1887

Abstract

-specific nucleotide sequences encoding the BCSP 31 kDa protein, Omp2 and the 16S rRNA were employed in three independent diagnostic PCR assays. Results of the three PCR assays on six reference strains of were in complete agreement. The results of PCR assays based on and on 19 Indian field isolates (human, bovine and murine tissues) also agreed completely. However, when the 16S rRNA gene was employed as the diagnostic target in the PCR, only 14 out of these 19 isolates and 2 out of 7 bovine milk isolates were identified as the genus . The bovine blood samples were insensitive to 16S rRNA PCR. The antibody-detecting ELISA results of field samples (=87) from a serologically positive herd in India were compared separately with and PCRs of blood (=62). While the PCR was the most sensitive, the degree of association of ELISA with blood PCR (=0.37 at <0.05) was similar to that with the blood PCR ( =0.34 at <0.05). An improvement in the correlation between ELISA and blood PCR was noticed ( =0.5 at <0.05) when a consensus result of and blood PCR was considered for comparison with ELISA. The use of more than one marker-based PCR gave increased sensitivity and higher specificity and appears to be a more reliable molecular diagnostic approach for screening of field animals.

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2007-10-01
2019-11-22
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