1887

Abstract

Cells of strain A5 adhered to plasticized polyvinyl chloride catheter tubing and grew at low nutrient concentration, consistent with reports of catheter-associated infection. Starting with initial cell densities of 1–2×10 c.f.u. ml, biofilms of approximately 350 c.f.u. cm formed within 24 h at room temperature. Growth rates of cells in biofilms were exponential and equal to 2.45 days doubling time. Rates were exponential for 1–2 weeks incubation and reached cell densities of 6.5×10 c.f.u. cm by 4 weeks. Cells grown in catheter biofilms were significantly more resistant to clarithromycin and rifamycin than cells grown in suspension.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46935-0
2007-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/2/250.html?itemId=/content/journal/jmm/10.1099/jmm.0.46935-0&mimeType=html&fmt=ahah

References

  1. Anwar H., van Biesen T., Dasgupta M., Lam K., Costerton J. W. 1989; Interaction of biofilm bacteria with antibiotics in a novel in vitro chemostat system. Antimicrob Agents Chemother 33:1824–1826 [CrossRef]
    [Google Scholar]
  2. Bardouniotis E., Huddleston W., Ceri H., Olson M. E. 2001; Characterization of biofilm growth and biocide susceptibility testing of Mycobacterium phlei using the MBEC™ assay system. FEMS Microbiol Lett 203:263–267
    [Google Scholar]
  3. Bardouniotis E., Ceri H., Olson M. E. 2003; Biofilm formation and biocide susceptibility testing of Mycobacterium fortuitum and Mycobacterium marinum . Curr Microbiol 46:28–32 [CrossRef]
    [Google Scholar]
  4. Beggs M. L., Crawford J. T., Eisenach K. D. 1995; Isolation and sequencing of the replication region of Mycobacterium avium plasmid pLR7. J Bacteriol 177:4836–4840
    [Google Scholar]
  5. Carter G., Wu M., Drummond D. C., Bermudez L. E. 2003; Characteristics of biofilm formation by clinical isolates of Mycobacterium avium . J Med Microbiol 52:747–752 [CrossRef]
    [Google Scholar]
  6. Davison M. B., McCormack J. G., Blacklock Z. M., Dawson D. J., Tilse M. H., Crimmins F. B. 1988; Bacteraemia caused by Mycobacterium neoaurum . J Clin Microbiol 26:762–764
    [Google Scholar]
  7. Donlon R. M. 2001; Biofilms and device-associated infections. Emerg Infect Dis 7:277–281 [CrossRef]
    [Google Scholar]
  8. Falkinham J. O. III, Norton C. D., LeChevallier M. W. 2001; Factors influencing numbers of Mycobacterium avium, Mycobacterium intracellulare , and other mycobacteria in drinking water distribution systems. Appl Environ Microbiol 67:1225–1231 [CrossRef]
    [Google Scholar]
  9. Flynn P. M., van Hooser B., Gigliotti F. 1988; Atypical mycobacteria infections of Hickman catheter exit sites. Pediatr Infect Dis 7:510–513 [CrossRef]
    [Google Scholar]
  10. George S. L., Schlessinger L. S. 1999; Mycobacterium neoaurum – an unusual cause of infection of vascular catheters: case report and review. Clin Infect Dis 28:682–683 [CrossRef]
    [Google Scholar]
  11. Hall-Stoodley L., Lappin-Scott H. 1998; Biofilm formation by the rapidly growing mycobacterial species Mycobacterium fortuitum . FEMS Microbiol Lett 168:77–84 [CrossRef]
    [Google Scholar]
  12. Hall-Stoodley L., Keevil C. W., Lappin-Scott H. M. 1999; Mycobacterium fortuitum and Mycobacterium chelonae biofilm formation under high and low nutrient conditions. J Appl Microbiol Symp 85:Suppl.60S–69S
    [Google Scholar]
  13. Holland D. J., Chen S. C. A., Chew W. W. K., Gilbert G. L. 1994; Mycobacterium neoaurum infection in a Hickman catheter in an immunosuppressed patient. Clin Infect Dis 18:1002–1003 [CrossRef]
    [Google Scholar]
  14. Kiska D. L., Turenne C. Y., Dubansky A. S., Domachowske J. B. 2004; First case report of catheter-related bacteremia due to “ Mycobacterium laticola ”. J Clin Microbiol 42:2855–2857 [CrossRef]
    [Google Scholar]
  15. Nichols W. W., Evans M. J., Slack M. P. E., Walmsley H. L. 1989; The penetration of antibiotics into aggregates of mucoid and nonmucoid Pseudomonas aeruginosa . J Gen Microbiol 135:1291–1303
    [Google Scholar]
  16. Nickel J. C., Ruseska I., Wright J. B., Costerton J. W. 1985; Tobramycin resistance of Pseudomonas aeruginosa cells growing as a biofilm on urinary catheter material. Antimicrob Agents Chemother 27:619–624 [CrossRef]
    [Google Scholar]
  17. Raad I. I., Vartivarian S., Khan A., Bodey G. P. 1991; Catheter-related infections caused by the Mycobacterium fortuitum complex: 15 cases and review. Rev Infect Dis 13:1120–1125 [CrossRef]
    [Google Scholar]
  18. Schelonka R. L., Asher D. P., McMahon D. P., Drehner D. M., Kuskie M. R. 1994; Catheter-related sepsis caused by Mycobacterium avium complex. Ped Infect Dis J 13:236–238 [CrossRef]
    [Google Scholar]
  19. Schulze-Röbbecke R., Fischeder R. 1989; Mycobacteria in biofilms. Zentralbl Hyg Umweltmed 188:385–390
    [Google Scholar]
  20. Schulze-Röbbecke R., Janning B., Fischeder R. 1992; Occurrence of mycobacteria in biofilm samples. Tubercle Lung Dis 73:141–144 [CrossRef]
    [Google Scholar]
  21. Steed K. A., Falkinham J. O. III 2006; Effect of growth in biofilms on chlorine susceptibility of Mycobacterium avium and Mycobacterium intracellulare . Appl Environ Microbiol 72:4007–4011 [CrossRef]
    [Google Scholar]
  22. Strahl E. D., Gillaspy G. E., Falkinham J. O. III 2001; Fluorescent acid-fast microscopy for measuring phagocytosis of Mycobacterium avium, Mycobacterium intracellulare , and Mycobacterium scrofulaceum by Tetrahymena pyriformis and their intracellular growth. Appl Environ Microbiol 67:4432–4439 [CrossRef]
    [Google Scholar]
  23. Torvinen E., Suomalainen S., Lehtola M. J., Miettinen I. T., Zacheus O., Paulin L., Katila M.-L., Martikainen P. J. 2004; Mycobacteria in water and loose deposits of drinking water distribution systems in Finland. Appl Environ Microbiol 70:1973–1981 [CrossRef]
    [Google Scholar]
  24. Van Oss C. J., Gillman C. F., Neumann A. W. 1975 Phagocytic Engulfment and Cell Adhesiveness New York: Marcel Dekker;
    [Google Scholar]
  25. Woo P. C. Y., Tsoi H.-W., Leung K.-W., Lum P. N. L., Leung A. S. P., Ma C.-H., Kam K.-M., Yuen K.-Y. 2000; Identification of Mycobacterium neoaurum isolated from a neutropenic patient with catheter-related bacteremia by 16S rRNA sequencing. J Clin Microbiol 38:3515–3517
    [Google Scholar]
  26. Woodley C. L., David H. L. 1976; Effect of temperature on the rate of the transparent to opaque colony type transition in Mycobacterium avium . Antimicrob Agents Chemother 9:113–119 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46935-0
Loading
/content/journal/jmm/10.1099/jmm.0.46935-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error