1887

Abstract

The multilocus variable-number tandem repeat analysis (MLVA) method to target eight variable-number tandem repeat loci, based on agarose gel electrophoresis separation of multiplexed PCR products, and the PFGE method were applied to clinical isolates of O157 : H7 with the aim of comparing their performance as methods of typing this bacterium. Using MLVA, a total of 57 isolates from patients in Shizuoka prefecture, Japan, were divided into 20 types and classified into 23 PFGE types. Twenty-four isolates from four sporadic infections, four household contact infections and one outbreak that occurred in central parts of Shizuoka prefecture during August to November in 2005 were shown to be the same MLVA type, and most of the isolates had identical PFGE banding patterns, suggesting the diffuse outbreak in these parts of Japan. Thus, there was a good correlation between MLVA types and PFGE types, with both methods displaying broadly similar discriminatory powers. However, the MLVA typing proved to be a much easier and more rapid method for the analysis of O157 : H7 strain relatedness to identify transmission routes. Hence, our MLVA method would be a suitable technique for routine typing in many laboratories, including public health agencies, and even in hospitals.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47213-0
2008-01-01
2019-11-12
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/1/58.html?itemId=/content/journal/jmm/10.1099/jmm.0.47213-0&mimeType=html&fmt=ahah

References

  1. Benson, G. ( 1999; ). Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27, 573–580.[CrossRef]
    [Google Scholar]
  2. Besser, R. E., Griffin, P. M. & Slutsker, L. ( 1999; ). Escherichia coli O157 : H7 gastroenteritis and the hemolytic uremic syndrome: an emerging infectious disease. Annu Rev Med 50, 355–367.[CrossRef]
    [Google Scholar]
  3. Cowan, L. S., Mosher, L., Diem, L., Massey, J. P. & Crawford, J. T. ( 2002; ). Variable-number tandem repeat typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110 by using mycobacterial interspersed repetitive units. J Clin Microbiol 40, 1592–1602.[CrossRef]
    [Google Scholar]
  4. Gupta, A., Hunter, S. B., Bidol, S. A., Dietrich, S., Kincaid, J., Salehi, E., Nichoison, L., Genese, C. A., Todd-Weinstein, S. & other authors ( 2004; ). Escherichia coli O157 cluster evaluation. Emerg Infect Dis 10, 1856–1858.[CrossRef]
    [Google Scholar]
  5. Izumiya, H., Terajima, J., Wada, A., Inagaki, Y., Itoh, K., Tamura, K. & Watanabe, H. ( 1997; ). Molecular typing of enterohemorrhagic Escherichia coli O157 : H7 isolates in Japan by using pulsed-field gel electrophoresis. J Clin Microbiol 35, 1675–1680.
    [Google Scholar]
  6. Keen, J. E., Wittum, T. E., Dunn, J. R., Bono, J. L. & Durso, L. M. ( 2006; ). Shiga-toxigenic Escherichia coli O157 in agricultural fair livestock, United States. Emerg Infect Dis 12, 780–786.[CrossRef]
    [Google Scholar]
  7. Keim, P., Price, L. B., Klevytska, A. M., Smith, K. L., Schupp, J. M., Okinaka, R., Jackson, P. J. & Hugh-Jones, M. E. ( 2000; ). Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis. J Bacteriol 182, 2928–2936.[CrossRef]
    [Google Scholar]
  8. Keys, C., Kemper, S. & Keim, P. ( 2005; ). Highly diverse variable number tandem repeat loci in the E. coli O157 : H7 and O55 : H7 genomes for high-resolution molecular typing. J Appl Microbiol 98, 928–940.[CrossRef]
    [Google Scholar]
  9. Klevytska, A. M., Price, L. B., Schupp, J. M., Worsham, P. L., Wong, J. & Keim, P. ( 2001; ). Identification and characterization of variable-number tandem repeats in the Yersinia pestis genome. J Clin Microbiol 39, 3179–3185.[CrossRef]
    [Google Scholar]
  10. Lindstedt, B. A. ( 2005; ). Multiple-locus variable number tandem repeats analysis for genetic fingerprinting of pathogenic bacteria. Electrophoresis 26, 2567–2582.[CrossRef]
    [Google Scholar]
  11. Lindstedt, B. A., Heir, E., Gjernes, E. & Kapperud, G. ( 2003; ). DNA fingerprinting of Salmonella enterica subsp. enterica serovar Typhimurium with emphasis on phage type DT104 based on variable number of tandem repeat loci. J Clin Microbiol 41, 1469–1479.[CrossRef]
    [Google Scholar]
  12. Lindstedt, B. A., Vardund, T. & Kapperud, G. ( 2004; ). Multiple-locus variable-number tandem-repeats analysis of Escherichia coli O157 using PCR multiplexing and multi-colored capillary electrophoresis. J Microbiol Methods 58, 213–222.[CrossRef]
    [Google Scholar]
  13. Matsumoto, M., Suzuki, Y., Nagano, H., Yatsuyanagi, J., Kurosawa, H., Kobayashi, K., Yamaoka, K., Horikawa, K., Kudaka, J. & other authors ( 2005; ). Evaluation of pulsed-field gel electrophoresis analysis performed at selected prefectural institutes of public health for use in PulseNet Japan. Jpn J Infect Dis 58, 180–183.
    [Google Scholar]
  14. Noller, A. C., McEllistrem, M. C., Pacheco, A. G. F., Boxrud, D. J. & Harrison, L. H. ( 2003; ). Multilocus variable-number tandem repeat analysis distinguishes outbreak and sporadic Escherichia coli O157 : H7 isolates. J Clin Microbiol 41, 5389–5397.[CrossRef]
    [Google Scholar]
  15. Noller, A. C., McEllistrem, M. C., Shutt, K. A. & Harrison, L. H. ( 2006; ). Locus-specific mutational events in a multilocus variable-number tandem repeat analysis of Escherichia coli O157 : H7. J Clin Microbiol 44, 374–377.[CrossRef]
    [Google Scholar]
  16. Parry, S. M. & Salmon, R. L. ( 1998; ). Sporadic STEC O157 infection: secondary household transmission in Wales. Emerg Infect Dis 4, 657–661.[CrossRef]
    [Google Scholar]
  17. Rangel, J. M., Sparling, P. H., Crowe, C., Griffin, P. M. & Swerdlow, L. ( 2005; ). Epidemiology of Escherichia coli O157 : H7 outbreaks, United States, 1982–2002. Emerg Infect Dis 11, 603–609.[CrossRef]
    [Google Scholar]
  18. Ribot, E. M., Fitzgerald, C., Kubota, K., Swaminatham, B. & Barrett, T. J. ( 2001; ). Rapid pulsed-field gel electrophoresis protocol for subtyping of Campylobacter jejuni. J Clin Microbiol 39, 1889–1894.[CrossRef]
    [Google Scholar]
  19. Savine, E., Warren, R. M., van der Spuy, G. D., Beyers, N., van Helden, P. D., Locht, G. & Supply, P. ( 2002; ). Stability of variable-number tandem repeats of mycobacterial interspersed repetitive unit from 12 loci in serial isolates of Mycobacterium tuberculosis. J Clin Microbiol 40, 4561–4566.[CrossRef]
    [Google Scholar]
  20. Swaminathan, B., Barrett, T. J., Hunter, S. B. & Tauxe, R. V., the CDC PulseNet Task Force ( 2001; ). PulseNet: molecular subtyping network for foodborne bacterial disease surveillance, United States. Emerg Infect Dis 7, 382–389.[CrossRef]
    [Google Scholar]
  21. van Belkum, A., Scherer, S., van Alphen, L. & Verbrugh, H. ( 1998; ). Short-sequence DNA repeats in prokaryotic genomes. Microbiol Mol Biol Rev 62, 275–293.
    [Google Scholar]
  22. Watanabe, H., Wada, A., Inagaki, Y., Itoh, K. & Tamura, K. ( 1996; ). Outbreaks of enterohaemorrhagic Escherichia coli O157 : H7 infection by two different genotypes-strains in Japan, 1996. Lancet 348, 831–832.[CrossRef]
    [Google Scholar]
  23. Yazdankhah, S. P., Lindstedt, B. A. & Caugant, D. A. ( 2005; ). Use of variable-number tandem repeats to examine genetic diversity of Neisseria meningitidis. J Clin Microbiol 43, 1699–1705.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47213-0
Loading
/content/journal/jmm/10.1099/jmm.0.47213-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error