1887

Abstract

Trypanosomes were observed in a peripheral blood smear from a 45-day-old Thai infant displaying fever, anaemia, cough and anorexia. Human trypanosomiasis is not endemic to Thailand, so parasite identification was undertaken to determine likely sources of the infection. Several morphological parameters of the trypanosomes were similar to those of and statistically different from those of -like parasites from a naturally infected indigenous rat. However, duplicate PCR assays with primers flanking trypanosome rRNA internal transcribed spacer 1 (ITS1) resulted in amplicons of ~623 bp that corresponded to the expected size for -like parasites. The ITS1 sequence from the infant's blood was 98 and 49 % identical to and sequences, respectively. Based on molecular results, it was concluded that the infant was infected with a -like () species.

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2007-08-01
2019-11-17
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References

  1. Desquesnes, M., Ravel, S. & Cuny, G. ( 2002; ). PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats. Kinetoplastid Biol Dis 1, 2 [CrossRef]
    [Google Scholar]
  2. Hoare, C. A. ( 1964; ). Morphologic and taxonomic studies on mammalian trypanosomes. X. Revision of the systematics. J Protozool 11, 200–207.[CrossRef]
    [Google Scholar]
  3. Hoare, C. A. ( 1972; ). The Trypanosomes of Mammals. A Zoological Monograph. Oxford and Edinburgh: Blackwell Scientific Publications.
  4. Johnson, P. D. ( 1933; ). A case of infection by Trypanosoma lewisi in a child. Trans R Soc Trop Med Hyg 26, 467 [CrossRef]
    [Google Scholar]
  5. Joshi, P. P., Shegokar, V. R., Powar, R. M., Herder, S., Katti, R., Salkar, H. R., Dani, V. S., Bhargava, A., Jannin, J. & Truc, P. ( 2005; ). Human trypanosomiasis caused by Trypanosoma evansi in India: the first case report. Am J Trop Med Hyg 73, 491–495.
    [Google Scholar]
  6. Linardi, P. M. & Botelho, J. R. ( 2002; ). Prevalence of Trypanosoma lewisi in Rattus norvegicus from Belo Horizonte, State of Minas Gerais, Brazil. Mem Inst Oswaldo Cruz 97, 411–414.[CrossRef]
    [Google Scholar]
  7. Molyneux, D. H. ( 1969; ). Intracellular stages of Trypanosoma lewisi in fleas and attempts to find such stages in other trypanosome species. Parasitology 59, 737–744.
    [Google Scholar]
  8. Natheewattana, N., Hongsbhanich, L., Khamboonruang, C. & Thitasut, P. ( 1973; ). Preliminary study of a Trypanosoma lewisi-like parasite of rats in Chiang Mai, Thailand. Southeast Asian J Trop Med Public Health 4, 322–323.
    [Google Scholar]
  9. Sarataphan, N., Siriwan, C., Punyahotra, R., Mekamol, C., Meephuch, Y. & Assavamahasakda, C. ( 1986; ). Trypanosomiasis in pigs. In The Sixth Annual Conference of Thailand Livestock Development, Ministry of Agriculture, Bangkok, Thailand.
  10. Shrivastava, K. K. & Shrivastra, G. P. ( 1974; ). Two cases of Trypanosoma species infection of man in India. Trans R Soc Trop Med Hyg 68, 3–4.
    [Google Scholar]
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