@article{mbs:/content/journal/jmm/10.1099/jmm.0.47020-0, author = "Han, Thomas K. and Dao, My Lien", title = "Enhancement of salivary IgA response to a DNA vaccine against Streptococcus mutans wall-associated protein A in mice by plasmid-based adjuvants", journal= "Journal of Medical Microbiology", year = "2007", volume = "56", number = "5", pages = "675-680", doi = "https://doi.org/10.1099/jmm.0.47020-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.47020-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "HRP, horseradish peroxidase", keywords = "sIgA, salivary IgA", keywords = "IL, interleukin", keywords = "GST, glutathione S-transferase", abstract = "A specific salivary IgA (sIgA) response was obtained in mice by intranasal immunization with a naked DNA vaccine consisting of the Streptococcus mutans wall-associated protein A gene (wapA) inserted into the mammalian expression vector pcDNA3.1/V5/His-TOPO. In the present study, the vaccine, referred to as pcDNA-wapA, was administered with or without the cationic lipid DMRIE-C. No mucosal response was observed in mice immunized with the vaccine alone, whereas a weak and temporal sIgA response was obtained when the vaccine was mixed with DMRIE-C. To investigate the use of pcDNA containing the interleukin 5 (IL-5) gene (pcDNA-il-5) or the cholera toxin B gene (pcDNA-ctb) as genetic adjuvants, these constructs were used in co-immunization studies. The enhancement effect was transient with pcDNA-il-5, but longer lasting with pcDNA-ctb, thus supporting the use of the latter as a genetic adjuvant to DNA vaccine.", }