1887

Abstract

The authors have previously shown that the periodontal pathogen (formerly ) Y4 contains an operon for a genotoxin known as the cytolethal distending toxin (Cdt). The locus in strain Y4 is flanked by remnants of heterologous plasmid and integrase sequences. In this study, the DNA sequence immediately downstream from the locus on the Y4 chromosome was examined. The extended sequence contained a region that had all the characteristics of a typical bacterial pathogenicity or genomic island. The genomic island (GIY4-1) was approximately 22 kb long, was flanked by a bacteriophage attachment (att) sequence and contained a full-length integrase/resolvase gene (). A total of 22 complete and partial ORFs represented putative DNA replication/DNA binding/conjugation proteins as well as hypothetical proteins. GIY4-1 was most closely related to putative genomic islands in 35000HP and 86-028NP and to a chromosomal region in 129PT. GIY4-1 was not present in HK1651, which was used as the prototype strain for genomic sequencing of . Several sequences in GIY4-1 were homologous to ORFs found on the . plasmid pVT745. None of the identified ORFs in GIY4-1 appeared to encode potential virulence genes. However, several unique observations supported the possibility that the locus of Y4 was originally contained within the genomic island.

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2007-11-01
2019-10-23
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