1887

Abstract

The aim of this study was to evaluate an immunoassay for the detection of human serum antibodies to the LPS and flagellar antigens of Typhi and Paratyphi A, B and C, and to the Vi capsular polysaccharide of Typhi and Paratyphi C. A total of 330 sera were used; these originated from 15 patients who were culture-positive for Typhi and 15 healthy controls, together with 300 sera submitted to the Laboratory of Enteric Pathogens for serodiagnosis. By SDS-PAGE/immunoblotting, all 15 sera from culture-positive patients had serum antibodies to the 9,12 LPS antigens and 10 had antibodies to the ‘d’ flagellar antigens. Of the 300 reference sera, 22 had antibodies to the 9,12 LPS antigens, one to the 1,4,5,12 LPS antigens and 12 to the 6,7 LPS antigens. Only two sera had antibodies to flagellar antigens, one of which bound to the ‘b’ and the other to the ‘d’ antigen. An ELISA was developed that successfully detected serum antibodies to the Vi capsular polysaccharides, but because of the kinetics of serum antibody production to the Vi, these antibodies may be of limited value in the serodiagnosis of acute infection with Typhi and Paratyphi C. The immunoassays described here provide a sensitive means of detecting serum antibodies to the LPS, flagellar and Vi antigens of Typhi and Paratyphi, and constitute a viable replacement for the Widal assay for the screening of sera. The serodiagnosis protocols described here are the new standard operating procedures used by the Health Protection Agency's National Salmonella Reference Centre based in the Laboratory of Enteric Pathogens, Colindale, UK.

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2007-09-01
2019-11-12
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References

  1. Anonymous ( 1961; ). The detection of the typhoid carrier state. J Hyg (Lond) 59, 231–247.[CrossRef]
    [Google Scholar]
  2. Anonymous ( 1978; ). Typhoid and its serology. Br Med J 1, 389–390.
    [Google Scholar]
  3. Bhatnagar, S. S. ( 1938; ). Vi agglutination in the diagnosis of typhoid fever and typhoid carrier condition. Br Med J 2, 1195–1198.[CrossRef]
    [Google Scholar]
  4. Bhatnagar, S. S., Speechly, C. G. J. & Singh, M. A. ( 1938; ). Vi variant of Salmonella typhi and its application to the serology of typhoid fever. J Hyg (Lond) 38, 663–672.[CrossRef]
    [Google Scholar]
  5. Brodie, J. ( 1977; ). Antibodies and the Aberdeen typhoid outbreak of 1964 I: the Widal reaction. J Hyg (Lond) 79, 161–180.[CrossRef]
    [Google Scholar]
  6. Buck, R. L., Escamilla, J., Sangalang, R. P., Cabanban, A. B., Santiago, L. T., Ranoa, C. P. & Cross, J. H. ( 1987; ). Diagnostic value of a single, pre-treatment Widal test in suspected enteric fever cases in the Philippines. Trans R Soc Trop Med Hyg 81, 871–873.[CrossRef]
    [Google Scholar]
  7. Chart, H., Scotland, S. M. & Rowe, B. ( 1989; ). Serum antibodies to Escherichia coli serotype O157 : H7 in patients with hemolytic uremic syndrome. J Clin Microbiol 27, 285–290.
    [Google Scholar]
  8. Chart, H., Ward, L. R. & Rowe, B. ( 1995; ). Serological response of patients with clinical typhoid. Serodiagn Immunother Infect Dis 7, 30–33.[CrossRef]
    [Google Scholar]
  9. Chart, H., Rowe, B. & Cheesbrough, J. S. ( 1997; ). The serological response of patients infected with Salmonella typhi. J Clin Pathol 50, 944–946.[CrossRef]
    [Google Scholar]
  10. Chart, H., Cheesbrough, J. S. & Waghorn, D. J. ( 2000; ). The serodiagnosis of infection with Salmonella typhi. J Clin Pathol 53, 851–853.[CrossRef]
    [Google Scholar]
  11. Dutta, S., Sur, D., Manna, B., Sen, B., Deb, A. K., Deen, J. L., Wain, J., Von Seidlein, L., Ochiai, L. & other authors ( 2006; ). Evaluation of new-generation serologic tests for the diagnosis of typhoid fever: data from a community-based surveillance in Calcutta, India. Diagn Microbiol Infect Dis 56, 359–365.[CrossRef]
    [Google Scholar]
  12. Felix, A. ( 1950; ). Standardization of diagnostic agglutination tests. Bull World Health Organ 2, 643–649.
    [Google Scholar]
  13. Felix, A. & Bensted, H. J. ( 1954; ). Proposed standard agglutinating sera for typhoid and paratyphoid A and B fevers. Bull World Health Organ 10, 919–926.
    [Google Scholar]
  14. Felix, A. & Petrie, F. ( 1938; ). The preparation of anti-typhoid serum in the horse for therapeutic use in man. J Hyg (Lond) 38, 673–682.[CrossRef]
    [Google Scholar]
  15. Felix, A. & Pitt, M. R. ( 1951; ). The pathogenic and immunogenic activities of Salmonella typhi in relation to its antigenic constituents. J Hyg (Lond) 49, 92–110.[CrossRef]
    [Google Scholar]
  16. Felix, A., Krikorian, K. S. & Reiter, R. ( 1935; ). The occurrence of typhoid bacilli containing Vi antigen in cases of typhoid fever and Vi antibody in their sera. J Hyg (Lond) 35, 421–427.[CrossRef]
    [Google Scholar]
  17. Glauer, D. & Richter, W. ( 1952; ). Vi agglutinin in typhoid fever. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg 158, 80–87 (in German).
    [Google Scholar]
  18. Gupta, A., My Thanh, N. T., Olsen, S. J., Sivapalasingam, S., My Trinh, T. T., Phuong Lan, N. T., Hoekstra, R. M., Bibb, W., Minh, N. T. & other authors ( 2006; ). Evaluation of community-based serologic screening for identification of chronic Salmonella typhi carriers in Vietnam. Int J Infect Dis 10, 309–314.[CrossRef]
    [Google Scholar]
  19. Hessel, L., Debois, H., Fletcher, M. & Dumas, R. ( 1999; ). Experience with Salmonella typhi Vi vaccine. Eur J Clin Microbiol Infect Dis 18, 609–620.[CrossRef]
    [Google Scholar]
  20. Hoffman, S. L., Flanigan, T. P., Klaucke, D., Leksana, B., Rockhill, R. C., Punjabi, N. H., Pulungsih, S. P., Sutomo, A. & Moechtar, M. A. ( 1986; ). The Widal slide agglutination test, a valuable rapid diagnostic test in typhoid fever patients at the Infectious Diseases Hospital of Jakarta. Am J Epidemiol 123, 869–875.
    [Google Scholar]
  21. House, D., Chinh, N. T., Diep, T. S., Parry, C. M., Wain, J., Dougan, G., White, N. J., Hien, T. T. & Farrar, J. J. ( 2005; ). Use of paired serum samples for serodiagnosis of typhoid fever. J Clin Microbiol 43, 4889–4890.[CrossRef]
    [Google Scholar]
  22. Huckstep, R. L. ( 1962; ). Typhoid Fever and Other Salmonella Infections. Edinburgh & London: E. & S. Livingstone.
  23. Koeleman, J. G. M., Regensburg, D. F., van Katwijk, F. & MacLaren, D. M. ( 1992; ). Retrospective study to determine the diagnostic value of the Widal test in a non-endemic country. Eur J Clin Microbiol Infect Dis 11, 167–170.[CrossRef]
    [Google Scholar]
  24. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  25. Lanata, C. F., Levine, M. M., Ristori, C., Black, R. E., Jimenez, L., Salcedo, M., Garcia, J. & Sotomayor, V. ( 1983; ). Vi serology in detection of chronic Salmonella typhi carriers in an endemic area. Lancet ii, 441–443.
    [Google Scholar]
  26. Levine, M. M., Grados, O., Gilman, R. H., Woodward, W. E., Solis-Plaza, R. & Waldman, W. ( 1978; ). Diagnostic value of the Widal test in areas endemic for typhoid fever. Am J Trop Med Hyg 27, 795–800.
    [Google Scholar]
  27. Levine, M. M., Ferreccio, C., Black, R. E., Tacket, C. O. & Germanier, R. ( 1989; ). Progress in vaccines against typhoid fever. Rev Infect Dis 11, S552–S567.[CrossRef]
    [Google Scholar]
  28. Lin, F. Y., Becke, J. M., Groves, C., Lim, B. P., Israel, E., Becker, E. F., Helfrich, R. M., Swetter, D. S., Cramton, T. & Robbins, J. B. ( 1988; ). Restaurant-associated outbreak of typhoid fever in Maryland: identification of carrier facilitated by measurement of serum Vi antibodies. J Clin Microbiol 26, 1194–1197.
    [Google Scholar]
  29. Losonsky, G. A., Ferreccio, C., Kotloff, K. L., Kaintuck, S., Robbins, J. B. & Levine, M. M. ( 1987; ). Development and evaluation of an enzyme-linked immunosorbent assay for serum Vi antibodies for detection of chronic Salmonella typhi carriers. J Clin Microbiol 25, 2266–2269.
    [Google Scholar]
  30. Olopoenia, L. A. & King, A. L. ( 2000; ). Widal agglutination test – 100 years later: still plagued by controversy. Postgrad Med J 76, 80–84.[CrossRef]
    [Google Scholar]
  31. Pang, T. & Puthucheary, S. D. ( 1983; ). Significance and value of the Widal test in the diagnosis of typhoid fever in an endemic area. J Clin Pathol 36, 471–475.[CrossRef]
    [Google Scholar]
  32. Parry, C. M. ( 2004; ). Typhoid fever. Curr Infect Dis Rep 6, 27–33.[CrossRef]
    [Google Scholar]
  33. Reynolds, D. W., Carpenter, R. L. & Simon, W. H. ( 1970; ). Diagnostic specificity of Widal's reaction for typhoid fever. JAMA 214, 2192–2193.[CrossRef]
    [Google Scholar]
  34. Robbins, J. D. & Robbins, J. B. ( 1984; ). Reexamination of the protective role of the capsular polysaccharide (Vi antigen) of Salmonella typhi. J Infect Dis 150, 436–449.[CrossRef]
    [Google Scholar]
  35. Rowe, B. & Hall, M. L. M. ( 1989; ). Kauffmann–White Scheme 1989. London: Public Health Laboratory Service Publication.
  36. Schroeder, S. A. ( 1968; ). Interpretation of the serologic tests for typhoid fever. JAMA 206, 839–840.[CrossRef]
    [Google Scholar]
  37. Tacket, C. O., Losonsky, G., Taylor, D. N., Baron, L. S., Kopecko, D., Cryz, S. & Levine, M. M. ( 1991; ). Lack of immune response to the Vi component of a Vi-positive variant of the Salmonella typhi live oral vaccine strain Ty21a in human studies. J Infect Dis 163, 901–904.[CrossRef]
    [Google Scholar]
  38. Tsai, C.-M. & Frasch, C. E. ( 1982; ). A sensitive silver stain for detecting lipopolysaccharide in polyacrylamide gels. Anal Biochem 119, 115–119.[CrossRef]
    [Google Scholar]
  39. Widal, F. ( 1896; ). Sero-diagnosis of typhoid fever. Bull Soc Med Hop Paris 13, 561–566 (in French).
    [Google Scholar]
  40. Zuerlein, T. J. & Smith, P. W. ( 1985; ). The diagnostic utility of the febrile agglutinin tests. JAMA 254, 1211–1214.[CrossRef]
    [Google Scholar]
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