1887

Abstract

Epidemiological studies in both humans and animals conducted in Mexico have shown that the isolation frequency of O157 : H7 is low. In a previous study, IgG antibodies against . O157, O7 and O116 LPS were found in serum samples from children and adults with no previous history of . O157 : H7 infection. The present study was designed to determine whether a similar immune response against . O157 : H7 and other antigenically related bacteria was present in bovine serum samples. A total of 310 serum samples from different herds in Mexico was analysed by microagglutination assays against different enterobacterial antigens, including . O157. Microagglutination assays were positive against . O7 (55 %), O116 (76 %) and O157 (36 %), (15 %), serotype Urbana (14 %) and subsp. (40 %). These results were confirmed using a specific ELISA with purified LPS. A positive reaction was observed against the LPS of . O7 (29 %), O116 (12 %) and O157 (22 %), . (4 %), Urbana (13 %) and subsp. (12 %). Serum absorption studies of positive serum samples indicated the existence of at least three common epitopes shared by the LPS of . O7, O116 and O157, and two others between . O157 and Urbana and . subsp. . A bactericidal assay against . O157 : H7 using 31 bovine serum samples was performed, and 22 (71 %) of these serum samples gave positive results. The data demonstrated that bovine serum showed a response against different enterobacteria, including O157, and that this response could be due to the presence of shared epitopes in the LPS of these organisms.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47201-0
2007-11-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/11/1447.html?itemId=/content/journal/jmm/10.1099/jmm.0.47201-0&mimeType=html&fmt=ahah

References

  1. Ahmed, A., Li, J., Shiloach, Y., Robbins, J. B. & Szu, S. C. ( 2006; ). Safety and immunogenicity of Escherichia coli O157 O-specific polysaccharide conjugate vaccine in 2–5-year-old children. J Infect Dis 193, 515–521.[CrossRef]
    [Google Scholar]
  2. Amor, K., Heinrichs, D. E., Frirdich, E., Ziebell, K., Johnson, R. P. & Whitfield, C. ( 2000; ). Distribution of core oligosaccharide types in lipopolysaccharides from Escherichia coli. Infect Immun 68, 1116–1124.[CrossRef]
    [Google Scholar]
  3. Bitzan, M., Ludwig, K., Klemt, M., Konig, H., Buren, J. & Muller-Wiefel, D. E. ( 1993; ). The role of Escherichia coli O157 infections in the classical (enteropathic) haemolytic uraemic syndrome: results of a Central European, multicentre study. Epidemiol Infect 110, 183–196.[CrossRef]
    [Google Scholar]
  4. Bundle, D. R., Gerken, M. & Perry, M. B. ( 1986; ). Two-dimensional nuclear magnetic resonance at 500 MHz: the structural elucidation of Salmonella serogroup N polysaccharide antigen. Can J Chem 64, 255–264.[CrossRef]
    [Google Scholar]
  5. Callaway, T. R., Anderson, R. C., Tellez, G., Rosario, C., Nava, G. M., Eslava, C., Blanco, M. A., Quiroz, M. A., Olguin, A. & other authors ( 2004; ). Prevalence of Escherichia coli O157 in cattle and swine in central Mexico. J Food Prot 67, 2274–2276.
    [Google Scholar]
  6. Caprioli, A., Morabito, S., Brugère, H. & Oswald, E. ( 2005; ). Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission. Vet Res 36, 289–311.[CrossRef]
    [Google Scholar]
  7. Caroff, M. & Karibian, D. ( 2003; ). Structure of bacterial lipopolysaccharides. Carbohydr Res 338, 2431–2447.[CrossRef]
    [Google Scholar]
  8. Cerqueira, A. M. F., Guth, B. E. C., Joaquim, R. M. & Andrade, J. R. C. ( 1999; ). High occurrence of Shiga toxin-producing Escherichia coli (STEC) in healthy cattle in Rio de Janeiro State, Brazil. Vet Microbiol 70, 111–121.[CrossRef]
    [Google Scholar]
  9. Chapman, P. A., Siddons, C. A., Cerdan Malo, A. T. & Harkin, M. A. ( 1997; ). A 1-year study of Escherichia coli O157 in cattle, sheep, pigs and poultry. Epidemiol Infect 119, 245–250.[CrossRef]
    [Google Scholar]
  10. Chart, H. & Rowe, B. ( 1992; ). Improved detection of infection by Escherichia coli O157 in patients with haemolytic uraemic syndrome by means of IgA antibodies to lipopolysaccharide. J Infect 24, 257–261.[CrossRef]
    [Google Scholar]
  11. Chart, H., Scotland, S. M. & Rowe, B. ( 1989; ). Serum antibodies to Escherichia coli O157 : H7 in patients with hemolytic uremic syndrome. J Clin Microbiol 27, 285–290.
    [Google Scholar]
  12. Chart, H., Cheasty, T. & Willshaw, G. A. ( 2002; ). Production of serum antibodies that recognise epitopes located on the R3 region of Escherichia coli core lipopolysaccharides by patients infected with strains of enterohaemorrhagic E. coli. J Med Microbiol 51, 1050–1054.
    [Google Scholar]
  13. Cobbold, R. & Desmarchelier, P. ( 2000; ). A longitudinal study of Shiga-toxigenic Escherichia coli (STEC) prevalence in three Australian diary herds. Vet Microbiol 71, 125–137.[CrossRef]
    [Google Scholar]
  14. Cravioto, A., Reyes, R. E., Trujillo, F., Uribe, F., Navarro, A., De La Roca, J. M., Hernandez, J. M., Perez, G. & Vazquez, V. ( 1990; ). Risk of diarrhea during the first year of life associated with initial and subsequent colonization by specific enteropathogens. Am J Epidemiol 131, 886–904.
    [Google Scholar]
  15. Cray, W. C., Jr & Moon, H. W. ( 1995; ). Experimental infection of calves and adult cattle with Escherichia coli O157 : H7. Appl Environ Microbiol 61, 1586–1590.
    [Google Scholar]
  16. Currie, C. G. & Poxton, I. R. ( 1999; ). The lipopolysaccharide core type of Escherichia coli O157 : H7 and other non-O157 Verotoxin-producing E. coli. FEMS Immunol Med Microbiol 24, 57–62.[CrossRef]
    [Google Scholar]
  17. Currie, C. G., McCallum, K. & Poxton, I. R. ( 2001; ). Mucosal and systemic antibody response to the lipopolysaccharide of Escherichia coli O157 in health and disease. J Med Microbiol 50, 345–354.
    [Google Scholar]
  18. Diez-Gonzalez, F., Callaway, T. R., Kizoulis, M. G. & Russell, J. B. ( 1998; ). Grain feeding and the dissemination of acid-resistant Escherichia coli from cattle. Science 281, 1666–1668.[CrossRef]
    [Google Scholar]
  19. Ewing, W. H. ( 1986; ). Edwards and Ewing's Identification of Enterobacteriaceae. New York: Elsevier.
  20. Faith, N. G., Shere, J. A., Brosch, R., Arnold, K. W., Ansay, S. E., Lee, M. S., Luchansky, J. B. & Kaspar, C. W. ( 1996; ). Prevalence and clonal nature of Escherichia coli O157 : H7 on dairy farms in Wisconsin. Appl Environ Microbiol 62, 1519–1525.
    [Google Scholar]
  21. Gibb, A. P., Barclay, G. R., Poxton, I. R. & di Padova, F. ( 1992; ). Frequencies of lipopolysaccharide core types among clinical isolates of Escherichia coli defined with monoclonal antibodies. J Infect Dis 166, 1051–1057.[CrossRef]
    [Google Scholar]
  22. Gibbs, R. J., Stewart, J. & Poxton, I. R. ( 2004; ). The distribution of, and antibody response to, the core lipopolysaccharide region of Escherichia coli isolated from the faeces of healthy humans and cattle. J Med Microbiol 53, 959–964.[CrossRef]
    [Google Scholar]
  23. Gillespie, I. A., O'Brien, S. J., Adak, G. K., Cheasty, T. & Willshaw, G. ( 2005; ). Foodborne general outbreaks of Shiga toxin-producing Escherichia coli O157 in England and Wales 1992–2002: where are the risks?. Epidemiol Infect 133, 803–808.[CrossRef]
    [Google Scholar]
  24. Greatorex, J. S. & Thorne, G. M. ( 1994; ). Humoral immune response to Shiga-like toxin and Escherichia coli O157 lipopolysaccharide in hemolytic-uremic syndrome patients and healthy subjects. J Clin Microbiol 32, 1172–1178.
    [Google Scholar]
  25. Griffin, P. M. & Tauxe, R. V. ( 1991; ). The epidemiology of infections caused by Escherichia coli O157 : H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev 13, 60–98.
    [Google Scholar]
  26. Hancock, D. D., Besser, T. E., Rice, D. H., Herriott, D. E. & Tarr, P. I. ( 1997; ). A longitudinal study of Escherichia coli O157 in fourteen cattle herds. Epidemiol Infect 118, 193–195.[CrossRef]
    [Google Scholar]
  27. Irino, K., Kato, M. A. M. F., Vaz, T. M. I., Ramos, I. I., Souza, M. A. C., Cruz, A. S., Gomes, T. A. T., Vieira, M. A. M. & Guth, B. E. C. ( 2005; ). Serotypes and virulence markers of Shiga toxin-producing Escherichia coli (STEC) isolated from dairy cattle in Săo Pablo State, Brazil. Vet Microbiol 105, 29–36.[CrossRef]
    [Google Scholar]
  28. Kenne, L., Lindberg, B., Unger, P., Gustafsson, B. & Holme, T. ( 1982; ). Structural studies of the Vibrio cholerae O-antigen. Carbohydr Res 100, 341–349.[CrossRef]
    [Google Scholar]
  29. Konadu, E., Robbins, J. B., Schiloach, J., Bryla, D. A. & Szu, S. C. ( 1994; ). Preparation, characterization, and immunological properties in mice of Escherichia coli O157 O-specific polysaccharide-protein conjugate vaccine. Infect Immun 62, 5048–5054.
    [Google Scholar]
  30. Konadu, E. Y., Parke, J. C., Jr, Tran, H. T., Bryla, D. A., Robbins, J. B. & Szu, S. C. ( 1998; ). Investigational vaccine for Escherichia coli O157: phase 1 study of O157 O-specific polysaccharide–Pseudomonas aeruginosa recombinant exoprotein A conjugate in adults. J Infect Dis 177, 383–387.[CrossRef]
    [Google Scholar]
  31. Laegreid, W. W., Elder, R. O. & Keen, J. E. ( 1999; ). Prevalence of Escherichia coli O157 : H7 in range beef calves at weaning. Epidemiol Infect 123, 291–298.[CrossRef]
    [Google Scholar]
  32. Mattar, S. & Vásquez, E. ( 1998; ). Escherichia coli O157 : H7 infection in Colombia. Emerg Infect Dis 4, 126–127.[CrossRef]
    [Google Scholar]
  33. Matthews, L., Low, J. C., Gally, D. L., Pearce, M. C., Mellor, D. J., Heesterbeek, J. A. P., Chase-Topping, M., Naylor, S. W., Shaw, D. J. & other authors ( 2006; ). Heterogeneous shedding of Escherichia coli O157 in cattle and its implications for control. Proc Natl Acad Sci U S A 103, 547–552.[CrossRef]
    [Google Scholar]
  34. Meichtri, L., Miliwebsky, E., Gioffré, A., Chinen, I., Baschkier, A., Chillemi, G., Guth, B. E. C., Masana, M. O., Cataldi, A. & other authors ( 2004; ). Shiga toxin-producing Escherichia coli in healthy young beef steers from Argentina: prevalence and virulence properties. Int J Food Microbiol 96, 189–198.[CrossRef]
    [Google Scholar]
  35. Miyao, Y., Kataoka, T., Nomoto, T., Kai, A., Itoh, T. & Itoh, K. ( 1998; ). Prevalence of verotoxin-producing Escherichia coli harbored in the intestine of cattle in Japan. Vet Microbiol 61, 137–143.[CrossRef]
    [Google Scholar]
  36. Navarro, A., Eslava, C., Hernandez, U., Navarro-Henze, J. L., Avilés, M., Garcia de la Torre, G. & Cravioto, A. ( 2003; ). Antibody response to Escherichia coli O157 and other lipopolysaccharides in healthy children and adults. Clin Diagn Lab Immunol 10, 797–801.
    [Google Scholar]
  37. Navarro, A., Eslava, C., Gutierrez, A., Licona, D., León, L., Pérez, G., Díaz, M., Díaz, S. P., Uribe, M. & other authors ( 2006; ). Immune response to Escherichia coli O157 LPS in dairy cattle in Mexico is not associated with intestinal colonization by Escherichia coli O157 strains. In Abstracts of the 6th International Symposium on Shiga Toxin (Verocytotoxin)-producing Escherichia coli infections (VTEC 2006 Melbourne), Melbourne, Australia,. abstract P06.01.04.
  38. Naylor, S. W., Gally, D. L. & Low, J. C. ( 2005; ). Enterohaemorrhagic E. coli in veterinary medicine. Int J Med Microbiol 295, 419–441.[CrossRef]
    [Google Scholar]
  39. Nishiuchi, Y., Doe, M., Hotta, H. & Kobayashi, K. ( 2000; ). Structure and serologic properties of O-specific polysaccharide from Citrobacter freundii possessing cross-reactivity with Escherichia coli O157 : H7. FEMS Immunol Med Microbiol 28, 163–171.[CrossRef]
    [Google Scholar]
  40. Omisakin, F., MacRae, M., Ogden, I. D. & Strachan, N. J. C. ( 2003; ). Concentration and prevalence of Escherichia coli O157 in cattle feces at slaughter. Appl Environ Microbiol 69, 2444–2447.[CrossRef]
    [Google Scholar]
  41. Ørskov, F. & Ørskov, I. ( 1984; ). Serotyping of Escherichia coli. Meth Microbiol 14, 43–112.
    [Google Scholar]
  42. Padola, N. L., Sanz, M. E., Blanco, J. E., Blanco, M., Blanco, J., Etcheverria, A. I., Arroyo, G. H., Usera, M. A. & Parma, A. E. ( 2004; ). Serotypes and virulence of bovine Shigatoxigenic Escherichia coli (STEC) isolated from a feedlot in Argentina. Vet Microbiol 100, 3–9.[CrossRef]
    [Google Scholar]
  43. Paiba, G. A., Wilesmith, J. W., Evans, S. J., Pascoe, S. J. S., Smith, R. P., Kidd, S. A., Ryan, J. B. M., McLaren, I. M., Chappell, S. A. & other authors ( 2003; ). Prevalence of faecal excretion of Verocytotoxigenic Escherichia coli O157 in cattle in England and Wales. Vet Rec 153, 347–353.[CrossRef]
    [Google Scholar]
  44. Perry, M. B. & Bundle, D. R. ( 1990; ). Antigenic relationships of the lipopolysaccharides of Escherichia hermannii strains with those of Escherichia coli O157 : H7, Brucella melitensis, and Brucella abortus. Infect Immun 58, 1391–1395.
    [Google Scholar]
  45. Perry, M. B., MacLean, L. & Griffith, D. W. ( 1986; ). Structure of O-chain polysaccharide of the phenol-phase soluble lipopolysaccharide of Escherichia coli O157 : H7. Biochem Cell Biol 64, 21–28.[CrossRef]
    [Google Scholar]
  46. Qadri, F., Mohi, G., Hossain, J., Azim, T., Khan, A. M., Salam, M. A., Sack, R. B., Albert, M. J. & Svennerholm, A.-M. ( 1995; ). Comparison of the vibriocidal antibody response in cholera due to Vibrio cholerae O139 Bengal with the response in cholera due to Vibrio cholerae O1. Clin Diagn Lab Immunol 2, 685–688.
    [Google Scholar]
  47. Rangel, J. M., Sparling, P. H., Crowe, C., Griffin, P. M. & Swerdlow, D. L. ( 2005; ). Epidemiology of Escherichia coli O157 : H7 outbreaks, United States, 1982–2002. Emerg Infect Dis 11, 603–609.[CrossRef]
    [Google Scholar]
  48. Rice, E. W., Sowers, E. G., Johnson, C. H., Dunnigan, M. E., Strockbine, N. A. & Edberg, S. C. ( 1992; ). Serological cross-reactions between Escherichia coli O157 and other species of the genus Escherichia. J Clin Microbiol 30, 1315–1316.
    [Google Scholar]
  49. Samuel, G., Hogbin, J.-P., Wang, L. & Reeves, P. R. ( 2004; ). Relationships of the Escherichia coli O157, O111, and O55 O-antigen gene cluster with those of Salmonella enterica and Citrobacter freundii, which express identical O antigens. J Bacteriol 186, 6536–6543.[CrossRef]
    [Google Scholar]
  50. Smith, D., Blackford, M., Younts, S., Moxley, R., Gray, J., Hungerford, L., Milton, T. & Klopfenstein, T. ( 2001; ). Ecological relationship between the prevalence of cattle shedding Escherichia coli O157 : H7 and characteristics of the cattle or conditions of the feedlot pen. J Food Prot 64, 1899–1903.
    [Google Scholar]
  51. Varela-Hernández, J. J., Cabrera-Diaz, E., Cardona-López, M. A., Ibarra-Velázquez, L. M., Rangel-Villalobos, H., Castillo, A., Torres-Vitela, M. R. & Ramírez-Alvarez, A. ( 2007; ). Isolation and characterization of Shiga toxin-producing Escherichia coli O157 : H7 and non-O157 from beef carcasses at a slaughter plant in Mexico. Int J Food Microbiol 113, 237–241.[CrossRef]
    [Google Scholar]
  52. Westphal, O. & Jann, K. ( 1965; ). Bacterial lipopolysaccharide: extraction with phenol-water and further applications of the procedure. Meth Carbohydr Chem 5, 83–91.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47201-0
Loading
/content/journal/jmm/10.1099/jmm.0.47201-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error