1887

Abstract

A specific salivary IgA (sIgA) response was obtained in mice by intranasal immunization with a naked DNA vaccine consisting of the wall-associated protein A gene () inserted into the mammalian expression vector pcDNA3.1/V5/His-TOPO. In the present study, the vaccine, referred to as pcDNA-, was administered with or without the cationic lipid DMRIE-C. No mucosal response was observed in mice immunized with the vaccine alone, whereas a weak and temporal sIgA response was obtained when the vaccine was mixed with DMRIE-C. To investigate the use of pcDNA containing the interleukin 5 (IL-5) gene (pcDNA-) or the cholera toxin B gene (pcDNA-) as genetic adjuvants, these constructs were used in co-immunization studies. The enhancement effect was transient with pcDNA-, but longer lasting with pcDNA-, thus supporting the use of the latter as a genetic adjuvant to DNA vaccine.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47020-0
2007-05-01
2021-02-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/5/675.html?itemId=/content/journal/jmm/10.1099/jmm.0.47020-0&mimeType=html&fmt=ahah

References

  1. Curzon M. E. J., Preston A. J. 2004; Risk groups: nursing bottle caries/caries in elderly. Caries Res 38:24–33 [CrossRef]
    [Google Scholar]
  2. Dao M. L. 1985; An improved method of antigen detection on nitrocellulose: in situ staining of alkaline phosphatase conjugated antibody. J Immunol Methods 82:225–231 [CrossRef]
    [Google Scholar]
  3. De Magistris M. T., Pizza M., Douce G., Ghiara P., Dougan G., Rappuoli R. 1998; Adjuvant effect of non-toxic mutants of E. coli heat-labile enterotoxin following intranasal, oral and intravaginal immunization. Dev Biol Stand 92:123–126
    [Google Scholar]
  4. Donnelly J. J., Ulmer J. B., Liu M. A. 1997; DNA vaccines. Life Sci 60:163–172
    [Google Scholar]
  5. George-Chandy A., Eriksson K., Lebens M., Nordstrom I., Schon E., Holmgren J. 2001; Cholera toxin B subunit as a carrier protein promotes antigen presentation and increases CD40 and C86 expression on antigen-presenting cells. Infect Immun 69:5716–5725 [CrossRef]
    [Google Scholar]
  6. Han T. K., Dao M. L. 2005; Differential immunogenicity of a DNA vaccine containing the Streptococcus mutans wall-associated protein A gene versus that containing a truncated derivative antigen A lacking in the hydrophobic carboxyterminal region. DNA Cell Biol 24:574–581 [CrossRef]
    [Google Scholar]
  7. Han T. K., Yoder S., Cao C., Ugen K. E., Dao M. L. 2001; Expression of Streptococcus mutans wall-associated protein A gene in Chinese hamster ovary cells: prospect for a dental caries DNA vaccine. DNA Cell Biol 20:595–601 [CrossRef]
    [Google Scholar]
  8. Jia R., Guo J. H., Fan M. W., Bian Z., Chen Z., Fan B., Yu F., Xu Q. A. 2006; Immunogenicity of CTLA4 fusion anti-caries DNA vaccine in rabbits and monkeys. Vaccine 24:5192–5200 [CrossRef]
    [Google Scholar]
  9. Klavinskis L. S., Gao L., Barnfield C., Lehner T., Parker S. 1997; Mucosal immunization with DNA-liposome complexes. Vaccine 15:818–820 [CrossRef]
    [Google Scholar]
  10. Klavinskis L. S., Barnfield C., Gao L., Parker S. 1999; Intranasal immunization with plasmid DNA-lipid complexes elicits mucosal immunity in the female genital and rectal tracts. J Immunol 162:254–262
    [Google Scholar]
  11. Letvin N. L., Montefiori D. C., Yasutomi Y., Perry H. C., Davies M. E., Lekutis C., Alroy M., Freed D. L., Lord C. I. other authors 1997; Potent, protective anti-HIV immune responses generated by bimodal HIV envelope DNA plus protein vaccination. Proc Natl Acad Sci U S A 94:9378–9383 [CrossRef]
    [Google Scholar]
  12. Lowe D. B., Shearer M. H., Kennedy R. C. 2006; DNA vaccines: successes and limitations in cancer and infectious disease. J Cell Biochem 98:235–242 [CrossRef]
    [Google Scholar]
  13. Ochiya T., Takahama Y., Baba-Toriyama H., Tsukamoto M., Yasuda Y., Kikuchi H., Terada M. 1999; Evaluation of cationic liposome suitable for transfer into pregnant animals. Biochem Biophys Res Commun 258:328–365
    [Google Scholar]
  14. Okada E., Sasaki S., Ishii N., Aoki I., Yasuda T., Nichioka K., Fukushima J., Miyazaki J.-I., Wahren B., Okuda K. 1997; Intranasal immunization of a DNA vaccine with IL-12 and granulocyte-macrophage colony-stimulating factor (GM-CSF)-expressing plasmids in liposomes induces strong mucosal and cell-mediated immune responses against HIV-1 antigens. J Immunol 159:3638–3647
    [Google Scholar]
  15. Perrie Y., Frederik P. M., Gregoriadis G. 2001; Liposome-mediated DNA vaccination: the effect of vesicle composition. Vaccine 19:3301–3310 [CrossRef]
    [Google Scholar]
  16. Qian H., Dao M. L. 1993; Inactivation of the Streptococcus mutans wall-associated protein A gene ( wapA ) results in a decrease in sucrose dependent adherence and aggregation. Infect Immun 61:5021–5028
    [Google Scholar]
  17. Ramsay A. J., Kohonen-Corish M. 1993; Interleukin-5 expressed by a recombinant virus vector enhances specific mucosal IgA responses in vivo . Eur J Immunol 23:3141–3145 [CrossRef]
    [Google Scholar]
  18. Robinson H. L., Montefiori D. C., Johnson R. P., Manson K. H., Kalish M. L., Lifson J. D., Rizvi T. A., Lu S., Hu S. L. other authors 1999; Neutralizing antibody-independent containment of immunodeficiency virus challenges by DNA priming and recombinant pox virus booster immunizations. Nat Med 5:526–534 [CrossRef]
    [Google Scholar]
  19. Rugg-gunn A. 2001; Preventing the preventable – the enigma of dental caries. Br Dent J 191:478–488
    [Google Scholar]
  20. Shiver J. W., Davies M.-E., Perry H. C., Freed D. C., Liu M. A. 1996; Humoral and cellular immunities elicited by HIV-1 DNA vaccination. J Pharm Sci 85:1317–1324 [CrossRef]
    [Google Scholar]
  21. Whiley R. A., Russell R. R. B., Hardie J. M., Beighton D. 1988; Streptococcus downei sp. nov. for strains previously described as Streptococcus mutans serotype h. Int J Syst Bacteriol 38:25–29 [CrossRef]
    [Google Scholar]
  22. Whittle B. L., Smith R. M., Matthaei K. I., Young I. G., Verma N. K. 1997; Enhancement of the specific mucosal IgA response in vivo by interleukin-5 expressed by an attenuated strain of Salmonella serotype Dublin. J Med Microbiol 46:1029–1038 [CrossRef]
    [Google Scholar]
  23. Wu H. Y., Russell M. W. 1993; Induction of mucosal immunity by intranasal application of a surface protein with the cholera toxin B subunit. Infect Immun 61:314–322
    [Google Scholar]
  24. Yasuda Y., Matano K., Asai T., Tochikubo K. 1998; Affinity purification of recombinant cholera toxin B subunit oligomer expressed in Bacillus brevis for potential human use as a mucosal adjuvant. FEMS Immunol Med Microbiol 20:311–318 [CrossRef]
    [Google Scholar]
  25. Yoder S. C., Cao C., Ugen K. E., Dao M. L. 2000; High-level expression of a truncated wall-associated protein A from the dental cariogenic Streptococcus mutans . DNA Cell Biol 19:401–408 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47020-0
Loading
/content/journal/jmm/10.1099/jmm.0.47020-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error