1887

Abstract

Shiga toxins (Stx1 and Stx2) are responsible for initiating haemolytic uraemic syndrome, a serious extraintestinal complication caused by enterohaemorrhagic O157 : H7 infection in humans. Shiga toxins are classical AB-type exotoxins, consisting of a globotriaosylceramide (Gb)-binding B subunit pentamer and an enzymic A subunit. It is demonstrated in this study that Stx2 binds to human neutrophils by a non-classical mechanism that is independent of Gb. In contrast, the investigation revealed that Stx2 binds to murine neutrophils by the classical Gb-dependent mechanism. Moreover, whereas the human serum amyloid P (HuSAP) component inhibited Stx2 binding to murine neutrophils, HuSAP increased Stx2 binding to human neutrophils by 84.2 % (≤0.002, Student's -test). These observations may explain why HuSAP protects mice from the lethal effects of Stx2, whereas there is no indication that HuSAP plays a similar protective role in humans infected by O157 : H7.

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2007-11-01
2021-02-24
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