1887

Abstract

is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, and . This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 10 c.f.u. could be detected directly in pus samples from infected sheep and goats (=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA.

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2007-04-01
2019-10-16
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