1887

Abstract

The aim of this study was to characterize human isolates of species for their capacity to interfere with the growth of different strains of species in the search for a potential probiotic. Growth inhibition of species was screened using an agar-overlay method. Inhibiting strains were selected to assay the effect of a cell-free culture filtrate (LCF) on the growth of isolates of and . A total of 126 human isolates was investigated. Eighteen isolates significantly inhibited the growth of on agar. The LCF of one of these strains showed strong inhibition of both and . This strain was genetically identified as and designated Ess-1. Further tests to evaluate the probiotic potential of this strain indicated that Ess-1 strain is a promising probiotic for use in clinical trials to treat and prevent vulvo-vaginal candidiasis.

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2007-11-01
2019-10-15
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References

  1. Demirezen, S. ( 2002; ). The LactobacilliCandida relationship in cervico-vaginal smears. Cent Eur J Public Health 10, 97–99.
    [Google Scholar]
  2. Juarez Tomas, M. S., Ocana, V. S., Wiese, B. & Nader-Macias, M. E. ( 2003; ). Growth and lactic acid production by vaginal Lactobacillus acidophilus CRL 1259, and inhibition of uropathogenic Escherichia coli. J Med Microbiol 52, 1117–1124.[CrossRef]
    [Google Scholar]
  3. Kaewsrichan, J., Peeyananjarassri, K. & Kongprasertkit, J. ( 2006; ). Selection and identification of anaerobic lactobacilli producing inhibitory compounds against vaginal pathogens. FEMS Immunol Med Microbiol 48, 75–83.[CrossRef]
    [Google Scholar]
  4. Kilic, E., Aslim, B. & Taner, Z. ( 2005; ). Susceptibility to some antifungal drugs of vaginal lactobacilli isolated from healthy women. Drug Metabol Drug Interact 21, 67–74.
    [Google Scholar]
  5. Martens, M. G., Hoffman, P. & El-Zaatari, M. ( 2004; ). Fungal species changes in the female genital tract. J Low Genit Tract Dis 8, 21–24.[CrossRef]
    [Google Scholar]
  6. Okkers, D. J., Dicks, L. M., Silvester, M., Joubert, J. J. & Odendaal, H. J. ( 1999; ). Characterization of pentocin TV35b, a bacteriocin-like peptide isolated from Lactobacillus pentosus with a fungistatic effect on Candida albicans. J Appl Microbiol 87, 726–734.[CrossRef]
    [Google Scholar]
  7. Paulitsch, A., Weger, W., Ginter-Hanselmayer, G., Marth, E. & Buzina, W. ( 2006; ). A 5-year (2000–2004) epidemiological survey of Candida and non-Candida yeast species causing vulvovaginal candidiasis in Graz, Austria. Mycoses 49, 471–475.[CrossRef]
    [Google Scholar]
  8. Reid, G. & Bruce, A. W. ( 2003; ). Urogenital infections in women: can probiotics help?. Postgrad Med J 79, 428–432.[CrossRef]
    [Google Scholar]
  9. Richter, S. S., Galask, R. P., Messer, S. A., Hollis, R. J., Diekema, D. J. & Pfaller, M. A. ( 2005; ). Antifungal susceptibilities of Candida species causing vulvovaginitis and epidemiology of recurrent cases. J Clin Microbiol 43, 2155–2162.[CrossRef]
    [Google Scholar]
  10. Rönnqvist, D., Strom, H., Forsgren-Brusk, U. & Grahn-Hakansson, E. ( 2005; ). Selection and characterization of a Lactobacillus plantarum strain promising as a urogenital probiotic. Microb Ecol Health Dis 17, 75–82.[CrossRef]
    [Google Scholar]
  11. Runeman, B., Rybo, G., Forsgren-Brusk, U., Larko, O., Larsson, P. & Faergemann, J. ( 2004; ). The vulvar skin microenvironment: influence of different panty liners on temperature, pH and microflora. Acta Derm Venereol 84, 277–284.
    [Google Scholar]
  12. Sobel, J. D. & Chaim, W. ( 1996; ). Vaginal microbiology of women with acute recurrent vulvovaginal candidiasis. J Clin Microbiol 34, 2497–2499.
    [Google Scholar]
  13. Sobel, J. D., Chaim, W., Nagappan, V. & Leaman, D. ( 2003; ). Treatment of vaginitis caused by Candida glabrata: use of topical boric acid and flucytosine. Am J Obstet Gynecol 189, 1297–1300.[CrossRef]
    [Google Scholar]
  14. Spinillo, A., Capuzzo, E., Gulminetti, R., Marone, P., Colonna, L. & Piazzi, G. ( 1997; ). Prevalence of and risk factors for fungal vaginitis caused by non-albicans species. Am J Obstet Gynecol 176, 138–141.[CrossRef]
    [Google Scholar]
  15. Stiles, J., Penkar, S., Plockova, M., Chumchalova, J. & Bullerman, L. B. ( 2002; ). Antifungal activity of sodium acetate and Lactobacillus rhamnosus. J Food Prot 65, 1188–1191.
    [Google Scholar]
  16. Strus, M., Brzychczy-Wloch, M., Kucharska, A., Gosiewski, T. & Heczko, P. B. ( 2005a; ). Inhibitory activity of vaginal Lactobacillus bacteria on yeasts causing vulvovaginal candidiasis. Med Dosw Mikrobiol 57, 7–17.
    [Google Scholar]
  17. Strus, M., Kucharska, A., Kukla, G., Brzychczy-Wloch, M., Maresz, K. & Heczko, P. B. ( 2005b; ). The in vitro activity of vaginal Lactobacillus with probiotic properties against Candida. Infect Dis Obstet Gynecol 13, 69–75.[CrossRef]
    [Google Scholar]
  18. Vasquez, A., Jakobsson, T., Ahrne, S., Forsum, U. & Molin, G. ( 2002; ). Vaginal lactobacillus flora of healthy Swedish women. J Clin Microbiol 40, 2746–2749.[CrossRef]
    [Google Scholar]
  19. Voravuthikunchai, S. P., Bilasoi, S. & Supamala, O. ( 2006; ). Antagonistic activity against pathogenic bacteria by human vaginal lactobacilli. Anaerobe 12, 221–226.[CrossRef]
    [Google Scholar]
  20. Wilson, J. ( 2004; ). Managing recurrent bacterial vaginosis. Sex Transm Infect 80, 8–11.[CrossRef]
    [Google Scholar]
  21. Wynne, A. G., McCartney, A. L., Brostoff, J., Hudspith, B. N. & Gibson, G. R. ( 2004; ). An in vitro assessment of the effects of broad-spectrum antibiotics on the human gut microflora and concomitant isolation of a Lactobacillus plantarum with anti-Candida activities. Anaerobe 10, 165–169.[CrossRef]
    [Google Scholar]
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