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Volume 56, Issue 9

Susceptibility of gnotobiotic transgenic mice (Tgϵ26) with combined deficiencies in natural killer cells and T cells to wild-type and hyphal signalling-defective mutants of Free

Abstract

Germfree transgenic epsilon 26 mice (Tgϵ26), deficient in natural killer cells and T cells, were colonized (alimentary tract) with wild-type or each of two hyphal transcription factor signalling mutant strains (, ). Each strain colonized the alimentary tract, infected keratinized gastric tissues to a similar extent, and induced a granulocyte-dominated inflammatory response in infected tissues. Both wild-type and mutant strains formed hyphae and were able to elicit an increase in cytokine [tumour necrosis factor alpha, interleukin (IL)-10 and IL-12] and chemokine (KC and macrophage inflammatory protein-2] mRNAs in infected tissues; however, administration of the wild-type strain was lethal for the Tgϵ26 mice, whereas the mice colonized with the mutant strains survived. Death of the Tgϵ26-colonized mice appeared to be due to occlusive oesophageal candidiasis, and not to disseminated candidiasis of endogenous origin. In contrast, the mutant strains exhibited a significantly reduced capacity to infect (frequency and severity) oro-oesophageal (tongue and oesophagus) tissues. Therefore, the two hyphal signalling-defective mutants were less able to infect oro-oesophageal tissues and were non-lethal, but retained their ability to colonize the alimentary tract with yeast and hyphae, infect keratinized gastric tissues, and evoke an inflammatory response in orogastric tissues.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): GF, germfree , IL, interleukin , MIP, macrophage inflammatory protein , NK, natural killer , RNI, reactive nitrogen intermediates , ROI, reactive oxygen intermediates and TNFα, tumour necrosis factor alpha
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2007-09-01
2024-12-08
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Susceptibility of gnotobiotic transgenic mice (Tgϵ26) with combined deficiencies in natural killer cells and T cells to wild-type and hyphal signalling-defective mutants of Candida albicans
J. Med. Microbiol. 56, 1138 (2007); https://doi.org/10.1099/jmm.0.47110-0
/content/journal/jmm/10.1099/jmm.0.47110-0
/content/journal/jmm/10.1099/jmm.0.47110-0
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