1887

Abstract

Coagulase-negative staphylococci (CoNS) are now recognized as the aetiological agents of an important range of infections in humans. Most developed countries have reported an increase in CoNS infections in hospitalized patients that are resistant to meticillin and other antibiotics. Staphylococcal cassette chromosome (SCC) typing is essential for understanding the molecular epidemiology of meticillin-resistant strains. SCC elements are currently classified into types I to VI based on the characteristics of the and gene complexes and are further classified into subtypes according to their ‘junkyard DNA’ region. We evaluated the distribution of SCC types in CoNS from patients attending the Hospital de Clínicas de Porto Alegre over the period August 2004–December 2005. Among the 129 bloodstream isolates, 36 (27.9 %) harboured SCC type I, 4 (3.0 %) harboured SCC type II, 67 (52 %) harboured SCC type III, 1 (0.8 %) harboured SCC type IV and 4 (3.0 %) harboured SCC types I and III. Seventeen isolates were not typable. Identification of CoNS at the species level indicated that was the most common species, with 87 isolates, followed by (15), (13), (12) and (1). SCC type III was the most prevalent among isolates of (52 %). Among these strains, 30 (23 %) harboured a modified SCC type III which contained an additional region in comparison with regular type III. SCC type III was also highly prevalent (75 %) among isolates. The predominant SCC type found among isolates was type I. However, all four isolates harbouring SCC type II belonged to . Our results indicate that SCC type III was the most prevalent among the CoNS. Isolates with SCC type III were more resistant to non--lactam antimicrobials than isolates harbouring SCC types I, II and IV, although the increase in resistance was statistically significant only for clindamycin (=0.021), rifampicin (=0.010) and levofloxacin (=0.005).

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2007-10-01
2019-09-15
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