1887

Abstract

The performance of fatty acid profiling for strain differentiation of was assessed. Total fatty acids were isolated from the yeast-phase cells of seven stock and two previously unreported clinical strains of var. , as well as from one unreported clinical strain and one stock strain of var. , and one strain of each of three other dimorphic zoopathogenic fungal species, , and . Different colony morphology and pigmentation types of the strains were also included. The most frequently occurring fatty acids were oleic, palmitic, stearic and linoleic acids. There were variations in the relative percentage fatty acid contents of strains that could be used for strain identification and discrimination. Differentiation between strains was achieved by the comparison of detected fatty acids accompanied by principal component analysis using calculated Varimax-rotated principal component loadings. Statistical analysis yielded three major principal components that explained over 94 % of total variance in the data. All the strains of var. RFLP classes II and III were grouped into two distinct clusters: the heterogenic RFLP class I formed a large, but also well-defined group, whereas the outgroup strains of var. , , and were shifted away. These data suggest that fatty acid profiling can be used in strain classification and epidemiological studies that require strain differentiation at the intraspecies level.

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2007-06-01
2019-11-14
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