1887

Abstract

Fine-scale genotyping methods are necessary in order to identify possible sources of human exposure to opportunistic pathogens belonging to the complex (MAC). In this study, amplified fragment length polymorphism (AFLP) analysis was evaluated for fingerprinting 159 patient and environmental MAC isolates from southern California. AFLP analysis accurately identified strains belonging to and and differentiated between strains within each species. The method was also able to differentiate strains that were presumed to be genetically identical in two previous studies using large RFLP analysis with PFGE, or PCR-amplification of DNA segments located between insertion sequences IS and IS. For , drinking-water isolates clustered more closely with each other than with patient or food isolates. Patient isolates were more genetically diverse. None of the environmental isolates shared identical AFLP patterns with patient isolates for either species. There were, however, environmental isolates that shared identical patterns, and patient isolates that shared identical patterns. A subset of the isolates, which are referred to as MX isolates due to their ambiguous identification with the Gen-Probe system, produced AFLP patterns similar to those obtained from isolates. Sequence analysis of 16S rDNA obtained from the MX isolates suggests that they are strains of that were not correctly identified by the AccuProbe from Gen-Probe.

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2007-09-01
2019-11-18
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Description of MAC strains used in this study, including specific source and date of isolation. [ PDF file] (31 KB)

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Peaks (in bp) for each strain (rows 5 - 114) and conversion to presence/absence data (rows 117-226). [ PDF file] (921 KB)

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Peaks (in bp) for each and MX strain (rows 5 - 72) and conversion to presence/absence data (rows 75-142). [ PDF file] (480 KB)

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