1887

Abstract

In this study, the mechanisms leading to increased chromosomal AmpC -lactamase expression and the contributory roles of the outer-membrane protein OprF and penicillin-binding proteins were analysed in 33 characterized clinical isolates of . The genes and were analysed by PCR and DNA sequencing. Expression of the gene was assessed using real-time RT-PCR, and penicillin-binding proteins were analysed using a chemiluminescence assay. Several of the isolates with increased expression had major deletions affecting , although in some isolates the mechanism of increased expression could not be ascertained. Occasional isolates had increased expression of both and but remained susceptible to cephalosporins, suggesting that increased -lactamase activity could not offset increased outer-membrane permeability. There were no discernible changes in penicillin-binding proteins. Genomic deletions in were observed in selected clinical isolates of with increased expression of the AmpC -lactamase. For some isolates, cephalosporin resistance was dependent upon the interplay of and expression.

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2007-06-01
2020-10-01
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