori isolates obtained in Bangladesh. Antimicrob Agents Chemother 48, 3567–3569.[CrossRef]
    [Google Scholar]
  15. Kim, J. M., Kim, J. S., Kim, N., Kim, S. G., Jung, H. C. & Song, I. S. ( 2006; ). Comparison of primary and secondary antimicrobial minimum inhibitory concentrations for Helicobacter pylori isolated from Korean patients. Int J Antimicrob Agents 28, 6–13.[CrossRef]
    [Google Scholar]
  16. Kuipers, E. J. ( 1997; ). Helicobacter pylori and the risk and management of associated diseases: gastritis, ulcer disease, atrophic gastritis and gastric cancer. Aliment Pharmacol Ther 11 (Suppl. 1), 71–88.[CrossRef]
    [Google Scholar]
  17. Kusters, J. G., Gerrits, M. M., Van Strijp, J. A. & Vandenbroucke-Grauls, C. M. ( 1997; ). Coccoid forms of Helicobacter pylori are the morphologic manifestation of cell death. Infect Immun 65, 3672–3679.
    [Google Scholar]
  18. Kusters, J. G., van Vliet, A. H. & Kuipers, E. J. ( 2006; ). Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev 19, 449–490.[CrossRef]
    [Google Scholar]
  19. Leodolter, A., Vaira, D., Bazzoli, F., Schutze, K., Hirschl, A., Megraud, F. & Malfertheiner, P. ( 2003; ). European multicentre validation trial of two new non-invasive tests for the detection of Helicobacter pylori antibodies: urine-based ELISA and rapid urine test. Aliment Pharmacol Ther 18, 927–931.[CrossRef]
    [Google Scholar]
  20. Leong, R. W. & Sung, J. J. ( 2002; ). Helicobacter species and hepatobiliary diseases. Aliment Pharmacol Ther 16, 1037–1045.[CrossRef]
    [Google Scholar]
  21. Malfertheiner, P., Megraud, F., O'Morain, C., Hungin, A. P., Jones, R., Axon, A., Graham, D. Y. & Tytgat, G. ( 2002; ). Current concepts in the management of Helicobacter pylori infection – the Maastricht 2-2000 Consensus Report. Aliment Pharmacol Ther 16, 167–180.
    [Google Scholar]
  22. Matsuki, T., Watanabe, K., Fujimoto, J., Kado, Y., Takada, T., Matsumoto, K. & Tanaka, R. ( 2004; ). Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria. Appl Environ Microbiol 70, 167–173.[CrossRef]
    [Google Scholar]
  23. McOrist, A. L., Jackson, M. & Bird, A. R. ( 2002; ). A comparison of five methods for extraction of bacterial DNA from human faecal samples. J Microbiol Methods 50, 131–139.[CrossRef]
    [Google Scholar]
  24. Monteiro, L., Gras, N., Vidal, R., Cabrita, J. & Megraud, F. ( 2001; ). Detection of Helicobacter pylori DNA in human feces by PCR: DNA stability and removal of inhibitors. J Microbiol Methods 45, 89–94.[CrossRef]
    [Google Scholar]
  25. Morris, J. M., Reasonover, A. L., Bruce, M. G., Bruden, D. L., McMahon, B. J., Sacco, F. D., Berg, D. E. & Parkinson, A. J. ( 2005; ). Evaluation of seaFAST, a rapid fluorescent in situ hybridization test, for detection of Helicobacter pylori and resistance to clarithromycin in paraffin-embedded biopsy sections. J Clin Microbiol 43, 3494–3496.[CrossRef]
    [Google Scholar]
  26. Nurgalieva, Z. Z., Opekun, A. R. & Graham, D. Y. ( 2006; ). Problem of distinguishing false-positive tests from acute or transient Helicobacter pylori infections. Helicobacter 11, 69–74.[CrossRef]
    [Google Scholar]
  27. Prokhorenko, I. A., Malakhov, A. D., Kozlova, A. A., Momynaliev, K., Govorun, V. M. & Korshun, V. A. ( 2006; ). Phenylethynylpyrene-labeled oligonucleotide probes for excimer fluorescence SNP analysis of 23S rRNA gene in clarithromycin-resistant Helicobacter pylori strains. Mutat Res 599, 144–151.[CrossRef]
    [Google Scholar]
  28. Rimbara, E., Noguchi, N., Tanabe, M., Kawai, T., Matsumoto, Y. & Sasatsu, M. ( 2005a; ). Susceptibilities to clarithromycin, amoxycillin and metronidazole of Helicobacter pylori isolates from the antrum and corpus in Tokyo, Japan, 1995–2001. Clin Microbiol Infect 11, 307–311.[CrossRef]
    [Google Scholar]
  29. Rimbara, E., Noguchi, N., Yamaguchi, T., Narui, K., Kawai, T. & Sasatsu, M. ( 2005b; ). Development of a highly sensitive method for detection of clarithromycin-resistant Helicobacter pylori from human feces. Curr Microbiol 51, 1–5.[CrossRef]
    [Google Scholar]
  30. Schabereiter-Gurtner, C., Hirschl, A. M., Dragosics, B., Hufnagl, P., Puz, S., Kovach, Z., Rotter, M. & Makristathis, A. ( 2004; ). Novel real-time PCR assay for detection of Helicobacter pylori infection and simultaneous clarithromycin susceptibility testing of stool and biopsy specimens. J Clin Microbiol 42, 4512–4518.[CrossRef]
    [Google Scholar]
  31. Shuber, A. P., Ascano, J. J., Boynton, K. A., Mitchell, A., Frierson, H. F., Jr, El-Rifai, W. & Powell, S. M. ( 2002; ). Accurate, noninvasive detection of Helicobacter pylori DNA from stool samples: potential usefulness for monitoring treatment. J Clin Microbiol 40, 262–264.[CrossRef]
    [Google Scholar]
  32. Thomas, J. E., Gibson, G. R., Darboe, M. K., Dale, A. & Weaver, L. T. ( 1992; ). Isolation of Helicobacter pylori from human faeces. Lancet 340, 1194–1195.[CrossRef]
    [Google Scholar]
  33. Toracchio, S., Aceto, G. M., Mariani-Costantini, R., Battista, P. & Marzio, L. ( 2004; ). Identification of a novel mutation affecting domain V of the 23S rRNA gene in Helicobacter pylori. Helicobacter 9, 396–399.[CrossRef]
    [Google Scholar]
  34. Vaira, D., Malfertheiner, P., Megraud, F., Axon, A. T., Deltenre, M., Hirschl, A. M., Gasbarrini, G., O'Morain, C., Garcia, J. M. & other authors ( 1999; ). Diagnosis of Helicobacter pylori infection with a new non-invasive antigen-based assay. HpSA European study group. Lancet 354, 30–33.[CrossRef]
    [Google Scholar]
  35. van Doorn, L. J., Glupczynski, Y., Kusters, J. G., Mégraud, F., Midolo, P., Maggi-Solcà, N., Queiroz, D. M., Nouhan, N., Stet, E. & Quint, W. G. ( 2001; ). Accurate prediction of macrolide resistance in Helicobacter pylori by a PCR line probe assay for detection of mutations in the 23S rRNA gene: multicenter validation study. Antimicrob Agents Chemother 45, 1500–1504.[CrossRef]
    [Google Scholar]
  36. Wong, B. C., Wang, W. H., Berg, D. E., Fung, F. M., Wong, K. W., Wong, W. M., Lai, K. C., Cho, C. H., Hui, W. M. & Lam, S. K. ( 2001; ). High prevalence of mixed infections by Helicobacter pylori in Hong Kong: metronidazole sensitivity and overall genotype. Aliment Pharmacol Ther 15, 493–503.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47302-0
Loading
/content/journal/jmm/10.1099/jmm.0.47302-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error