1887

Abstract

A specific salivary IgA (sIgA) response was obtained in mice by intranasal immunization with a naked DNA vaccine consisting of the wall-associated protein A gene () inserted into the mammalian expression vector pcDNA3.1/V5/His-TOPO. In the present study, the vaccine, referred to as pcDNA-, was administered with or without the cationic lipid DMRIE-C. No mucosal response was observed in mice immunized with the vaccine alone, whereas a weak and temporal sIgA response was obtained when the vaccine was mixed with DMRIE-C. To investigate the use of pcDNA containing the interleukin 5 (IL-5) gene (pcDNA-) or the cholera toxin B gene (pcDNA-) as genetic adjuvants, these constructs were used in co-immunization studies. The enhancement effect was transient with pcDNA-, but longer lasting with pcDNA-, thus supporting the use of the latter as a genetic adjuvant to DNA vaccine.

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2007-05-01
2019-11-14
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