1887

Abstract

is a pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis. The complexity of mechanisms involved in dermatophytic infections makes relevant studies particularly difficult to perform. The aim of this study was to develop a new model of dermatophytosis using feline fetal keratinocytes in reconstructed interfollicular epidermis, and to investigate its relevance in studying the host–pathogen relationship. Histological analysis of reconstructed interfollicular feline epidermis (RFE) revealed a fully differentiated epidermis. A proliferation assay showed replicating cells only in the basal layer, indicating that RFE is a well-stratified living tissue, leading to the formation of a horny layer. Histopathological analysis of RFE infected by arthroconidia revealed that the fungus invades the stratum corneum and produces SUB3, a keratinase implicated in the infectious process. In view of these results, an dermatophytosis model on RFE seems to be a useful tool to investigate mechanisms involved in natural feline infections.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47115-0
2007-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/7/971.html?itemId=/content/journal/jmm/10.1099/jmm.0.47115-0&mimeType=html&fmt=ahah

References

  1. Chen T. A., Halliwell R. E., Hill P. B. 2002; Failure of extracts from Malassezia pachydermatis to stimulate canine keratinocyte proliferation in vitro. . Vet Dermatol 13:323–329 [CrossRef]
    [Google Scholar]
  2. Descamps F., Brouta F., Monod M., Zaugg C., Baar D., Losson B., Mignon B. 2002; Isolation of a Microsporum canis gene family encoding three subtilisin-like proteases expressed in vivo . J Invest Dermatol 119:830–835 [CrossRef]
    [Google Scholar]
  3. Gupta A. K., Ahmad I., Porretta M., Summerbell R. C. 2003; Arthroconidial formation in Trichophyton raubitschekii . Mycoses 46:322–328
    [Google Scholar]
  4. Mignon B., Swinnen M., Bouchara J. P., Hofinger M., Nikkels A., Pierard G., Gerday C., Losson B. 1998; Purification and characterization of a 31.5 kDa keratinolytic subtilisin-like serine protease from Microsporum canis and evidence of its secretion in naturally infected cats. Med Mycol 36:395–404 [CrossRef]
    [Google Scholar]
  5. Mignon B. R., Leclipteux T., Focant C., Nikkels A. J., Pierard G. E., Losson B. J. 1999; Humoral and cellular immune response to a crude exo-antigen and purified keratinase of Microsporum canis in experimentally infected guinea pigs. Med Mycol 37:123–129 [CrossRef]
    [Google Scholar]
  6. Nakamura Y., Kano R., Hasegawa A., Watanabe S. 2002; Interleukin-8 and tumor necrosis factor alpha production in human epidermal keratinocytes induced by Trichophyton mentagrophytes . Clin Diagn Lab Immunol 9:935–937
    [Google Scholar]
  7. Poumay Y., Dupont F., Marcoux S., Leclercq-Smekens M., Herin M., Coquette A. 2004; A simple reconstructed human epidermis: preparation of the culture model and utilization in in vitro studies. Arch Dermatol Res 296:203–211 [CrossRef]
    [Google Scholar]
  8. Rashid A., Edward M., Richardson M. D. 1995; Activity of terbinafine on Trichophyton mentagrophytes in a human living skin equivalent model. J Med Vet Mycol 33:229–233 [CrossRef]
    [Google Scholar]
  9. Zurita J., Hay R. J. 1987; Adherence of dermatophyte microconidia and arthroconidia to human keratinocytes in vitro . J Invest Dermatol 89:529–534 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47115-0
Loading
/content/journal/jmm/10.1099/jmm.0.47115-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error