1887

Abstract

Serogroup classifications based upon the O-somatic antigen of Shiga toxin-producing (STEC) provide significant epidemiological information on clinical isolates. Each O-antigen determinant is encoded by a unique cluster of genes present between the and chromosomal genes. Alternatively, serogroup-specific polymorphisms might be encoded in loci that are encoded outside of the O-antigen gene cluster. Segments of the core bacterial loci , , , , , , and for 30 O26 STEC strains have previously been sequenced, and comparative analyses to O157 distinguished these two serogroups. To screen these loci for serogroup-specific traits within a broader range of clinically significant serogroups, DNA sequences were obtained for 19 strains of 10 additional STEC serogroups. Unique alleles were observed at the locus for each examined STEC serogroup, and this correlation persisted when comparative analyses were extended to 144 sequences from 26 O-serogroups (comprising 42 O : H-serotypes). These included O157, O121, O103, O26, O5 : non-motile (NM), O145 : NM, O113 : H21, O111 : NM and O117 : H7 STEC; and furthermore, non-toxin encoding O157, O26, O55, O6 and O117 strains encoded distinct alleles compared to STEC strains of the same serogroup. DNA sequencing of a 643 bp region of was, therefore, sufficient to minimally determine the O-antigen of STEC through molecular means, and the location of next to the O-antigen gene cluster offered additional support for the co-inheritance of these determinants. The DNA sequence-based serogrouping method could improve the typing capabilities for STEC in clinical laboratories, and was used successfully to characterize O121 : H19, O26 : H11 and O177 : NM clinical isolates prior to serological confirmation during outbreak investigations.

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2007-05-01
2019-11-14
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References

  1. Adiri, R. S., Gophna, U. & Ron, E. Z. ( 2003; ). Multilocus sequence typing (MLST) of Escherichia coli O78 strains. FEMS Microbiol Lett 222, 199–203.[CrossRef]
    [Google Scholar]
  2. Beutin, L., Kaulfuss, S., Herold, S., Oswald, E. & Schmidt, H. ( 2005; ). Genetic analysis of enteropathogenic and enterohemorrhagic Escherichia coli serogroup O103 strains by molecular typing of virulence and housekeeping genes and pulsed-field gel electrophoresis. J Clin Microbiol 43, 1552–1563.[CrossRef]
    [Google Scholar]
  3. Bisercic, M., Feutrier, J. Y. & Reeves, P. R. ( 1991; ). Nucleotide sequences of the gnd genes from nine natural isolates of Escherichia coli: evidence of intragenic recombination as a contributing factor in the evolution of the polymorphic gnd locus. J Bacteriol 173, 3894–3900.
    [Google Scholar]
  4. Coimbra, R. S., Grimont, F., Lenormand, P., Burguiere, P., Beutin, L. & Grimont, P. A. ( 2000; ). Identification of Escherichia coli O-serogroups by restriction of the amplified O-antigen gene cluster (rfb-RFLP). Res Microbiol 151, 639–654.[CrossRef]
    [Google Scholar]
  5. DebRoy, C., Roberts, E., Kundrat, J., Davis, M. A., Briggs, C. E. & Fratamico, P. M. ( 2004; ). Detection of Escherichia coli serogroups O26 and O113 by PCR amplification of the wzx and wzy genes. Appl Environ Microbiol 70, 1830–1832.[CrossRef]
    [Google Scholar]
  6. D'Souza, J. M., Wang, L. & Reeves, P. ( 2002; ). Sequence of the Escherichia coli O26 O antigen gene cluster and identification of O26 specific genes. Gene 297, 123–127.[CrossRef]
    [Google Scholar]
  7. DebRoy, C., Fratamico, P. M., Roberts, E., Davis, M. A. & Liu, Y. ( 2005; ). Development of PCR assays targeting genes in O-antigen gene clusters for detection and identification of Escherichia coli O45 and O55 serogroups. Appl Environ Microbiol 71, 4919–4924.[CrossRef]
    [Google Scholar]
  8. Dykhuizen, D. E. & Green, L. ( 1991; ). Recombination in Escherichia coli and the definition of biological species. J Bacteriol 173, 7257–7268.
    [Google Scholar]
  9. Ewing, W. H. ( 1986; ). The genus Escherichia. In Edwards & Ewing’s Identification of Enterobacteriaceae, 4th edn, pp. 93–134. Edited by P. R. Edwards and W. H. Ewing. New York: Elsevier.
  10. Fey, P. D., Wickert, R. S., Rupp, M. E., Safranek, T. J. & Hinrichs, S. H. ( 2000; ). Prevalence of non-O157 : H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska. Emerg Infect Dis 6, 530–533.[CrossRef]
    [Google Scholar]
  11. Fratamico, P. M., DebRoy, C., Strobaugh, T. P., Jr & Chen, C. Y. ( 2005; ). DNA sequence of the Escherichia coli O103 O antigen gene cluster and detection of enterohemorrhagic E. coli O103 by PCR amplification of the wzx and wzy genes. Can J Microbiol 51, 515–522.[CrossRef]
    [Google Scholar]
  12. Gilmour, M. W., Cote, T., Munro, J., Chui, L., Wylie, J., Isaac-Renton, J., Horsman, G., Tracz, D. M., Andrysiak, A. & Ng, L. K. ( 2005; ). Multilocus sequence typing of Escherichia coli O26 : H11 isolates carrying stx in Canada does not identify genetic diversity. J Clin Microbiol 43, 5319–5323.[CrossRef]
    [Google Scholar]
  13. Gilmour, M. W., Tracz, D. M., Andrysiak, A. K., Clark, C. G., Tyson, S., Severini, A. & Ng, L. K. ( 2006; ). Use of the espZ gene encoded in the locus of enterocyte effacement for molecular typing of Shiga toxin-producing Escherichia coli. J Clin Microbiol 44, 449–458.[CrossRef]
    [Google Scholar]
  14. Girardeau, J. P., Dalmasso, A., Bertin, Y., Ducrot, C., Bord, S., Livrelli, V., Vernozy-Rozand, C. & Martin, C. ( 2005; ). Association of virulence genotype with phylogenetic background in comparison to different seropathotypes of Shiga toxin-producing Escherichia coli isolates. J Clin Microbiol 43, 6098–6107.[CrossRef]
    [Google Scholar]
  15. Hsu, C. F., Tsai, T. Y. & Pan, T. M. ( 2005; ). Use of the duplex TaqMan PCR system for detection of Shiga-like toxin-producing Escherichia coli O157. J Clin Microbiol 43, 2668–2673.[CrossRef]
    [Google Scholar]
  16. Huson, D. H. ( 1998; ). SplitsTree: analyzing and visualizing evolutionary data. Bioinformatics 14, 68–73.[CrossRef]
    [Google Scholar]
  17. Jelacic, J. K., Damrow, T., Chen, G. S., Jelacic, S., Bielaszewska, M., Ciol, M., Carvalho, H. M., Melton-Celsa, A. R., O'Brien, A. D. & Tarr, P. I. ( 2003; ). Shiga toxin-producing Escherichia coli in Montana: bacterial genotypes and clinical profiles. J Infect Dis 188, 719–729.[CrossRef]
    [Google Scholar]
  18. Johnson, K. E., Thorpe, C. M. & Sears, C. L. ( 2006; ). The emerging clinical importance of non-O157 Shiga toxin-producing Escherichia coli. Clin Infect Dis 43, 1587–1596.[CrossRef]
    [Google Scholar]
  19. Karch, H., Tarr, P. I. & Bielaszewska, M. ( 2005; ). Enterohaemorrhagic Escherichia coli in human medicine. Int J Med Microbiol 295, 405–418.[CrossRef]
    [Google Scholar]
  20. Karmali, M. A., Mascarenhas, M., Shen, S., Ziebell, K., Johnson, S., Reid-Smith, R., Isaac-Renton, J., Clark, C., Rahn, K. & Kaper, J. B. ( 2003; ). Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/or serious disease. J Clin Microbiol 41, 4930–4940.[CrossRef]
    [Google Scholar]
  21. Liu, Y. & Fratamico, P. ( 2006; ). Escherichia coli O antigen typing using DNA microarrays. Mol Cell Probes 20, 239–244.[CrossRef]
    [Google Scholar]
  22. Nelson, K. & Selander, R. K. ( 1994; ). Intergeneric transfer and recombination of the 6-phosphogluconate dehydrogenase gene (gnd) in enteric bacteria. Proc Natl Acad Sci U S A 91, 10227–10231.[CrossRef]
    [Google Scholar]
  23. Nielsen, E. M. & Andersen, M. T. ( 2003; ). Detection and characterization of verocytotoxin-producing Escherichia coli by automated 5′ nuclease PCR assay. J Clin Microbiol 41, 2884–2893.[CrossRef]
    [Google Scholar]
  24. Noller, A. C., McEllistrem, M. C., Stine, O. C., Morris, J. G., Jr, Boxrud, D. J., Dixon, B. & Harrison, L. H. ( 2003; ). Multilocus sequence typing reveals a lack of diversity among Escherichia coli O157 : H7 isolates that are distinct by pulsed-field gel electrophoresis. J Clin Microbiol 41, 675–679.[CrossRef]
    [Google Scholar]
  25. Paton, A. W. & Paton, J. C. ( 1999a; ). Direct detection of Shiga toxigenic Escherichia coli strains belonging to serogroups O111, O157, and O113 by multiplex PCR. J Clin Microbiol 37, 3362–3365.
    [Google Scholar]
  26. Paton, A. W. & Paton, J. C. ( 1999b; ). Molecular characterization of the locus encoding biosynthesis of the lipopolysaccharide O antigen of Escherichia coli serotype O113. Infect Immun 67, 5930–5937.
    [Google Scholar]
  27. Paton, A. W., Ratcliff, R. M., Doyle, R. M., Seymour-Murray, J., Davos, D., Lanser, J. A. & Paton, J. C. ( 1996; ). Molecular microbiological investigation of an outbreak of hemolytic-uremic syndrome caused by dry fermented sausage contaminated with Shiga-like toxin-producing Escherichia coli. J Clin Microbiol 34, 1622–1627.
    [Google Scholar]
  28. Perelle, S., Dilasser, F., Grout, J. & Fach, P. ( 2005; ). Detection of Escherichia coli serogroup O103 by real-time polymerase chain reaction. J Appl Microbiol 98, 1162–1168.[CrossRef]
    [Google Scholar]
  29. Prager, R., Annemuller, S. & Tschape, H. ( 2005; ). Diversity of virulence patterns among Shiga toxin-producing Escherichia coli from human clinical cases – need for more detailed diagnostics. Int J Med Microbiol 295, 29–38.[CrossRef]
    [Google Scholar]
  30. Reischl, U., Youssef, M. T., Kilwinski, J., Lehn, N., Zhang, W. L., Karch, H. & Strockbine, N. A. ( 2002; ). Real-time fluorescence PCR assays for detection and characterization of Shiga toxin, intimin, and enterohemolysin genes from Shiga toxin-producing Escherichia coli. J Clin Microbiol 40, 2555–2565.[CrossRef]
    [Google Scholar]
  31. Rozas, J., Sanchez-DelBarrio, J. C., Messeguer, X. & Rozas, R. ( 2003; ). DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19, 2496–2497.[CrossRef]
    [Google Scholar]
  32. Tarr, P. I., Schoening, L. M., Yea, Y. L., Ward, T. R., Jelacic, S. & Whittam, T. S. ( 2000; ). Acquisition of the rfb-gnd cluster in evolution of Escherichia coli O55 and O157. J Bacteriol 182, 6183–6191.[CrossRef]
    [Google Scholar]
  33. Tarr, C. L., Large, T. M., Moeller, C. L., Lacher, D. W., Tarr, P. I., Acheson, D. W. & Whittam, T. S. ( 2002; ). Molecular characterization of a serotype O121 : H19 clone, a distinct Shiga toxin-producing clone of pathogenic Escherichia coli. Infect Immun 70, 6853–6859.[CrossRef]
    [Google Scholar]
  34. Thompson, L. H., Giercke, S., Beaudoin, C., Woodward, D. L. & Wylie, J. L. ( 2005; ). Enhanced surveillance of non-O157 verotoxin-producing Escherichia coli in human stool samples from Manitoba. Can J Infect Dis Med Microbiol 16, 329–334.
    [Google Scholar]
  35. Wang, L., Curd, H., Qu, W. & Reeves, P. R. ( 1998; ). Sequencing of Escherichia coli O111 O-antigen gene cluster and identification of O111-specific genes. J Clin Microbiol 36, 3182–3187.
    [Google Scholar]
  36. Wang, G., Clark, C. G. & Rodgers, F. G. ( 2002; ). Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157 : H7 serotype, and components of the type 2 Shiga toxin family by multiplex PCR. J Clin Microbiol 40, 3613–3619.[CrossRef]
    [Google Scholar]
  37. Woodward, D. L., Clark, C. G., Caldeira, R. A., Ahmed, R. & Rodgers, F. G. ( 2002; ). Verotoxigenic Escherichia coli (VTEC): a major public health threat in Canada. Can J Infect Dis 13, 321–330.
    [Google Scholar]
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